FastQCFastQC Report
Mon 6 Jun 2016
ERR522997_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522997_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1720851
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1284757.465782917870286No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT804124.672804327626273No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT515852.997644769942313No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC398852.3177486022903784No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT331231.924803483857696No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC329881.9169585280770969No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC325071.8890072411847394No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA311631.8109063480801066No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT295661.7181034267347957No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG277361.611760692820006No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC262241.5238971880772942No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA231261.3438699806084315No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC223371.298020572379596No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC217591.2644325394819191No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA181491.0546526108303391No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG162600.9448813406855097No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC161750.9399419240829101No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT139690.8117495355495623No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG134920.7840306917914451No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC123640.7184817279357713No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT110060.6395672838612989No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG100950.5866283600381439No Hit
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT66920.38887736358348285No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG62840.3651681638910051No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56640.3291394780838085No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG54760.3182146507745296No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA54680.31774976450604964No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51040.29659743929021165No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA50300.29229724130677204No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT47760.2775371022825335No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCCTGTC45280.26312562795965483No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCCTGTC43570.2531886839708958No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT42120.2447626203546966No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA40690.23645277830561742No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA39210.22785238233873822No Hit
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA36530.2122786923446597No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC35720.20757171887630016No Hit
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT31290.18182864175922261No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCCTGTCTCTTATACACATCT30730.17857443787986294No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCCTGTCTCTTATACACATCT27830.16172231064746453No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27640.16061820575982466No Hit
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC27000.156899115611985No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG24930.14487018341506613No Hit
GCTTATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT24570.14277819520690635No Hit
CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC23670.13754822468650685No Hit
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT23350.13568867961258704No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22510.1308073737935475No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA22200.12900593950318767No Hit
GCTTGCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT21380.12424085525126811No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCCTGTCTCTTATACA20110.11686078573914882No Hit
GCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTT20110.11686078573914882No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19200.11157270443518934No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG18650.10837661133938964No Hit
AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG18630.10826038977226965No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGCTGTCTCTTATACA18550.10779550350378969No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGCTGTCTCTTATACACAT18550.10779550350378969No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCCTGTCTCTT17800.10343719473679013No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCCTGTCTCTTATACA17770.10326286238611013No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA17750.10314664081899014No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACCTGTC17730.10303041925187013No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCCTGTCTCTTATACACAT17710.10291419768475017No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACTGTCTCTT17670.10268175455051018No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCCTGTC17470.1015195388793103No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTCTGTCTCTTATACA17230.10012488007387042No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCA5600.043.660531
CATTAAA5950.037.11949594
ACCCATG7250.034.3725363
GTATCAT19200.031.81729794
ATAATAG600.00415331131.3460253
GCTTATG12150.030.5720461
TTATGTA11600.030.400243
TACCCAT8250.029.6362422
TATGTAC11750.029.2118284
CTTATGT12100.029.1440322
GCTTATA4100.028.6701451
ATCATTA10550.027.17055994
TAATAGT1057.4032476E-526.8680214
TCTCGGG15950.025.92645594
CGTATCA25750.025.9138794
TCATTAA8050.024.51740894
CGTATCT1551.5498917E-624.2537894
TTATACT5200.023.5095183
TGTAGGA1401.9259964E-523.5095182
GCATCAT2653.6379788E-1123.05253294
Produced by FastQC (version 0.11.5)