FastQCFastQC Report
Mon 6 Jun 2016
ERR522995_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522995_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10462068
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT394260.3768471013570166No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT347160.3318273213288233No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206550.19742750668414696No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145650.1392172178578843No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT107380.10263745179251368No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG161400.049.9668272
GTACCTG187850.043.4017561
ACCTGGG187050.042.3327983
CCTGGGG172950.031.3016134
GTATCAA559000.024.2326451
TATAACG9950.024.0887952
GGTATCA416800.023.8589881
TGGGGGG176900.023.1644176
CATGGGG297900.022.5737614
ATGGGGG182700.022.5319235
CTGGGGG113650.022.3697195
GTACATG498900.022.1568761
TAACGCA12200.021.5706394
TACATGG510350.021.2813842
TCAACGC643950.020.7107124
ACATGGG511100.020.6141073
CAACGCA650150.020.5058825
ATCAACG654850.020.3659823
TATCAAC657250.020.3575212
AACGCAG666900.020.0824586
Produced by FastQC (version 0.11.5)