FastQCFastQC Report
Mon 6 Jun 2016
ERR522997_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522997_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1720851
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1261747.332070004898739No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT1095236.364467347841271No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT801894.659845622892394No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT606023.521629705302783RNA PCR Primer, Index 33 (95% over 22bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT518003.0101385884077123No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC401872.335298058925497No Hit
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT344552.0022070475596085RNA PCR Primer, Index 33 (95% over 24bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC334321.9427597159777341No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC327381.9024308321870982No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT319801.8583828582486221No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT308851.7947515502504285No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA308031.789986465998509No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG266901.5509768132162518No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC265861.5449332917260123No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA228581.328296290614353No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC215931.25478614941096No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC215911.25466992784384No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA182341.0595920274329387No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG160390.9320388575187509No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC158080.9186152665163921No Hit
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT150290.8733469661231565RNA PCR Primer, Index 33 (96% over 27bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT143660.8348195166228802No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG136810.7950136298842839No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC122150.7098232211853321No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT105540.6133012096921814No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG104230.6056886970458221No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTG94960.5518200006857072RNA PCR Primer, Index 15 (95% over 24bp)
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG63780.37063057754564455No Hit
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT62390.36255317863080533No Hit
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC60070.34907147684488665No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA57000.3312314662919683No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG54800.3184470939087696No Hit
CTTTACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC54100.31437933905956994No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA48920.28427795317549287No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48310.28073319537833313No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT47880.2782344316852534No Hit
TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCT47550.2763167758277736RNA PCR Primer, Index 33 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47040.27335312586621385No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCCTGTC44640.25940653781181516No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCCTGTC43750.2542346780749757No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT41970.24389095860129667No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA40550.2356392273357775No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA40480.23523245185085753No Hit
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA40420.23488378714949754No Hit
ACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGC40060.23279179894133772RNA PCR Primer, Index 15 (96% over 25bp)
CTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC38350.22285485495257867No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC35200.20454995813118043No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG32270.18752349854810207RNA PCR Primer, Index 33 (96% over 29bp)
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT31230.18147997705786265No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCCTGTCTCTTATACACATCT30360.17642433888814313No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCCTGTCTCTTATACACATCT27550.1600952087077847No Hit
GCTTATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT27390.1591654361708248No Hit
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC26590.15451657348602524No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26080.15155292352446553No Hit
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT24930.14487018341506613No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG23700.13772255703718683No Hit
CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC22440.13040059830862755No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA21870.12708828364570787No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20690.12023121118562849No Hit
GCTTGCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT19760.11482690831454902No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGCTGTCTCTTATACA19700.11447824361318906No Hit
GCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTT19450.1130254740241892No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCCTGTCTCTTATACA19030.11058482111466943No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG18790.10919016230922955No Hit
AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG18790.10919016230922955No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18640.10831850055582964No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCCTGTCTCTTATACACAT18550.10779550350378969No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC18410.10698195253394976No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA18220.10587784764630988No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACTGTCTCTT17790.10337908395323013No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACCTGTC17770.10326286238611013No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTCTGTCTCTTATACA17670.10268175455051018No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGCTGTCTCTTATACACAT17530.10186820358067025No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCCTGTCTCTT17500.10169387122999028No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCCTGTC17500.10169387122999028No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTACA6750.080.1388551
CTATACA7300.063.1469351
GTACCCA4300.059.0710071
CTTAATA1700.058.1057931
CTTAACA1700.058.1057931
CTTATTA1800.054.8776931
GTATAAC352.9262135E-453.757741
ATTACAC1950.048.2090764
GAGATGT2550.047.960341
GCGTTAT405.654923E-447.038021
CGGAGAT804.609319E-947.038021
GATGTGT2600.047.003853
ATGTGTA2650.046.1169854
TACCCAT5600.045.3264582
TTATTAC2100.044.7668722
TGTGTAT2800.043.6451645
CTTATCA1750.043.0061951
ACCCATG5800.042.9517943
TATTACA2200.042.7307743
TTTACAC12850.042.0669252
Produced by FastQC (version 0.11.5)