##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR522996_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1799491 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.30878898532974 29.0 27.0 30.0 17.0 30.0 2 30.33641902071197 34.0 30.0 34.0 14.0 35.0 3 29.164826609302295 34.0 28.0 34.0 11.0 34.0 4 29.604730448776905 34.0 27.0 35.0 13.0 35.0 5 29.635598622054793 35.0 27.0 35.0 11.0 36.0 6 30.023603896879727 34.0 29.0 35.0 12.0 36.0 7 30.19557197007376 35.0 29.0 36.0 12.0 36.0 8 30.523538044924926 35.0 30.0 36.0 11.0 38.0 9 30.736835027238257 35.0 30.0 37.0 12.0 37.0 10-11 30.709713191119043 36.0 28.5 36.5 11.0 37.0 12-13 30.92232497967481 35.5 30.5 36.5 12.0 38.0 14-15 30.88971242423552 36.0 28.0 37.0 12.0 38.0 16-17 30.893072818924907 36.0 28.5 37.0 11.5 38.0 18-19 30.93634144321922 36.0 27.5 37.5 11.0 37.5 20-21 31.23649409749757 36.0 30.5 37.0 11.0 38.0 22-23 31.213223350380744 36.5 30.0 38.0 11.0 38.0 24-25 31.05910421335811 36.5 28.0 37.0 11.0 38.0 26-27 31.172262323068026 36.0 29.5 37.5 11.0 38.0 28-29 31.1122008945863 36.0 27.5 37.5 11.0 38.0 30-31 31.134671971129613 36.0 27.0 38.0 11.0 38.5 32-33 31.012195115174237 36.0 27.0 37.5 11.0 38.0 34-35 30.656555381493988 36.5 25.5 37.5 11.0 37.5 36-37 30.323340600203057 36.0 24.0 37.0 11.0 37.5 38-39 30.273857996511236 36.0 24.5 37.5 11.0 38.0 40-41 30.130980371671768 36.0 23.0 37.5 10.5 39.0 42-43 29.419636997350917 36.0 23.0 37.0 9.0 38.0 44-45 29.10950874441717 35.5 22.0 37.0 10.0 38.0 46-47 28.400017004808582 35.5 20.5 37.0 10.0 38.0 48-49 27.946704929338352 34.5 19.0 37.0 7.0 38.0 50-51 27.72946655470908 35.0 20.5 37.0 6.5 38.5 52-53 27.585590314149947 34.5 11.0 37.0 7.0 37.5 54-55 27.574197925969067 34.0 12.0 37.5 7.0 38.5 56-57 27.39767967719761 34.5 11.5 37.0 7.0 38.0 58-59 27.361109335917767 34.5 12.0 37.0 7.0 38.5 60-61 27.002051413427466 34.5 12.0 37.0 7.0 38.0 62-63 26.825527329672667 34.5 12.0 36.5 7.0 37.5 64-65 26.635024570836975 33.5 11.5 36.5 7.0 38.0 66-67 26.75230940304786 33.5 11.0 37.0 7.0 38.0 68-69 26.519227937233364 33.0 11.0 37.5 7.0 38.0 70-71 26.016116223976667 32.0 10.5 36.5 7.0 38.0 72-73 25.985064387651843 32.5 11.0 37.0 7.0 38.0 74-75 25.567994227256484 32.5 11.0 36.5 6.5 38.0 76-77 25.215872432815722 31.5 11.0 37.0 6.5 37.5 78-79 25.075528302169893 31.5 11.0 36.0 7.0 37.5 80-81 24.86803546113873 31.0 10.5 37.0 6.5 37.0 82-83 24.821632617223425 30.0 11.0 37.0 7.0 38.0 84-85 24.399646344438512 29.5 11.0 36.5 6.5 37.0 86-87 24.30220573484391 28.5 11.0 37.0 6.5 38.0 88-89 24.083022643625334 26.5 10.5 36.5 6.5 37.5 90-91 23.855316586745918 26.0 10.5 36.0 6.0 37.5 92-93 23.754338865823726 25.0 10.5 37.0 7.0 37.0 94-95 23.52366585884564 25.0 10.5 36.0 6.0 37.5 96-97 23.470505826369788 22.5 10.5 36.0 7.0 37.0 98-99 23.549283936402016 24.0 11.5 36.0 7.0 37.5 100 23.08815214969122 21.0 11.0 35.0 7.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07123143852895453 1101 2 0.3271300913698667 1101 3 0.4072656170229969 1101 4 0.3500066740823655 1101 5 0.1966172697339772 1101 6 0.27939171607016533 1101 7 0.46139963611191703 1101 8 -0.20303002468286735 1101 9 0.2940652205854484 1101 10-11 0.28205566129639337 1101 12-13 0.10082464148717563 1101 14-15 0.2223838313526798 1101 16-17 0.20380140259531387 1101 18-19 0.04342250135379189 1101 20-21 0.18375002654862627 1101 22-23 0.27754554457813185 1101 24-25 0.27782712864039993 1101 26-27 0.35212364529286333 1101 28-29 0.25212495476941044 1101 30-31 0.1370356343269883 1101 32-33 0.26987802889030377 1101 34-35 -0.646062981406029 1101 36-37 -0.24602806019365175 1101 38-39 -0.3789863613652571 1101 40-41 -0.08278569128349744 1101 42-43 -0.02166601728903217 1101 44-45 -0.3443165544111011 1101 46-47 -0.11574375684299554 1101 48-49 -0.3197804786898679 1101 50-51 -0.4242322461274597 1101 52-53 -0.34875329604640015 1101 54-55 0.017355191462584685 1101 56-57 -0.24478478302023277 1101 