##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR522995_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10462068
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.24561195740651	33.0	31.0	34.0	30.0	34.0
2	32.38024614254084	34.0	31.0	34.0	30.0	34.0
3	32.571501733691655	34.0	31.0	34.0	30.0	35.0
4	35.66464115889899	37.0	35.0	37.0	33.0	37.0
5	35.59563950454155	36.0	35.0	37.0	33.0	37.0
6	35.716633843328104	37.0	35.0	37.0	33.0	37.0
7	35.56147580000436	37.0	35.0	37.0	33.0	37.0
8	35.54825604268678	36.0	35.0	37.0	33.0	37.0
9	36.8463862020396	38.0	37.0	39.0	33.0	39.0
10-11	36.65124352087943	37.5	35.0	39.0	33.0	39.0
12-13	36.652416329161696	37.5	35.0	39.0	33.0	39.0
14-15	37.83471704638127	38.0	37.0	41.0	33.0	41.0
16-17	37.64903023952817	38.0	36.5	41.0	33.0	41.0
18-19	37.5568472217921	38.0	36.0	41.0	33.0	41.0
20-21	37.57557611936761	38.0	36.5	41.0	33.0	41.0
22-23	37.43192626926149	38.0	36.0	41.0	33.0	41.0
24-25	37.355961555593026	38.0	36.5	41.0	32.5	41.0
26-27	37.044961617531065	38.0	36.0	40.0	32.5	41.0
28-29	37.01345345872346	38.0	36.0	40.0	32.0	41.0
30-31	36.77008732881492	38.0	35.5	40.0	32.0	41.0
32-33	36.72559818001565	37.5	36.0	40.0	31.5	41.0
34-35	36.49961723628636	38.0	35.0	40.0	30.5	41.0
36-37	36.28478098211558	37.0	35.5	40.0	30.5	41.0
38-39	36.24246310576456	37.0	35.5	40.0	30.0	41.0
40-41	36.43110726292355	37.5	35.5	40.0	31.0	41.0
42-43	36.474303550693804	37.5	35.5	40.0	31.0	41.0
44-45	36.293095877411616	37.0	35.0	40.0	30.5	41.0
46-47	36.18938234773469	37.0	35.5	40.0	30.0	41.0
48-49	35.97660132776809	38.0	35.0	40.0	30.0	41.0
50-51	35.81398639351226	38.0	34.5	40.0	29.0	41.0
52-53	35.822686011981574	37.5	35.0	40.0	28.5	41.0
54-55	35.62894525250648	37.5	34.5	40.0	28.0	41.0
56-57	35.38437262116821	37.0	35.0	40.0	28.0	41.0
58-59	35.199334682206235	37.0	34.5	40.0	27.5	41.0
60-61	35.01275101633826	37.0	34.0	39.0	27.5	41.0
62-63	34.77107460972343	36.5	34.0	39.0	26.0	41.0
64-65	34.585824188869736	37.0	33.5	39.0	26.0	41.0
66-67	34.085155726382204	36.0	33.5	38.0	26.0	40.0
68-69	33.94634679300498	36.5	33.5	37.5	26.0	40.0
70-71	33.52873490212451	35.0	33.0	37.0	25.5	39.5
72-73	33.294017396942934	35.0	33.0	37.0	25.0	39.0
74-75	33.10591529322883	35.0	32.5	37.0	24.5	39.0
76-77	31.501315370919016	34.0	30.0	36.0	23.0	37.0
78-79	32.6172252942726	35.0	32.0	36.5	24.0	37.0
80-81	32.72526774821192	35.0	32.5	36.0	25.0	37.5
82-83	32.62187155541333	35.0	32.0	37.0	24.5	37.0
84-85	32.2919182899595	35.0	32.0	36.0	24.0	36.5
86-87	32.172784864330836	35.0	32.0	36.0	24.0	36.5
88-89	32.13416821607353	35.0	32.0	36.0	23.0	37.0
90-91	31.898342708152917	35.0	32.0	35.0	23.5	36.5
92-93	31.690197148403165	35.0	31.5	35.0	22.0	36.5
94-95	31.830651024252568	35.0	31.0	35.0	21.5	37.0
96-97	31.55613555560908	35.0	31.0	35.0	21.0	37.0
98-99	31.41233291544272	34.0	31.0	35.0	20.5	36.0
100	30.197750482982904	34.0	29.0	35.0	19.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.0579575246364854
1101	2	-0.1024928233462461
1101	3	-0.10866659987413385
1101	4	-0.08192107458101816
1101	5	-0.1565917957833065
1101	6	-0.07759351536675041
1101	7	-0.11764328270388802
1101	8	-0.19938617627332889
1101	9	-0.16593527625492044
1101	10-11	-0.15981771192713978
1101	12-13	-0.12606874162197101
1101	14-15	-0.2661915816234597
1101	16-17	-0.27540903854088583
1101	18-19	-0.3854076392060932
1101	20-21	-0.33982274822012926
1101	22-23	-0.8168550144800051
1101	24-25	-0.3145537610811431
1101	26-27	-0.2800093031295816
1101	28-29	-0.30094724404114714
1101	30-31	-0.3422632612876839
1101	32-33	-0.3061452441610868
1101	34-35	-0.3569478336222147
1101	36-37	-0.4117902504194788
1101	38-39	-0.33638207453537916
1101	40-41	-0.32983599840382993
1101	42-43	-0.30281760793691603
1101	44-45	-0.3808872894086974
1101	46-47	-0.289419134759882
1101	48-49	-0.4122714450048761
1101	50-51	-0.3245925513617962
