FastQCFastQC Report
Mon 6 Jun 2016
ERR522996_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522996_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1799491
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT19913411.066129255439455No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT1125996.257269416740622TruSeq Adapter, Index 1 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT616933.428358352445219RNA PCR Primer, Index 1 (95% over 23bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT491912.733606336458476No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT309481.7198196601149993No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT242391.34699201051853TruSeq Adapter, Index 1 (96% over 26bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT204271.1351543297521356No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTG167830.9326526223248686TruSeq Adapter, Index 1 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158230.8793042032441396No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155480.8640221040283058No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC144520.8031159922444736No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA132950.7388200329982201No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC132030.7337074761696502No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC129840.7215373680668589No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT120750.6710230837497937No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG120700.670745227400415No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT120680.6706340848606633No Hit
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC117260.6516287105631537No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC106590.5923341656057185No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA103720.5763852111513755No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA93850.5215363677840011No Hit
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCT93820.5213696539743738No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90980.5055874133296582No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87240.4848037583961243No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC85010.4724113652138299No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA84480.46946608791041466No Hit
CTTTACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC84170.4677433785442661No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC83640.4647981012408509No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA81370.4521834229790535No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG71690.39839043373931854No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT67820.37688435229739964No Hit
ACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGC67040.3725497932470904Illumina PCR Primer Index 5 (95% over 24bp)
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC63410.3523774222821898No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT62530.34748715053312296No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG62500.3473204367234957TruSeq Adapter, Index 1 (96% over 28bp)
CTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC59390.33003777179213456No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG51220.2846360443036392No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC47740.26529724238687497No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA46890.2605736844474354No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG45070.25045971333004724No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG43350.24090145491141662No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT41650.23145433903253754No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG40060.2226185071222918RNA PCR Primer, Index 46 (96% over 28bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG39080.2171725226744674No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT37720.20961482997136413No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG36490.20277956377664574RNA PCR Primer, Index 26 (100% over 29bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG33830.18799760598969376No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33740.18749746456081193No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC31200.17338236201236903No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC29550.16421310248286877No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG25220.14015074262666496No Hit
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT25080.13937274484840434No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG24110.1339823316704557No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC23280.12936991627076766No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA22730.12631349642760092No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTAGGCAT22230.12353493293381294No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA22160.12314593404468265No Hit
GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT22020.12236793626642202No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21730.12075636944002498No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG19140.10636341054220333No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCCTGTC18570.10319584815928504No Hit
CTTATAACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCG18300.10169542387263955No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT18120.10069514101487588No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACA11100.065.251251
GTACCCA7900.064.89191
GGCGTAC301.3705634E-462.6741394
CTTTACA12750.061.602361
CTTAATA2750.058.1483351
ACCCATG10000.050.7646373
ATGTGTA4550.046.4877624
TACCCAT11050.045.9421272
GATGTGT4600.044.960633
GAGATGT4800.043.112431
GTGTATA5000.041.359186
CGGAGAT1400.040.312921
CTTAACA3900.039.796091
GCGAATC9700.037.55080422-23
CTTATTA2800.036.953511
CTTATCA3250.036.1782651
TAAGGCG10750.036.0675518-19
TGTGTAT5750.035.9694985
TATAAGA6000.034.4650279
AGGCGAA11650.033.6855620-21
Produced by FastQC (version 0.11.5)