58-59 -0.537290325144486 1101 60-61 -0.08451383761525832 1101 62-63 -0.24468914597025204 1101 64-65 -0.11411182416896182 1101 66-67 0.09116198720473889 1101 68-69 -0.7446995842639375 1101 70-71 -0.5121193896443401 1101 72-73 -0.3702473581411496 1101 74-75 -0.6578717728699921 1101 76-77 -1.0783282470134878 1101 78-79 -0.5213230118705212 1101 80-81 -0.7810621606160737 1101 82-83 -1.4702077436587793 1101 84-85 -1.407772358520397 1101 86-87 -1.2008952119206526 1101 88-89 -1.2930325710845345 1101 90-91 -1.0764913218079961 1101 92-93 -0.9678622256051561 1101 94-95 -1.1012025060886366 1101 96-97 -1.2165340560636224 1101 98-99 -1.5151536262160903 1101 100 -1.8921719449361234 1102 1 0.10746240863732126 1102 2 -0.09492256273529875 1102 3 0.2502561314546661 1102 4 -0.01480450707524028 1102 5 0.13522435299925206 1102 6 -0.030825584288702856 1102 7 -0.2326054957301089 1102 8 0.0438073725648529 1102 9 -1.5211943247716668 1102 10-11 0.002771906822577108 1102 12-13 0.043331435354051706 1102 14-15 -0.044541736261720644 1102 16-17 -0.12878724160179544 1102 18-19 -0.2231291709747154 1102 20-21 -0.05904930161151256 1102 22-23 0.033970340624286166 1102 24-25 0.15492464673382855 1102 26-27 0.007722839195302811 1102 28-29 0.1373139459137782 1102 30-31 0.05224600470042162 1102 32-33 -0.14249694018148773 1102 34-35 -0.39683799568986444 1102 36-37 -0.06305559611688949 1102 38-39 -0.17702621963654153 1102 40-41 -0.10214002753838614 1102 42-43 -0.1637591485741794 1102 44-45 -0.07333878894717216 1102 46-47 -0.14716767414907395 1102 48-49 -0.08398505351407337 1102 50-51 -0.0779555884273897 1102 52-53 -0.05172054281935701 1102 54-55 0.047834893945321255 1102 56-57 0.05970175364803865 1102 58-59 0.3278952682049301 1102 60-61 0.08435002383741264 1102 62-63 -0.05347168778277478 1102 64-65 0.10939743637298704 1102 66-67 0.05894065635806456 1102 68-69 0.23443671417962264 1102 70-71 0.1002270117599906 1102 72-73 -0.004069305415367097 1102 74-75 -0.32260641749027386 1102 76-77 -0.14374786090543523 1102 78-79 -0.006380467687129965 1102 80-81 -0.18808146739698373 1102 82-83 -0.30041190082298996 1102 84-85 -0.39880576373811394 1102 86-87 -0.6489480617608585 1102 88-89 -0.5552522271560072 1102 90-91 -0.7372692238729321 1102 92-93 -0.6324719512935211 1102 94-95 -0.2810787036735185 1102 96-97 -0.49764369456718427 1102 98-99 -1.1669096245647488 1102 100 -0.7213393611163816 1103 1 -0.1667711260916498 1103 2 0.11091030915645916 1103 3 -0.1937881314325267 1103 4 -0.19737833374481184 1103 5 -0.1860987541969834 1103 6 -0.2582969367893284 1103 7 0.09310238751276856 1103 8 -0.4344121297883312 1103 9 0.03186849796717084 1103 10-11 -0.3633370926195987 1103 12-13 -0.11730383143656198 1103 14-15 -0.08024758483741934 1103 16-17 -0.39993275922317295 1103 18-19 -0.5194866387836186 1103 20-21 -0.201001476008436 1103 22-23 -0.24472136838006975 1103 24-25 -0.2931469598953633 1103 26-27 -0.2322507920642849 1103 28-29 -0.7656109222937282 1103 30-31 -0.3674720368573112 1103 32-33 -0.222668971974187 1103 34-35 -0.1341658768434506 1103 36-37 -0.07196955495789226 1103 38-39 -0.1875204594118287 1103 40-41 -0.2725981963515771 1103 42-43 -0.08077255074935863 1103 44-45 -0.3779902566044946 1103 46-47 -0.17622193626473148 1103 48-49 -0.21926652377276667 1103 50-51 -0.20438028218608295 1103 52-53 -0.4394468568695551 1103 54-55 -0.3880263199043448 1103 56-57 -0.22604181820336677 1103 58-59 -0.268878589462588 1103 60-61 -0.29420868074871365 1103 62-63 -0.22895507486181899 1103 64-65 -0.3454612873990932 1103 66-67 -0.2540464603751573 1103 68-69 -0.3966035333123763 1103 70-71 -0.32075416210641805 1103 72-73 -0.6419789443438724 1103 74-75 -0.40617473895130374 1103 76-77 -1.0476362942321913 1103 78-79 -1.0678071039384776 1103 80-81 -0.7343100615716907 1103 82-83 -0.41423036344758657 1103 84-85 -0.7724333022073182 1103 86-87 -0.7230951231347511 1103 88-89 -0.8600281304150954 