1101	52-53	-0.3923338070039364
1101	54-55	-0.32310558224786945
1101	56-57	-0.41781486649529853
1101	58-59	-0.39100572152204904
1101	60-61	-0.5897323863629325
1101	62-63	-0.5138124687896877
1101	64-65	-0.4959103745117659
1101	66-67	-0.42424265317865206
1101	68-69	-0.4511203639442698
1101	70-71	-0.5480122383883739
1101	72-73	-0.5294712952827396
1101	74-75	-0.4752314480095876
1101	76-77	-0.5660871365194389
1101	78-79	-0.5444805160571988
1101	80-81	-0.4233733319368511
1101	82-83	-0.3123192278997706
1101	84-85	-0.39210162275017524
1101	86-87	-0.4494134415238733
1101	88-89	-0.44042001535888886
1101	90-91	-0.4825388874758758
1101	92-93	-0.4126811168577227
1101	94-95	-0.5035069888805772
1101	96-97	-0.6295865656541793
1101	98-99	-0.5856519506649924
1101	100	-0.4704009820601378
1102	1	-0.03230581232993757
1102	2	-0.10749783250717826
1102	3	-0.105668344776916
1102	4	-0.05527152737518293
1102	5	-0.10918302276125047
1102	6	-0.16274679878097942
1102	7	-0.07444734147598098
1102	8	-0.1673851497236143
1102	9	-0.13609749659929804
1102	10-11	-0.1611696736489634
1102	12-13	-0.10265464120085
1102	14-15	-0.2222022657736531
1102	16-17	-0.20817945598899001
1102	18-19	-0.18341705774177086
1102	20-21	-0.22644597126180344
1102	22-23	-0.18437929558623978
1102	24-25	-0.1575350267420177
1102	26-27	-0.19817150879792678
1102	28-29	-0.22857830871763696
1102	30-31	-0.2002900071946172
1102	32-33	-0.20904138498300995
1102	34-35	-0.24854670410856272
1102	36-37	-0.27111769814964504
1102	38-39	-0.3104351036680768
1102	40-41	-0.2600343739217408
1102	42-43	-0.3453513512206996
1102	44-45	-0.1559672930854319
1102	46-47	-0.13185621900338873
1102	48-49	-0.23736325709298
1102	50-51	-0.15729345077074441
1102	52-53	-0.21117419011012828
1102	54-55	-0.21303133698204846
1102	56-57	-0.24655786255285506
1102	58-59	-0.28907032000130783
1102	60-61	-0.26141788211923966
1102	62-63	-0.3501033956041866
1102	64-65	-0.2864123951694353
1102	66-67	-0.39474531880924246
1102	68-69	-0.2984743818552005
1102	70-71	-0.3104985665073343
1102	72-73	-0.353771219662562
1102	74-75	-0.4208926437353071
1102	76-77	-0.3891037096199774
1102	78-79	-0.39068300627059926
1102	80-81	-0.34798219641375994
1102	82-83	-0.300864962659098
1102	84-85	-0.42722874034689085
1102	86-87	-0.43737012676472986
1102	88-89	-0.41943908492945425
1102	90-91	-0.502276748467601
1102	92-93	-0.4376944508038427
1102	94-95	-0.5685608306019034
1102	96-97	-0.597477293724328
1102	98-99	-0.6425312627144777
1102	100	-0.6838942017340521
1103	1	-0.07311527474931978
1103	2	-0.11463862786284551
1103	3	-0.2378350813086385
1103	4	-0.06953460570053238
1103	5	-0.1543330438002144
1103	6	-0.1762626878109046
1103	7	-0.14378061670320363
1103	8	-0.13326410112486542
1103	9	-0.10496051749652224
1103	10-11	-0.2078807436903034
1103	12-13	-0.18107489588043535
1103	14-15	-0.20829398169544788
1103	16-17	-0.25245671337184916
1103	18-19	-0.3215550418366817
1103	20-21	-0.30245119382040997
1103	22-23	-0.3553844220075604
1103	24-25	-0.2457054293617773
1103	26-27	-0.2546364312429503
1103	28-29	-0.2384783434224076
1103	30-31	-0.43811318737145655
1103	32-33	-0.31975333877227996
1103	34-35	-0.3789357252149088
1103	36-37	-0.4412888535005024
1103	38-39	-0.35272647066715024
1103	40-41	-0.3969062260215992
1103	42-43	-0.38301148335941093
1103	44-45	-0.28330897087013085
1103	46-47	-0.43090648394502296
1103	48-49	-0.4032328946983341
1103	50-51	-0.3446358571330208
1103	52-53	-0.34290589550290207
1103	54-55	-1.0896469995093625
1103	56-57	-0.40827057566776404
1103	58-59	-0.43334892410066317
1103	60-61	-0.43628178827399466
1103	62-63	-0.3539082470887678
1103	64-65	-0.3872107080475118
1103	66-67	-0.39979850497361014
1103	68-69	-0.349559420208422
1103	70-71	-0.4689419862996047
1103	72-73	-0.44520279160725096
1103	74-75	-0.5726684761411676
1103	76-77	-0.42636707683388764
