FastQCFastQC Report
Mon 6 Jun 2016
ERR522993_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522993_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5546751
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158700.2861134382992855No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131650.23734615092691197No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT113500.20462429267151167No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84800.1528822909122836No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT56310.10151888916592794TruSeq Adapter, Index 11 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG107700.057.6957742
ACCTGGG117750.051.0145763
GTACCTG142750.043.890421
CCTGGGG101950.037.9895674
TATAACG6350.035.530052
TAACGCA6950.032.4624144
CTGGGGG60900.030.7183555
GGTATCA152200.028.8960551
ATAACGC8150.028.2593923
GTATCAA219000.028.1364151
GTACCCG11950.025.5850981
GTACATG190300.024.1983381
TCAACGC252400.023.8925044
TACCGGG8100.023.791792
CAACGCA256450.023.5156045
ATCAACG257800.023.4102733
TATCAAC259500.023.3476852
TATCACG5500.023.0743432
TACATGG193300.022.954532
AACGCAG265150.022.812466
Produced by FastQC (version 0.11.5)