1103 90-91 -0.8527621902742979 1103 92-93 -0.8876982465674246 1103 94-95 -0.9377907497908176 1103 96-97 -1.395655831561733 1103 98-99 -0.8233702390752029 1103 100 -0.6179465194578704 1104 1 0.04180760546319462 1104 2 -0.07160348072171274 1104 3 0.3262458351455173 1104 4 -0.06390823077951069 1104 5 0.2779969152616424 1104 6 0.25298688672653924 1104 7 0.4530895744039327 1104 8 -0.027042092730294343 1104 9 -0.013671316729489291 1104 10-11 0.11627769849647152 1104 12-13 0.13139512496598016 1104 14-15 0.0639150179864636 1104 16-17 0.09490812275020133 1104 18-19 0.0316977186994869 1104 20-21 0.02551758118880798 1104 22-23 0.13328902155150857 1104 24-25 0.12677834520528464 1104 26-27 0.04121074690322146 1104 28-29 -0.023042262316014472 1104 30-31 0.28609188858460044 1104 32-33 -0.4014559099700534 1104 34-35 0.25669083865949105 1104 36-37 0.15615475850810512 1104 38-39 0.1263375623967704 1104 40-41 0.10916272471888178 1104 42-43 0.2728853681860173 1104 44-45 0.19653494141328665 1104 46-47 0.23761977316711125 1104 48-49 0.22585614135386933 1104 50-51 -0.05427747474710998 1104 52-53 0.21134725331002713 1104 54-55 0.18686557771181356 1104 56-57 0.2564671042720654 1104 58-59 0.163963421222018 1104 60-61 0.28500106352847254 1104 62-63 0.16066538612068726 1104 64-65 0.1783590580812593 1104 66-67 0.13561543930635622 1104 68-69 -0.06846001333826734 1104 70-71 0.015219956466147266 1104 72-73 -0.07306737452756096 1104 74-75 -0.07602769076796179 1104 76-77 -0.18514699922943834 1104 78-79 -0.12853847724327494 1104 80-81 -0.3422897737108599 1104 82-83 0.04254019376478624 1104 84-85 -0.4042560524477281 1104 86-87 -0.6650950863003509 1104 88-89 -0.476907625362859 1104 90-91 -0.4917304850652542 1104 92-93 -0.4066840771633906 1104 94-95 -0.578631021365581 1104 96-97 -0.6436450680241066 1104 98-99 -0.47539632835389156 1104 100 -0.6132225077708426 1105 1 0.1846082917485532 1105 2 0.09566930235980564 1105 3 -0.03141837742644782 1105 4 0.11698186828167323 1105 5 0.35049587771226953 1105 6 0.21320861854192685 1105 7 0.5614068515674226 1105 8 -0.2930144379627784 1105 9 -0.16858143521794844 1105 10-11 0.21297218448907174 1105 12-13 0.0954292512279018 1105 14-15 0.3182727253636486 1105 16-17 0.051911053467481594 1105 18-19 0.1986902515413611 1105 20-21 -0.015773686578224755 1105 22-23 0.1677094423137504 1105 24-25 0.15932446259639832 1105 26-27 0.1229809883884414 1105 28-29 0.09475987877663528 1105 30-31 0.02151168202114917 1105 32-33 -0.2066946230635125 1105 34-35 -0.12504212139054616 1105 36-37 0.019866652948568486 1105 38-39 -0.047565624814229324 1105 40-41 0.09735784283324733 1105 42-43 -0.012947174861885458 1105 44-45 -0.2326491962632815 1105 46-47 -0.223758290641058 1105 48-49 -0.06397504344758076 1105 50-51 0.024158540270335038 1105 52-53 -0.04132879276036405 1105 54-55 0.1668853183276653 1105 56-57 -0.0979592827987581 1105 58-59 -0.1402841044865042 1105 60-61 -0.12794486687993967 1105 62-63 -0.15461069793456161 1105 64-65 0.013435548970971212 1105 66-67 0.14103531587474905 1105 68-69 -0.02699960424006065 1105 70-71 -0.04473425067666881 1105 72-73 0.04376228384532155 1105 74-75 -0.22212423497715506 1105 76-77 -0.11785992386530353 1105 78-79 -0.23189422206943533 1105 80-81 -0.4103995346044904 1105 82-83 -0.29979722432636535 1105 84-85 -0.11343655790214058 1105 86-87 -0.2021818011280665 1105 88-89 -0.2961868275017814 1105 90-91 -0.8447073405200776 1105 92-93 -0.39279242609460496 1105 94-95 -0.866970948759505 1105 96-97 -0.6908837798386145 1105 98-99 -0.7577058397240926 1105 100 -0.5655109036523633 1106 1 0.03186218453096501 1106 2 0.0046306644878661984 1106 3 0.13445552066012212 1106 4 0.15839554057354022 1106 5 -0.03827415404923329 1106 6 0.026670393709078866 1106 7 0.06781974346284514 1106 8 0.1007659927399196 1106 9 0.32970327878261685 1106 10-11 0.1442869875309718 1106 12-13 -0.06114048131817995 1106 14-15 -0.07745964814550632 1106 16-17 0.04814453031762511 1106 18-19 -0.08253401282395956 1106 20-21 -0.05778264908239805 1106 22-23 -0.05082043671845682 1106 24-25 0.15396447142820335 1106 26-27 0.24772624922485065 1106 28-29 -0.02436890693919125 1106 30-31 -0.07788163905425094 1106 32-33 0.014239733540861721 1106 34-35 -0.15003923455818224 1106 36-37 -0.09896202909508034 1106 38-39 0.03874749054105564 1106 40-41 -0.1114307217575643 1106 42-43 -0.08569614353309518 1106 44-45 0.07054973123090846 1106 46-47 0.24012299519849023 1106 48-49 0.42323835120080133 1106 50-51 0.23556095708832459 1106 52-53 0.36522920211566756 1106 54-55 0.37169132319277054 1106 56-57 0.4071408934566314 1106 58-59 0.3439734593315258 1106 60-61 0.15536568902880177 1106 62-63 0.31218787476475995 1106 64-65 0.19450925392527907 1106 66-67 0.263601335510284 1106 68-69 0.05970117146131315 1106 70-71 0.17831685196639668 1106 72-73 0.27414181664798676 1106 74-75 0.11138103936074373 1106 76-77 -0.037866116279339224 1106 78-79 0.0649243300477238 1106 80-81 0.12980119224279463 1106 82-83 -0.17252630240534828 1106 84-85 0.0011483219485342033 1106 86-87 -0.20811551057441946 1106 88-89 -0.059836786070977865 1106 90-91 -0.218918061517563 1106 92-93 -0.17678646335821568 1106 94-95 -0.4301001037360841 1106 96-97 -0.036595755180620415 1106 98-99 -0.284046757495684 1106 100 -0.35176317346396146 1107 1 0.027162246596706296 1107 2 0.15755159424026743 1107 3 0.14420695553704022 1107 4 0.17997943822674856 1107 5 -0.004319911484216732 1107 6 0.05896157647066502 1107 7 0.04402986811049914 1107 8 0.0734709135588858 1107 9 0.11173511989431262 1107 10-11 0.10139474272756033 1107 12-13 -0.13684432322552453 1107 14-15 0.02151475634437361 1107 16-17 0.06709881694554198 1107 18-19 -0.08076063839882508 1107 20-21 -0.013164082738143179 1107 22-23 -0.16862685328409555 1107 24-25 0.18867344855200585 1107 26-27 -0.06660542986126217 1107 28-29 0.04156839281065672 1107 30-31 0.13429696928823986 1107 32-33 -0.08849701328251669 1107 34-35 -0.10246987010888375 1107 36-37 -0.21999872845642088 1107 38-39 0.07642064200791054 1107 40-41 0.13970122271310714 1107 42-43 0.08939267468729284 1107 44-45 -0.10926876738236757 1107 46-47 -0.21169579548887896 1107 48-49 -0.03388104919123336 1107 50-51 0.07037858073826797 1107 52-53 0.26856337893883264 1107 54-55 0.2500300322001081 1107 56-57 0.17123976305436273 1107 58-59 0.17182473822577293 1107 60-61 9.201828782501309E-4 1107 62-63 0.25610178089063496 1107 64-65 0.5582782497569312 1107 66-67 0.2869345303602131 1107 68-69 0.2971825863588542 1107 70-71 0.27797627720990903 1107 72-73 0.25348339729757186 1107 74-75 0.01486628898966913 1107 76-77 0.2771294963145934 1107 78-79 -0.04823722721076251 1107 80-81 0.28856061383537934 1107 82-83 0.11199446318127215 1107 84-85 0.10507303607472096 1107 86-87 0.15352933866121887 1107 88-89 -0.11599967528803035 1107 90-91 -0.1872901827937774 1107 92-93 0.056201637140450345 1107 94-95 -0.26167548465987167 1107 96-97 0.019338793785220076 1107 98-99 -0.0688564498233255 1107 100 -0.19025973466922252 1108 1 -0.05349598433050318 1108 2 0.11878230135263479 1108 3 -0.033723329994145956 1108 4 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1.471752376989226 2316 100 1.3920136446678448 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 94.0 1 51.0 2 42.0 3 50.0 4 50.0 5 51.0 6 86.0 7 183.0 8 246.0 9 304.0 10 336.0 11 395.0 12 700.0 13 3048.0 14 11228.0 15 27625.0 16 49292.0 17 72103.0 18 91903.0 19 100540.0 20 102142.0 21 102350.0 22 95242.0 23 71437.0 24 50329.0 25 40691.0 26 34596.0 27 33150.0 28 33587.0 29 35344.0 30 38702.0 31 46251.0 32 57233.0 33 75709.0 34 104523.0 35 164373.0 36 282667.0 37 65913.0 38 6925.