FastQCFastQC Report
Mon 6 Jun 2016
ERR522993_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522993_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5546751
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164410.29640775293500643No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123160.2220398932636421No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84590.1525036908994112No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG99400.056.5424352
ACCTGGG109500.050.2958153
GTACCTG134150.042.281421
CCTGGGG96700.036.136624
TAACGCA7100.031.1332634
ATAACGC7450.030.3021893
GTATCAA207700.028.8939671
GGTATCA144150.027.3088741
TATAACG7750.027.3088742
GTACCCG12250.026.4914651
CTGGGGG64150.026.0246835
TACCCGG12150.025.1611272
GTACATG190050.024.697651
TCAACGC244800.024.264824
TATCACG5050.024.2144722
CAACGCA246650.024.100155
ATCAACG248200.023.9136923
TATCAAC248850.023.9081442
TACATGG193400.023.7105222
AACGCAG256750.023.3783956
Produced by FastQC (version 0.11.5)