FastQCFastQC Report
Mon 6 Jun 2016
ERR522995_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522995_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10462068
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT436150.41688698639695326No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT355010.3393306180001889No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211390.20205374310317997No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159950.15288564364139096No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG160300.049.2831882
GTACCTG183950.043.317531
ACCTGGG190050.041.1237263
CCTGGGG177600.030.234914
GTATCAA559550.024.9754811
GGTATCA410300.024.6368391
CATGGGG301750.024.2620164
GTACATG508100.023.986581
ATGGGGG191950.023.8581985
TACATGG517500.023.2441252
TGGGGGG198350.023.1239286
TATAACG8800.022.977822
ACATGGG519400.022.525673
CTGGGGG117150.021.7933675
TCAACGC643550.021.451584
CAACGCA647650.021.314865
ATCAACG653300.021.1191653
TATCAAC655500.021.0551572
AACGCAG666950.020.870646
GAGTACT349800.020.74095712-13
Produced by FastQC (version 0.11.5)