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.86307095454596 16.11623072733134 14.680020898409277 17.340677419713423 2 16.805813475074782 22.86448198022885 35.314915099689756 25.01478944500662 3 27.106586900950646 26.35689225176481 30.473900014016102 16.062620833268443 4 13.477056110406153 16.579058189867382 37.049957693144286 32.89392800658218 5 10.871760181531636 37.03368893848607 33.04073836513604 19.053812514846243 6 25.81022399500862 31.752061253440054 16.72296987319684 25.71474487835449 7 20.93020281493089 35.22172404646732 22.201987500688137 21.646085637913654 8 26.23684637815259 22.95858902869497 21.559706488786475 29.244858104365967 9 25.968342017861346 18.269926362690576 23.555209076467033 32.20652254298105 10-11 25.084280694947104 23.414843352838776 24.350915657044766 27.14996029516935 12-13 31.225803151900056 23.267583506227528 25.424869412070795 20.081743929801622 14-15 26.753543139644943 23.634635136881872 30.652476966291353 18.959344757181835 16-17 18.65676371867058 27.772798565987006 30.84509579453672 22.725341920805697 18-19 18.627237569567637 28.16866873003045 32.55155142882799 20.652542271573925 20-21 22.627704351984164 23.543639386374124 30.680014901389498 23.148641360252213 22-23 20.20433688369433 24.44911858139017 31.78697611603128 23.559568418884215 24-25 23.06827968377926 25.005323534693602 29.91206577053369 22.014331010993445 26-27 21.580449433079597 22.063247778660575 35.09624553188437 21.260057256375458 28-29 19.17743634791746 27.764317027540176 32.780843357082695 20.277403267459672 30-31 20.772679624718165 24.623096161314148 31.716741215815965 22.88748299815172 32-33 19.250501989055195 25.548880280891506 34.07627342893547 21.12434430111783 34-35 20.52747352931857 24.389327341446915 32.80040825470557 22.282790874528942 36-37 19.79559159787137 23.71027403332914 35.22611077898151 21.268023589817993 38-39 19.864925709140028 24.52334895303528 34.1425561836788 21.46916915414589 40-41 18.543686945616184 26.19001425359842 33.134640913831326 22.131657886954066 42-43 18.436006742673264 26.27941057492049 31.06281067606615 24.221772006340093 44-45 19.57233421381899 23.737689420269707 32.231780898095366 24.458195467815933 46-47 20.67125575559514 26.044961354991813 32.16748524637757 21.116297643035473 48-49 19.9075871428492 25.12695296205038 31.849758886707342 23.115701008393078 50-51 16.538689030333384 25.78550312271365 33.87143913454434 23.80436871240863 52-53 18.57187384523244 26.7075545328337 29.340556646275562 25.3800149756583 54-55 17.86970061518418 23.783331280587877 30.487106766025107 27.859861338202833 56-57 20.80181446389475 22.1949364470622 29.713375194172542 27.28987389487051 58-59 22.506589346479693 22.00565170394677 29.453788134201147 26.033970815372392 60-61 19.64117225438217 22.88260586449578 31.82422909925376 25.651992781868294 62-63 18.44120010983257 22.977685534227632 31.507941183990678 27.073173171949115 64-65 18.656619765440137 25.155653410812167 29.66169528117668 26.526031542571022 66-67 20.509420153494258 23.01966362014855 27.829235622386665 28.641680603970528 68-69 20.473061205656766 22.745001353652388 29.01535825647349 27.76657918421736 70-71 22.91999694246979 22.666666666666664 28.467412981995317 25.945923408868225 72-73 22.308282185142968 20.75281019734127 31.50408893684307 25.434818680672695 74-75 21.781648929294313 23.845944923380834 29.065272029104804 25.307134118220056 76-77 22.58445068831439 21.47625951915283 29.203243155494228 26.73604663703855 78-79 23.49402127467397 21.45646056681512 29.981337761504097 25.068180397006817 80-81 25.148130681260795 20.423456018506293 29.148055090912678 25.280358209320237 82-83 24.030989440546556 19.738760728070663 30.605351786786688 25.624898044596094 84-85 24.364430448201517 21.077687855745815 29.356307452202714 25.20157424384995 86-87 25.492753897066102 20.748688009053044 28.044897269524792 25.713660824356065 88-89 26.212438271347622 20.225988700564972 26.82384719917964 26.737725828907767 90-91 28.249369048482592 18.261922767254276 26.802425810912705 26.68628237335043 92-93 29.104310425817083 