1103	78-79	-0.32793927640778975
1103	80-81	-1.1248078553434624
1103	82-83	-0.32616784873212623
1103	84-85	-0.24827605991230683
1103	86-87	-0.2879572454969761
1103	88-89	-0.2588203008801706
1103	90-91	-0.28297381011496014
1103	92-93	-0.25586127687830995
1103	94-95	-0.29154659163735275
1103	96-97	-0.4482530719437179
1103	98-99	-0.34979812106246655
1103	100	-0.41009068981525587
1104	1	-0.08923165331515293
1104	2	-0.20426376188480333
1104	3	-0.13787838044565603
1104	4	-0.034231219254415635
1104	5	-0.06463973196110118
1104	6	-0.20887754496040145
1104	7	-0.20835185498522435
1104	8	-0.14499165371686473
1104	9	-0.20540524983359632
1104	10-11	-0.17796841787390605
1104	12-13	-0.15560574468319288
1104	14-15	-0.2774471357630688
1104	16-17	-0.2767249047139373
1104	18-19	-0.25688208999580553
1104	20-21	-0.3560973172062134
1104	22-23	-0.241776841906578
1104	24-25	-0.19455346626243397
1104	26-27	-0.2415878470113313
1104	28-29	-0.38279169807386637
1104	30-31	-0.4102980900369104
1104	32-33	-0.30069501133593235
1104	34-35	-0.33052169612856375
1104	36-37	-0.39786760489500494
1104	38-39	-0.31791284434160616
1104	40-41	-0.17662542745330967
1104	42-43	-0.2360130567042944
1104	44-45	-0.35117704914490133
1104	46-47	-0.32081407188715616
1104	48-49	-0.3398988487016723
1104	50-51	-0.23602043520015314
1104	52-53	-0.3287870025170463
1104	54-55	-0.3704371320080355
1104	56-57	-0.3669155781991904
1104	58-59	-0.3688134688007594
1104	60-61	-0.42656406379784073
1104	62-63	-0.33607176155705787
1104	64-65	-0.5614045814201489
1104	66-67	-0.4277933123961617
1104	68-69	-0.37009214480004715
1104	70-71	-0.3209583002970362
1104	72-73	-0.37814434219761495
1104	74-75	-0.4384269764836475
1104	76-77	-0.3684481542259448
1104	78-79	-0.3043814550499846
1104	80-81	-0.3757907637317004
1104	82-83	-0.37115355697449104
1104	84-85	-1.0348742717174133
1104	86-87	-0.498786462001096
1104	88-89	-0.3645248204333029
1104	90-91	-0.4428245504804167
1104	92-93	-0.35031773579992986
1104	94-95	-0.35612989587180266
1104	96-97	-0.341254107333814
1104	98-99	-0.36665145619543793
1104	100	-1.464823232940489
1105	1	-0.0575017139213756
1105	2	-0.15645957041451908
1105	3	-0.16266338412834358
1105	4	-0.06399188306841097
1105	5	-0.11765610544615868
1105	6	-0.17186715747344294
1105	7	-0.11500020135660094
1105	8	-0.17231856490771236
1105	9	-0.12268416394783799
1105	10-11	-0.16391454054470245
1105	12-13	-0.12222001825013251
1105	14-15	-0.23677982815378584
1105	16-17	-0.27128312781484
1105	18-19	-0.2563481421785312
1105	20-21	-0.26822756352963495
1105	22-23	-0.27190135722578646
1105	24-25	-0.13006491137165455
1105	26-27	-0.263207914828989
1105	28-29	-0.2625054958864794
1105	30-31	-0.2952004109831847
1105	32-33	-0.18944750564124035
1105	34-35	-0.20684728784320328
1105	36-37	-0.19997665861672687
1105	38-39	-0.249582247786428
1105	40-41	-0.1811746588408525
1105	42-43	-0.19628671667295805
1105	44-45	-0.3591388147867818
1105	46-47	-0.2939635843033841
1105	48-49	-0.3390456565765021
1105	50-51	-0.16236672425278442
1105	52-53	-0.28710463855644974
1105	54-55	-1.1703858320579315
1105	56-57	-0.32985318802308683
1105	58-59	-0.26283981883775454
1105	60-61	-0.2626273598380422
1105	62-63	-0.2131652127988488
1105	64-65	-0.1635058380848733
1105	66-67	-0.16742254058456751
1105	68-69	-0.009298692186249014
1105	70-71	-0.06262630830291016
1105	72-73	-0.1510994301454076
1105	74-75	-0.22180381828739115
1105	76-77	-0.2200421838888964
1105	78-79	-0.14166738316915684
1105	80-81	-0.0832444155820653
1105	82-83	-0.08595240461631448
1105	84-85	-0.09223263549998251
1105	86-87	-0.07891129522606377
1105	88-89	-0.1916055052491572
1105	90-91	-0.18498641671437355
1105	92-93	-0.1268160005364507
1105	94-95	-0.17013039849753753
1105	96-97	-0.1929447803469948
1105	98-99	-0.07406444957262082
1105	100	-0.11596576950048743
1106	1	-0.025659925345458134
