FastQCFastQC Report
Mon 6 Jun 2016
ERR522994_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522994_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10310628
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT8516178.25960358573697No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT5439665.275779516048877No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT3561623.4543191743509705No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT2692572.61145101927836No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC2598842.520544820354299No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC2315042.2452948549787655No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA2255662.18770379457003No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC2247062.1793628865283474No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT2183922.118125103533946No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT2024191.963207284755109No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG2015721.9549924602070796No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC1843281.787747555241058No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA1599151.5509724528903575No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC1493031.4480495271481038No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC1472901.4285259830923975No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT1430701.3875973413064655TruSeq Adapter, Index 1 (95% over 21bp)
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA1382921.3412568080237208No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG1171861.1365554067123749No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC1076961.044514456345433No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT1038511.007222838414886No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG921950.8941744382592409No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC831000.8059644863533045No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT779890.756394275886978RNA PCR Primer, Index 1 (95% over 23bp)
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG734350.7122262581871832No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT730050.7080558041663417No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT490150.47538326472451536No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT469830.45567544479346944No Hit
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT445810.4323790946584437No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG435280.4221663316725227No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA425450.4126324798062737No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG398270.3862713309024436No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA378400.3669999538340439No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT364480.35349932128285494No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT321510.31182387726528393No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT295390.28649079377124265TruSeq Adapter, Index 1 (96% over 26bp)
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA290930.2821651600659048No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA282900.2743770796502405No Hit
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA280260.2718166148560495No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259610.2517887368257297No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA251430.24385517545585No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC233160.22613559523241453No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228700.22180996152707674No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG217580.21102497345457522No Hit
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT211050.2046917025810649No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTG208590.20230581493193237TruSeq Adapter, Index 1 (95% over 24bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCCTGTC195950.19004661985671484No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCCTGTC195080.18920283032226554No Hit
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC186940.18130806387350995No Hit
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT174680.1694174205489714No Hit
GCTTATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT172390.16719641131461635No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG165320.16033940900593058No Hit
CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC165190.16021332551227724No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT159420.15461715814012492No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC157540.15279379684729194No Hit
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC153340.14872033012926078No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC147430.14298838053317411No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG146770.14234826433462636No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA142470.13817781031378495No Hit
AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG137180.13304718199512192No Hit
GCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTT135990.13189303309167977No Hit
GCTTGCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT135070.13100074990582533No Hit
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCT120250.116627231629344No Hit
TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA119490.115890128127986No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC119030.11544398653505877No Hit
CTTTACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC112960.10955685725447567No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCCTGTCTCTTATACACATCT111150.10780138707360987No Hit
GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG109060.10577435244487532No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG105150.10198214890499396No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCCTGTCTCTTATACACATCT104280.10113835937054463No Hit
GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT103640.10051764063255894No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTACA19450.059.4893651
GTACCCA32450.054.7899631
TACCCAT38150.045.957222
ACCCATG38350.045.716213
CTATACA17950.045.3320661
GCGTCAG3500.038.97211
GCTTATA18800.037.528161
TATAACG4150.036.244432
CGGAGAT3600.033.9699361
ATAACGC4450.033.8000033
GAGATGT9900.032.7821771
CGTCAGA4550.030.9919742
GCTTATG74500.028.2843111
CTTAATA7000.027.5492421
GGTTCCG605.8370915E-627.42414590-91
CGACGAA1205.843658E-627.41995494
TATAAGA12200.027.350839
CTTAACA8100.027.2920841
AGCGTCA1904.474714E-1027.2123363
GCTTTGC27700.026.9985981
Produced by FastQC (version 0.11.5)