18.9474676060707 27.35376800226364 24.59445396584858 94-95 28.444255687222363 18.380305530988085 27.44308886370067 25.732349918088875 96-97 27.716231671778395 18.268340033936134 28.405404766901015 25.610023527384456 98-99 28.97455759499107 19.272790643185132 26.23689822732262 25.515753534501183 100 29.571907432834326 16.567699329038142 26.734411393566106 27.12598184456143 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 13250.0 1 7418.0 2 1744.5 3 1622.0 4 1025.5 5 937.5 6 1693.0 7 3065.5 8 3929.0 9 3444.0 10 2132.5 11 1166.5 12 1013.0 13 1038.0 14 1056.5 15 1109.0 16 1201.0 17 1363.5 18 1694.0 19 2067.0 20 2601.0 21 3291.5 22 4195.0 23 5594.0 24 7414.5 25 9553.5 26 12195.5 27 15257.5 28 18731.0 29 22256.0 30 25165.0 31 27617.0 32 29341.5 33 30333.0 34 30458.0 35 29181.0 36 27052.5 37 24921.0 38 22522.0 39 20129.5 40 18106.0 41 16723.5 42 15863.5 43 15427.5 44 16197.5 45 19564.0 46 34756.0 47 73204.5 48 130816.5 49 158676.5 50 154669.5 51 133714.5 52 102926.0 53 81109.0 54 56685.0 55 37980.5 56 29252.5 57 25551.5 58 23848.5 59 22971.0 60 22287.5 61 21272.5 62 20195.0 63 19143.5 64 17902.0 65 16594.0 66 15259.0 67 13734.5 68 12287.0 69 10915.5 70 9389.5 71 7940.0 72 6717.0 73 5602.5 74 4614.0 75 3695.0 76 2878.0 77 2231.0 78 1703.0 79 1235.0 80 891.0 81 640.0 82 434.5 83 288.0 84 181.5 85 111.5 86 66.5 87 41.5 88 22.5 89 9.0 90 2.0 91 0.5 92 2.0 93 2.0 94 0.5 95 0.0 96 1.0 97 1.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017282664931361144 2 0.05062542685681673 3 0.08663560973630877 4 0.10614112546270028 5 0.10547427022419117 6 0.0662409536919051 7 0.06401810289687473 8 0.06262882114998075 9 0.06012811400557158 10-11 0.03287040613151163 12-13 0.0390666027226588 14-15 0.04270652089952103 16-17 0.050125285427934894 18-19 0.053987488684300165 20-21 0.05562684114563507 22-23 0.05232035058802739 24-25 0.04856928987141364 26-27 0.05079214066644401 28-29 0.04701329431489237 30-31 0.043873517566911976 32-33 0.03595461160961628 34-35 0.03412075970371622 36-37 0.027479992953562977 38-39 0.026729780810240227 40-41 0.03595461160961628 42-43 0.060822754879018566 44-45 0.06896394591581731 46-47 0.08102291147885708 48-49 0.08785817767357547 50-51 0.08088398330416767 52-53 0.06888058901100366 54-55 0.06037818472001249 56-57 0.046290867806507506 58-59 0.03136998184486613 60-61 0.0228120062839992 62-63 0.02161722398167037 64-65 0.025673926682600802 66-67 0.036760395022814786 68-69 0.03634361049874659 70-71 0.03631582486380871 72-73 0.03064755533648126 74-75 0.049319502014736394 76-77 0.04162288113694372 78-79 0.022423007394868882 80-81 0.0177550207253051 82-83 0.016532452788038395 84-85 0.015448813025461089 86-87 0.017616092550615702 88-89 0.015893383184467164 90-91 0.023478861522508308 92-93 0.03445418732297077 94-95 0.032787049226698 96-97 0.029091559779960002 98-99 0.030314127717226705 100 0.032286907797816164 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1799491.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.079654684481355 #Duplication Level Percentage of deduplicated Percentage of total 1 92.86745631872262 43.72167774911617 2 3.2250809352501095 3.036713935221586 3 1.1278324902910535 1.5929389255452442 4 0.6382049524051372 1.2018587510863887 5 0.39232343871468955 0.9235226009657933 6 0.2650917297189039 0.7488256256926715 7 0.2017908656636818 0.6650170991750092 8 0.1580396822530834 0.5952362933536258 9 0.11448782225665174 0.4851042423679298 >10 0.8180859320657093 7.41984060872485 >50 0.09025584805088212 2.9682037679573026 >100 0.08056028372571301 7.8174502763843785 >500 0.011339836844715753 3.7735551072776237 >1k 0.006260534924686822 5.793050799056853 >5k 0.0015356029060552582 5.495748822732247 >10k+ 0.0016537262065210473 13.761255395342351 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 48149 2.6757010732479354 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 31678 1.7603866871243035 