1106	2	-0.0979657025435543
1106	3	-0.11171792145147208
1106	4	-0.05153339097266496
1106	5	-0.11119098047999643
1106	6	-0.08693549730416805
1106	7	-0.0704236093779258
1106	8	-0.1643753959644556
1106	9	-0.19030992231744648
1106	10-11	-0.20251005366878871
1106	12-13	-0.1874857749315595
1106	14-15	-0.20049989705206883
1106	16-17	-0.16449188555417749
1106	18-19	-0.24177352518447748
1106	20-21	-0.2330346900762521
1106	22-23	-0.13471475195851923
1106	24-25	-0.10255286268303365
1106	26-27	-0.10145450623172536
1106	28-29	-0.12380939145618441
1106	30-31	-0.1409470705893341
1106	32-33	-0.0906180629343325
1106	34-35	-0.12034893090449117
1106	36-37	-0.20380607684035112
1106	38-39	-0.20934047889040386
1106	40-41	-0.1765007539838095
1106	42-43	-0.14303504557248914
1106	44-45	-0.1410073834079384
1106	46-47	-0.3336410718804501
1106	48-49	-0.2600944690091609
1106	50-51	-0.18567266196125587
1106	52-53	-0.25386065753775
1106	54-55	-0.32484944885401745
1106	56-57	-0.16645928305411672
1106	58-59	-0.20782675059539457
1106	60-61	-0.17037811351502086
1106	62-63	-0.22900729424142696
1106	64-65	-0.17987214153625075
1106	66-67	-0.14684404030907672
1106	68-69	-0.08889409315358421
1106	70-71	-0.12049746142577078
1106	72-73	-0.12801032103136123
1106	74-75	-0.17910395135984203
1106	76-77	-0.16409065985132543
1106	78-79	-0.07228130025850987
1106	80-81	-0.0943805074116213
1106	82-83	-0.1613208022042656
1106	84-85	-0.08766815924747817
1106	86-87	-0.002636503281479463
1106	88-89	-0.1277248959838886
1106	90-91	-0.0775425667861569
1106	92-93	-0.06082085195675191
1106	94-95	-0.07894064864438022
1106	96-97	0.005923439212534021
1106	98-99	-0.10873860392594636
1106	100	-0.07419584751354691
1107	1	-0.037746922665974125
1107	2	-0.05078653128417443
1107	3	-0.09107205650784778
1107	4	-0.006912016411810384
1107	5	-0.10198060355764227
1107	6	-0.11492528096617605
1107	7	-0.14902428543302193
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2308	88-89	0.15835620700325137
2308	90-91	0.17585124386219064
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2308	94-95	0.15628386133068872
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2309	3	0.11175211956890507
2309	4	0.11801517964392616
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2312	78-79	0.22175995674536608
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2312	84-85	0.19891415921185995
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2312	92-93	0.3558606543851077
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2313	92-93	0.11637972196595925
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2313	96-97	0.07308229552603152
2313	98-99	0.22934657438300476
2313	100	0.22909539870272866
2314	1	-1.969692680346089
2314	2	-0.007217425718998527
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2314	4	-0.1680284067865898
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2314	28-29	0.23347168037049215
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2314	64-65	0.3841073136967452
2314	66-67	0.408467713551957
2314	68-69	0.43100300522696955
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2314	74-75	0.5192001631048768
2314	76-77	0.5580923905381105
2314	78-79	0.3985919234529547
2314	80-81	0.3422174811355134
2314	82-83	0.3099702148825898
2314	84-85	0.3142858692584696
2314	86-87	0.31774505763012684
2314	88-89	0.24549792655703584
2314	90-91	0.26829896685332244
2314	92-93	0.10988223206949854
2314	94-95	-0.042128785445928685
2314	96-97	0.052239479455813864
2314	98-99	0.14360752810742738
2314	100	0.12618461113468626
2315	1	0.05212450003391922
2315	2	-0.02277719786022203
2315	3	0.02079407811481815
2315	4	-0.07028156368897243
2315	5	-0.009536502158603355
2315	6	0.014919183543185
2315	7	0.041140829438646165
2315	8	0.04351562983791979
2315	9	0.06478165646211664
2315	10-11	0.05491864112304512
