FastQCFastQC Report
Mon 6 Jun 2016
ERR522992_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522992_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7536563
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT575870.7641016203274622No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192170.2549836045953573No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT161570.2143815423555804No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153390.20352778846272498No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC125850.16698593244692572No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC115550.15331922522242566No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA93630.12423434926504297No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG92640.12292075313375607No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90360.11989550143745896No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA79790.1058705407226079No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG255600.028.0871351
TACATGG261950.027.0263042
ACATGGG268850.025.7753833
CATGGGG193750.023.995774
ATGGGGG113550.021.2379825
TATACCG5300.020.4002255
GAGTACT183200.019.3112112-13
AGAGTAC291050.019.19254510-11
TACCTGG55050.019.1280542
GAGTACA199100.018.6781771
GTACTTT196850.017.91228514-15
AGTACAT195200.017.845082
AGTACTT195400.017.0595612-13
GTACCTG65400.016.893491
GTATAGG18950.016.1262511
CATGGGA93150.015.3920714
TATACTG30750.014.8289235
ACTTTTT245550.014.742063516-17
TACTTTT226700.014.6729514-15
GTATAAG23750.014.6486421
Produced by FastQC (version 0.11.5)