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 20871 1.1598279735769725 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18257 1.0145646741217378 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16981 0.9436557337602689 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 14527 0.8072838374851555 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 13582 0.7547689874525629 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 13160 0.7313179115649926 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 12591 0.6996978590056855 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 12518 0.6956411563047551 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 12304 0.6837489045513426 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 11635 0.6465717250044596 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10695 0.5943347313212458 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 10503 0.5836650475051001 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9405 0.5226477931815163 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 9242 0.5135896761917675 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 9104 0.5059208409489128 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9032 0.501919709517858 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 8384 0.4659095266383661 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 8326 0.462686392985572 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 8125 0.4515165677405444 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 7529 0.4183960908945919 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 6873 0.38194133785609374 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 6444 0.358101263079393 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 6116 0.33987388656014395 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 5228 0.29052659891046967 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 5015 0.27868991842693297 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 4629 0.2572394082548899 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 4463 0.24801457745551378 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 4345 0.24145716761017422 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 4159 0.23112091141328298 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 3816 0.2120599658458975 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 3639 0.20222385107788815 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3634 0.20194599472850935 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 3224 0.179161774079448 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 3058 0.16993694328007197 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 2944 0.16360181851423541 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 2817 0.156544267240014 No Hit GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 2596 0.14426301659747118 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 2544 0.14137331056393168 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 2398 0.1332599051620708 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2234 0.1241462169024463 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 2183 0.12131208213878258 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 2080 0.11558824134157938 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 2058 0.11436567340431267 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1979 0.10997554308412767 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCCTGTC 1901 0.10564098403381846 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1848 0.10269570673040321 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.6671380962727793E-4 0.0 2 0.0 0.0 0.0 2.7785634937879655E-4 0.0 3 0.0 0.0 0.0 2.7785634937879655E-4 0.0 4 0.0 0.0 0.0 2.7785634937879655E-4 0.0 5 0.0 0.0 0.0 3.3342761925455585E-4 0.0 