2315	12-13	0.11282091829969687
2315	14-15	0.09597939115115395
2315	16-17	0.13703990256674814
2315	18-19	0.1519985756706106
2315	20-21	0.1371479124404189
2315	22-23	0.17153360630977232
2315	24-25	0.23708717291712844
2315	26-27	0.1382241241816331
2315	28-29	-1.16137266468359
2315	30-31	0.19646763196921313
2315	32-33	-1.4083835523301218
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2315	64-65	0.39978955362798274
2315	66-67	0.2772861726529783
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2315	82-83	0.16048939779818028
2315	84-85	0.13823096113929267
2315	86-87	0.19439034076643225
2315	88-89	0.26141731170396554
2315	90-91	0.2864620258675359
2315	92-93	0.3081962259253892
2315	94-95	0.3044464786300871
2315	96-97	0.23214016006038207
2315	98-99	0.2970260929208308
2315	100	0.16658299323508885
2316	1	0.019462396882182986
2316	2	-0.09834862533201516
2316	3	-0.11820818074968997
2316	4	-0.008233562429296626
2316	5	-0.06627605815574356
2316	6	-0.030124290535148646
2316	7	-0.0035925288695466406
2316	8	0.02959247829338807
2316	9	-0.043813678552133695
2316	10-11	0.011657551012426381
2316	12-13	0.07053555858698246
2316	14-15	0.05542605888429364
2316	16-17	-1.27948310283778
2316	18-19	0.06578089566568934
2316	20-21	-0.07069228213853052
2316	22-23	0.050350979445418886
2316	24-25	0.12948194220421527
2316	26-27	-0.03131543669721282
2316	28-29	0.04474399358168313
2316	30-31	0.0705589302657188
2316	32-33	0.22889636671656888
2316	34-35	0.19157300186213888
2316	36-37	0.19159287540725956
2316	38-39	0.18370313144013295
2316	40-41	0.07224960372102629
2316	42-43	0.0727673354007834
2316	44-45	0.15535256469163272
2316	46-47	0.22288897008926511
2316	48-49	0.18565028189365762
2316	50-51	0.20030926447321917
2316	52-53	0.19199253374059566
2316	54-55	0.2577216578216692
2316	56-57	0.3160489642445512
2316	58-59	0.26907325304684804
2316	60-61	-1.1427297398386926
2316	62-63	0.18647834755324055
2316	64-65	0.296396486834837
2316	66-67	0.0933877163838801
2316	68-69	0.298818164306212
2316	70-71	0.26734446231454
2316	72-73	0.4308325474440622
2316	74-75	0.24832356859838
2316	76-77	0.010763978104314731
2316	78-79	-0.051295075005860724
2316	80-81	0.07763061401281135
2316	82-83	0.025995447540424266
2316	84-85	-0.5611665314122192
2316	86-87	0.04614758436225941
2316	88-89	0.15704273144966407
2316	90-91	0.21204047477578314
2316	92-93	0.4010582707120669
2316	94-95	0.2664855101592245
2316	96-97	0.3002369064810715
2316	98-99	0.37250052845098125
2316	100	0.15336725789486394
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	3.0
9	2.0
10	3.0
11	9.0
12	60.0
13	337.0
14	1234.0
15	3409.0
16	7369.0
17	13888.0
18	22839.0
19	33153.0
20	44268.0
21	59096.0
22	75509.0
23	88691.0
24	98370.0
25	109581.0
26	126034.0
27	148799.0
28	179874.0
29	218313.0
30	268377.0
31	331449.0
32	414403.0
33	538110.0
34	767447.0
35	1364950.0
36	2143426.0
37	1573327.0
38	1525258.0
39	304297.0
40	181.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.07233077650729	18.17673427280402	13.088133422636727	23.662801528051965
2	16.48856898519309	20.5345205577403	39.044373318385006	23.932537138681603
3	19.383366653705558	25.89380990450454	29.558678073971606	25.164145367818293
4	13.543033612077487	16.453354282715143	36.18817089549336	33.81544120971401
5	13.96219471746879	36.6427643583511	33.48758319370141	15.907457730478693
6	31.198774467915904	35.03123856583613	18.73235769448258	15.03762927176539
7	28.218586355156372	32.152046126279885	21.553533137157856	18.075834381405883
8	27.023012004995955	33.97761683859423	20.19373620551438	18.80563495089543
9	27.631821930425225	15.834278653130529	20.046877921267573	36.48702149517667
10-11	25.6802921602255	25.458490876683793	27.83771945650322	21.02349750658749
12-13	25.99011091054465	23.26414985229929	27.931396712016355	22.814342525139708
14-15	23.51967873797128	24.63600941596461	26.119885635291062	25.72442621077305
16-17	22.945732195762627	27.32086652266138	26.683849358115165	23.049551923460825
18-19	22.79774075151335	26.45714329955765	28.525468638451134	22.219647310477868
20-21	23.519921651478406	25.73550310283296	28.400693884513068	22.34388136117557
22-23	23.280632452625458	25.43674484450919	28.3757332846747	22.90688941819065
24-25	23.06838634366501	25.455865783906194	28.351824968644785	23.12392290378401
26-27	22.960977936544484	25.50602147547336	28.460472862694907	23.07252772528724
28-29	22.93117653432866	26.012273357840794	28.437333886009014	22.619216221821535
30-31	23.65429861182308	25.584425423040624	28.378234536824674	22.38304142831162
32-33	23.431426056173944	25.670854112479898	28.27222060488699	22.625499226459173
34-35	23.413310962182532	25.86548325930027	28.219171574057466	22.502034204459726
36-37	23.43233395939081	25.830508332003454	28.106482249509263	22.630675459096473
38-39	23.31342230265165	26.042631154262274	27.944100252527775	22.699846290558305
40-41	23.30051534258393	25.864682245235205	28.136940372963217	22.697862039217654
42-43	23.10913503425679	25.993541086187054	27.773119097891367	23.124204781664787
44-45	22.915180930680076	26.053518948507808	27.90519864886586	23.126101471946253
46-47	22.98833669155065	25.913723657703315	27.753890358610196	23.344049292135843
48-49	23.0935358919407	26.030071655059555	27.70657682119791	23.169815631801836
50-51	22.963098184117452	26.315189708295627	27.600218752349676	23.12149335523725
52-53	23.05485870979136	26.452658740429474	27.184632382094055	23.30785016768511
54-55	22.86825117665395	26.17837690530286	27.590036645246542	23.36333527279665
56-57	23.10237941342736	26.225561464755	27.21328290918434	23.458776212633296
58-59	23.08031148856409	26.226910176987005	27.428306526129354	23.264471808319552
60-61	23.35367781694247	26.28229796696631	27.501294231855233	22.862729984235987
62-63	23.093164558292937	26.4254743512327	27.436012197415373	23.045348893058986
64-65	22.986010519268255	26.181778419016677	27.29188223768415	23.54032882403092
66-67	22.929621029696374	26.192100099718335	27.191797336399677	23.68648153418561
68-69	22.634673412056202	26.283658585577722	27.359240310189403	23.72242769217667
70-71	22.791615009733036	26.126343384947013	27.409581189693057	23.672460415626897
72-73	22.72711175124199	26.507081822213387	26.987794919004703	23.77801150753992
74-75	22.788026619874174	26.64681579814457	26.813569976103825	23.751587605877432
76-77	22.681409792928513	26.186009703381195	27.119809113561832	24.01277139012846
78-79	22.57854765312309	26.235808682556815	27.21938954125384	23.966254123066257
80-81	22.764350851677232	26.45104760419122	26.784968393419962	23.999633150711592
82-83	22.813288794249843	26.469692976157223	26.848727618047167	23.868290611545767
84-85	22.686396354666787	26.67581657639423	26.660016589508313	23.977770479430674
86-87	22.578039269550555	26.578632080244123	26.824268094573856	24.019060555631466
88-89	22.33252727692774	26.410663228716142	26.90937538323045	24.347434111125672
90-91	22.846640794048877	26.182008150237824	26.883621776508683	24.08772927920462
92-93	22.659807697739428	26.348569700142498	26.80856215188664	24.18306045023143
94-95	22.52952560175862	26.180239091433627	26.771914854854888	24.518320451952864
96-97	22.273030039055662	26.156191007462485	26.96016823389065	24.610610719591207
98-99	22.298258075983103	26.12474059153088	26.784178032098666	24.792823300387347
100	22.195114724169738	26.131675914376245	26.96044274305705	24.712766618396966
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5186.0
1	3897.0
2	6826.5
3	9353.0
4	5594.5
5	3877.0
6	5368.0
7	9423.5
8	14521.0
9	16897.0
10	14478.0
11	11216.0
12	11000.0
13	11886.5
14	12772.0
15	13650.0
16	14443.5
17	15368.5
18	16475.0
19	17530.0
20	18684.5