6 0.0 0.0 0.0 3.3342761925455585E-4 0.0 7 0.0 0.0 0.0 3.889988891303152E-4 0.0 8 0.0 0.0 0.0 5.001414288818338E-4 0.0 9 0.0 0.0 0.0 6.668552385091117E-4 0.0 10-11 0.0 5.5571269875759314E-5 0.0 0.0011392110324530658 2.7785634937879657E-5 12-13 0.0 5.5571269875759314E-5 0.0 0.0015282099215833812 5.5571269875759314E-5 14-15 0.0 5.5571269875759314E-5 0.0 0.0020561369854030943 1.1114253975151863E-4 16-17 0.0 5.5571269875759314E-5 0.0 0.003139776747980401 1.1114253975151863E-4 18-19 0.0 5.5571269875759314E-5 0.0 0.004640201034625903 1.1114253975151863E-4 20-21 0.0 5.5571269875759314E-5 0.0 0.02428464493570682 1.3892817468939828E-4 22-23 0.0 5.5571269875759314E-5 0.0 0.08885846053133914 1.9449944456515758E-4 24-25 0.0 5.5571269875759314E-5 0.0 0.19113738273767417 2.2228507950303725E-4 26-27 0.0 8.335690481363898E-5 0.0 0.3594627591913491 2.2228507950303725E-4 28-29 0.0 1.3892817468939828E-4 0.0 0.8542137748952343 2.2228507950303725E-4 30-31 0.0 1.6671380962727793E-4 0.0 1.6850598308077118 2.2228507950303725E-4 32-33 0.0 1.9449944456515758E-4 0.0 2.956252629215706 2.5007071444091693E-4 34-35 0.0 2.2228507950303725E-4 0.0 4.695966803946227 2.7785634937879655E-4 36-37 0.0 2.2228507950303725E-4 0.0 6.592753172980582 2.7785634937879655E-4 38-39 0.0 2.2228507950303725E-4 0.0 8.612046406456049 2.7785634937879655E-4 40-41 0.0 2.5007071444091693E-4 0.0 10.528810647010737 2.7785634937879655E-4 42-43 0.0 2.7785634937879655E-4 0.0 12.089752046550942 2.7785634937879655E-4 44-45 0.0 2.7785634937879655E-4 0.0 13.613627409084014 3.056419843166762E-4 46-47 0.0 2.7785634937879655E-4 0.0 15.299882022194053 3.3342761925455585E-4 48-49 0.0 2.7785634937879655E-4 0.0 16.606696004592408 3.3342761925455585E-4 50-51 0.0 2.7785634937879655E-4 0.0 17.735070639419703 3.3342761925455585E-4 52-53 0.0 2.7785634937879655E-4 0.0 18.656998006658547 3.3342761925455585E-4 54-55 0.0 2.7785634937879655E-4 0.0 19.653779874420046 3.889988891303152E-4 56-57 0.0 2.7785634937879655E-4 0.0 20.89793725003348 3.889988891303152E-4 58-59 0.0 2.7785634937879655E-4 0.0 21.712084139348292 3.889988891303152E-4 60-61 0.0 2.7785634937879655E-4 0.0 22.46224071140117 3.889988891303152E-4 62-63 0.0 2.7785634937879655E-4 0.0 23.12987394768854 3.889988891303152E-4 64-65 0.0 2.7785634937879655E-4 0.0 23.76246949831925 4.167845240681949E-4 66-67 0.0 2.7785634937879655E-4 0.0 24.476893743842005 4.445701590060745E-4 68-69 0.0 2.7785634937879655E-4 0.0 25.301599174433214 4.445701590060745E-4 70-71 0.0 2.7785634937879655E-4 0.0 26.098685683896168 4.445701590060745E-4 72-73 0.0 2.7785634937879655E-4 0.0 26.68832464291291 4.445701590060745E-4 74-75 0.0 2.7785634937879655E-4 0.0 27.357930659280875 4.445701590060745E-4 76-77 0.0 2.7785634937879655E-4 0.0 27.877522032619225 4.7235579394395413E-4 78-79 0.0 2.7785634937879655E-4 0.0 28.687778933042733 5.001414288818338E-4 80-81 0.0 2.7785634937879655E-4 0.0 29.37450090053243 5.001414288818338E-4 82-83 0.0 2.7785634937879655E-4 0.0 29.904706386417047 5.001414288818338E-4 84-85 0.0 2.7785634937879655E-4 0.0 30.466392996686288 5.001414288818338E-4 86-87 0.0 2.7785634937879655E-4 0.0 30.954669959449646 5.001414288818338E-4 88 0.0 2.7785634937879655E-4 0.0 31.387208938527618 5.001414288818338E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCCA 1205 0.0 59.71284 1 ACCCATG 1505 0.0 46.872498 3 TACCCAT 1705 0.0 42.47639 2 GTACCTA 215 6.548362E-11 26.248598 1 TCATTAA 1535 0.0 26.021704 94 CATTAAA 1230 0.0 25.597342 94 ATCATTA 1920 0.0 24.71982 94 GTATCAT 3140 0.0 23.79538 94 GTACAGG 915 0.0 23.642862 1 TATCACG 200 2.285924E-8 23.513714 2 AGGGAAG 640 0.0 23.512407 5 ATAAGCA 1450 0.0 22.701633 5 TATATGG 625 0.0 22.573168 2 TACAGGG 865 0.0 22.290459 2 GTATATG 685 0.0 21.96963 1 CAGGGAA 660 0.0 21.3767 4 GAGTACT 16450 0.0 21.14108 12-13 TATCATT 2430 0.0 21.078773 94 ACAGGGA 675 0.0 20.90166 3 ACATAAG 1350 0.0 20.90166 3 >>END_MODULE