21	20289.0
22	22499.0
23	25816.5
24	30296.5
25	35949.0
26	43815.0
27	52836.5
28	62753.5
29	75194.5
30	89238.5
31	102638.5
32	117748.0
33	134724.0
34	150377.5
35	166835.5
36	190017.0
37	213668.0
38	230743.0
39	246345.0
40	258944.0
41	271572.0
42	285758.5
43	301916.5
44	321717.5
45	341427.5
46	362731.5
47	390327.0
48	425372.0
49	447549.5
50	462533.0
51	460479.0
52	436691.0
53	422973.0
54	411706.0
55	387627.5
56	354407.5
57	323470.0
58	292341.5
59	254117.5
60	212757.0
61	175429.5
62	143347.5
63	112316.0
64	84605.0
65	64312.5
66	48230.0
67	37416.0
68	29214.0
69	21846.5
70	17151.0
71	13453.0
72	10504.5
73	8462.0
74	6746.0
75	5067.5
76	3588.5
77	2320.0
78	1433.5
79	892.5
80	560.5
81	353.0
82	252.5
83	170.5
84	104.0
85	74.0
86	48.0
87	38.0
88	23.0
89	12.0
90	12.5
91	10.0
92	5.5
93	4.0
94	3.0
95	2.0
96	1.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04618589747266028
2	0.01091562394738784
3	0.0
4	2.3895849271864797E-4
5	3.6321690893234494E-4
6	0.0
7	5.735003825247551E-5
8	6.690837796122143E-5
9	0.0
10-11	4.77916985437296E-6
12-13	3.154252103886153E-4
14-15	0.012650462604525223
16-17	0.0017539553365548764
18-19	4.779169854372959E-5
20-21	9.223797818939812E-4
22-23	6.404087604859766E-4
24-25	0.004693144796994246
26-27	0.016406890110062373
28-29	0.01378790502986599
30-31	0.009993244165493858
32-33	0.00725000066908378
34-35	0.009328939555736018
36-37	0.005433916124422055
38-39	0.004267798679955053
40-41	0.005548616200927006
42-43	0.006800758702772721
44-45	0.007479400822093682
46-47	0.015838168897391987
48-49	0.01802224952084043
50-51	0.017563449214820625
52-53	0.011857120408699313
54-55	0.003928477620294573
56-57	0.003957152639420811
58-59	0.010265656847193117
60-61	0.0025234016831089225
62-63	1.4815426548556173E-4
64-65	2.724126816992587E-4
66-67	9.60613140728965E-4
68-69	8.124588752434032E-5
70-71	1.5771260519430766E-4
72-73	0.003044331197235575
74-75	0.005362228576606461
76-77	0.005352670236897715
78-79	0.003302406369371715
80-81	5.735003825247551E-5
82-83	9.128214421852352E-4
84-85	3.2498355009736126E-4
86-87	4.1578777733044744E-4
88-89	2.007251338836643E-4
90-91	4.014502677673286E-4
92-93	4.635794758741771E-4
94-95	3.345418898061072E-5
96-97	0.0019546804704385404
98-99	4.2534611703919344E-4
100	1.7205011475742652E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.0462068E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.469488073019775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.7893053237481	32.17923620543956
2	17.11096699281626	16.24497687166653
3	6.479712126860411	9.227658525678056
4	3.0107447341591533	5.716740449963
5	1.5633072314566079	3.710469698904751
6	0.9411475101569079	2.6805474305007357
7	0.6306545857787723	2.095579523647446
8	0.4614749205617474	1.7524782590082895
9	0.31332836422066246	1.338618334348038
>10	1.5493451331832069	12.937176623709098
>50	0.09373632754722937	3.0513748349367873
>100	0.0513318297299183	4.500472535722284
>500	0.0031247622406995064	1.036821641434506
>1k	0.0014864619934442478	1.1966093577072465
>5k	1.8201575278962417E-4	0.6017607536695754
>10k+	1.5167979399135348E-4	1.7294789536641142
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39426	0.3768471013570166	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34716	0.3318273213288233	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20655	0.19742750668414696	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14565	0.1392172178578843	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	10738	0.10263745179251368	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.911667941749184E-5
2	0.0	0.0	0.0	0.0	2.8675019126237756E-5
3	0.0	0.0	0.0	0.0	2.8675019126237756E-5
4	0.0	0.0	0.0	0.0	5.735003825247551E-5
5	0.0	0.0	0.0	0.0	5.735003825247551E-5
6	0.0	0.0	0.0	0.0	1.2425841621369695E-4
7	0.0	0.0	0.0	0.0	1.2425841621369695E-4
8	0.0	0.0	0.0	0.0	1.3381675592244287E-4
9	0.0	0.0	0.0	9.55833970874592E-6	2.102834735924102E-4
10-11	0.0	0.0	0.0	1.4337509563118878E-5	2.1984181330115613E-4
12-13	4.77916985437296E-6	0.0	0.0	1.911667941749184E-5	3.2498355009736126E-4
14-15	2.8675019126237756E-5	0.0	0.0	2.3895849271864796E-5	3.871127582042097E-4
16-17	2.8675019126237756E-5	0.0	0.0	7.646671766996735E-5	4.110086074760745E-4
18-19	2.8675019126237756E-5	0.0	0.0	8.602505737871328E-5	4.3490445674793934E-4
20-21	2.8675019126237756E-5	0.0	0.0	1.1470007650495102E-4	4.77916985437296E-4
22-23	2.8675019126237756E-5	0.0	0.0	3.1064604053424234E-4	5.973962317966199E-4
24-25	2.8675019126237756E-5	0.0	0.0	0.0010131840091270675	7.07317138447198E-4
26-27	2.8675019126237756E-5	0.0	0.0	0.002136288924904713	7.551088369909276E-4
28-29	2.8675019126237756E-5	0.0	0.0	0.005424357784713309	7.742255164084194E-4
30-31	2.8675019126237756E-5	0.0	0.0	0.014944464134624243	7.885630259715383E-4
32-33	2.8675019126237756E-5	0.0	0.0	0.0373635499214878	8.22017214952149E-4
34-35	2.8675019126237756E-5	0.0	0.0	0.07276764020268268	8.459130642240139E-4
36-37	2.8675019126237756E-5	0.0	0.0	0.12700165971010702	8.602505737871327E-4
38-39	2.8675019126237756E-5	0.0	0.0	0.2056190038145422	9.176006120396082E-4
40-41	3.345418898061072E-5	0.0	0.0	0.30086785901219526	9.558339708745919E-4
42-43	3.823335883498368E-5	0.0	0.0	0.39332567901489457	0.0010131840091270675
44-45	3.823335883498368E-5	0.0	0.0	0.5040638237105705	0.0010323006885445594
46-47	3.823335883498368E-5	0.0	0.0	0.6332925765728152	0.00106575487752517
48-49	3.823335883498368E-5	0.0	0.0	0.7677640787653073	0.0011278840856320184
50-51	3.823335883498368E-5	0.0	0.0	0.9149911852991206	0.0011470007650495103
52-53	3.823335883498368E-5	0.0	0.0	1.065960381828908	0.0012664800114088343
54-55	3.823335883498368E-5	0.0	0.0	1.24315288334964	0.0013811800879137853
56-57	3.823335883498368E-5	0.0	0.0	1.4387834221685427	0.0014433092960206336
58-59	3.823335883498368E-5	0.0	0.0	1.6632323552093142	0.0014767634850012443
60-61	3.823335883498368E-5	0.0	0.0	1.8914663907747493	0.0015197760136906012
62-63	3.823335883498368E-5	0.0	0.0	2.127590835769754	0.0015388926931080929
64-65	3.823335883498368E-5	0.0	0.0	2.366071411502965	0.0015723468820887038
66-67	3.823335883498368E-5	0.0	0.0	2.63999909004606	0.0016105802409236874
68-69	3.823335883498368E-5	0.0	0.0	2.9556011297192866	0.001648813599758671
70-71	3.823335883498368E-5	0.0	0.0	3.2946449975282133	0.0016918261284480278
72-73	6.690837796122143E-5	0.0	0.0	3.6111789753230434	0.0019212262814579297
74-75	7.646671766996735E-5	0.0	0.0	3.935565129188608	0.0019546804704385404
76-77	7.646671766996735E-5	0.0	0.0	4.2980938376619235	0.0019737971498560323
78-79	7.646671766996735E-5	0.0	0.0	4.703620737315032	0.0019785763197104054
80-81	7.646671766996735E-5	0.0	0.0	5.115685541328922	0.002026368018254135
82-83	7.646671766996735E-5	0.0	0.0	5.540353016248796	0.002102834735924102
84-85	7.646671766996735E-5	0.0	0.0	5.973403155093238	0.002107613905778475
86-87	7.646671766996735E-5	0.0	0.0	6.42059485753677	0.002136288924904713
88	7.646671766996735E-5	0.0	0.0	6.771825608474347	0.0021697431138853235
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	16140	0.0	49.966827	2
GTACCTG	18785	0.0	43.401756	1
ACCTGGG	18705	0.0	42.332798	3
CCTGGGG	17295	0.0	31.301613	4
GTATCAA	55900	0.0	24.232645	1
TATAACG	995	0.0	24.088795	2
GGTATCA	41680	0.0	23.858988	1
TGGGGGG	17690	0.0	23.164417	6
CATGGGG	29790	0.0	22.573761	4
ATGGGGG	18270	0.0	22.531923	5
CTGGGGG	11365	0.0	22.369719	5
GTACATG	49890	0.0	22.156876	1
TAACGCA	1220	0.0	21.570639	4
TACATGG	51035	0.0	21.281384	2
TCAACGC	64395	0.0	20.710712	4
ACATGGG	51110	0.0	20.614107	3
CAACGCA	65015	0.0	20.505882	5
ATCAACG	65485	0.0	20.365982	3
TATCAAC	65725	0.0	20.357521	2
AACGCAG	66690	0.0	20.082458	6
>>END_MODULE