##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR522992_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7536563
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.863222134545946	34.0	31.0	34.0	30.0	34.0
2	32.17048182308036	34.0	31.0	34.0	29.0	35.0
3	32.01699567826873	34.0	31.0	34.0	28.0	35.0
4	34.62615332745178	35.0	35.0	37.0	32.0	37.0
5	34.6293039678697	37.0	34.0	37.0	32.0	37.0
6	34.79909767887563	37.0	35.0	37.0	32.0	37.0
7	34.64373388771513	37.0	35.0	37.0	32.0	37.0
8	34.783429661504854	37.0	35.0	37.0	32.0	37.0
9	36.032789084361134	38.0	35.0	39.0	32.0	39.0
10-11	36.12888998605863	38.0	35.0	39.0	32.0	39.0
12-13	36.174217212806425	38.0	35.5	39.0	32.0	39.0
14-15	37.269230350758036	39.0	36.5	41.0	32.0	41.0
16-17	37.15763538366228	39.0	36.5	41.0	32.0	41.0
18-19	37.170910267717524	39.0	36.5	41.0	32.0	41.0
20-21	37.18416332484715	39.0	36.0	41.0	32.0	41.0
22-23	37.09119462014714	39.0	36.0	41.0	32.0	41.0
24-25	37.08935916809824	39.0	36.5	41.0	32.0	41.0
26-27	36.915429553232684	39.0	36.5	41.0	32.0	41.0
28-29	36.71717054577796	39.0	35.0	41.0	31.0	41.0
30-31	36.63621155956635	38.0	36.0	41.0	30.5	41.0
32-33	36.47311559659224	38.0	35.0	41.0	30.0	41.0
34-35	36.3679107439293	38.5	35.0	41.0	30.0	41.0
36-37	36.2491956213993	38.0	35.5	40.5	30.0	41.0
38-39	36.00993927868711	38.0	35.5	40.0	29.0	41.0
40-41	35.95635099182479	38.0	35.0	40.0	29.5	41.0
42-43	35.91093619200158	38.0	35.0	40.0	29.0	41.0
44-45	35.81225613054651	38.0	35.0	40.0	28.5	41.0
46-47	35.533606433065046	38.0	34.5	40.0	27.5	41.0
48-49	35.653328778648834	37.5	35.0	40.0	27.5	41.0
50-51	34.63259731259461	37.5	33.5	39.0	26.0	40.0
52-53	34.794765598058426	37.0	34.5	39.5	26.5	40.0
54-55	35.369712958015484	37.0	34.5	40.0	28.0	41.0
56-57	35.506265455486805	37.0	35.0	40.0	28.0	41.0
58-59	35.48283123222084	38.0	35.0	40.0	27.5	41.0
60-61	35.20692562378899	37.0	35.0	40.0	27.0	41.0
62-63	34.99224354656094	37.0	34.0	40.0	26.5	41.0
64-65	34.75161549104015	36.5	34.0	39.5	26.0	41.0
66-67	34.49898979150045	37.0	34.0	39.0	26.0	41.0
68-69	34.15413338945086	36.0	34.0	38.5	26.0	41.0
70-71	33.747389692091744	36.0	33.5	37.5	25.0	40.0
72-73	33.48796055974056	35.5	33.5	37.0	25.0	39.5
74-75	33.23384406127833	35.0	33.0	37.0	24.0	39.0
76-77	32.90003599784146	35.0	33.0	36.5	24.0	39.0
78-79	32.60112640204825	35.0	33.0	37.0	23.5	37.5
80-81	32.37985106738974	35.0	32.0	36.0	23.0	37.5
82-83	32.166956807765025	35.0	32.0	36.0	22.0	37.0
84-85	31.881209312520838	35.0	32.0	35.5	20.5	37.0
86-87	31.79415524026005	35.0	32.0	36.0	20.0	37.0
88-89	31.49343168762737	35.0	31.5	35.0	19.5	37.0
90-91	31.395375584334666	35.0	31.0	35.0	19.0	36.5
92-93	31.161081649552987	35.0	31.0	35.0	18.5	36.0
94-95	31.06526476326145	34.5	31.0	35.0	18.5	36.0
96-97	30.80436573010801	34.0	30.5	35.0	19.0	36.0
98-99	30.753647916165498	34.5	30.0	35.0	18.5	36.5
100	29.496920678563956	34.0	27.0	35.0	17.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.002886728273971073
1101	2	0.044148320054375745
1101	3	0.03157463438625996
1101	4	-0.021429875226253614
1101	5	-0.034570440149650494
1101	6	-0.05824319037703418
1101	7	0.08708934082056174
1101	8	-0.0026025707251022823
1101	9	-0.14667704457465192
1101	10-11	-0.11740029243703276
1101	12-13	-0.11489008400786815
1101	14-15	-0.04196717271548067
1101	16-17	-0.1162544675846604
1101	18-19	-0.09175412405242866
1101	20-21	-0.030174234883183715
1101	22-23	-0.13826599832545838
1101	24-25	-0.19966632925957128
1101	26-27	-0.22302580141420236
1101	28-29	-0.20949081248926404
1101	30-31	-0.19869797610548545
1101	32-33	-0.2961792139794852
1101	34-35	-0.34923904169636444
1101	36-37	-0.2763601186872151
1101	38-39	-0.3648700362008128
1101	40-41	-0.326954131613995
1101	42-43	-0.372988267808644
1101	44-45	-0.4285352921035681
1101	46-47	-0.4018586482747537
1101	48-49	-0.4329808642057813
1101	50-51	-0.5883090748888193
1101	52-53	-0.4507955814333897
1101	54-55	-0.38126087641036577
1101	56-57	-0.3277991681862247
1101	58-59	-0.304368714044287
1101	60-61	-0.33183865166606097
1101	62-63	-0.28923532350061976
1101	64-65	-0.30006614654986663
1101	66-67	-0.5797429396808838
1101	68-69	-0.4006824243155904
1101	70-71	-0.47389846070923625
1101	72-73	-0.4887592988280147
1101	74-75	-0.5494275789101835
1101	76-77	-0.516560835748443
1101	78-79	-0.6953392111536232
1101	80-81	-0.3549223698624573
1101	82-83	-0.4904010395244711
1101	84-85	-0.8753837180066917
1101	86-87	-1.1094371038355106
1101	88-89	-0.6614164071855946
1101	90-91	-0.793869269131644
1101	92-93	-0.6007148019823134
1101	94-95	-0.7897692107970258
1101	96-97	-0.7849563253629235
1101	98-99	-0.7887284973319098
1101	100	-0.8273112880014999
1102	1	-0.08616250524967484
1102	2	-0.09219585736758518
1102	3	0.029463057535032533
1102	4	-0.14020176942170082
1102	5	-0.11223104868533795
1102	6	-0.09449112592369602
1102	7	0.09180106449583292
1102	8	-0.08902849509640731
1102	9	-0.11300348914709701
1102	10-11	-0.064499169258049
1102	12-13	-0.07696976001602707
1102	14-15	-0.11461743162468707
1102	16-17	-0.1430263418316926
1102	18-19	-0.1593764768813699
1102	20-21	-0.11120971840647798
1102	22-23	-0.20748208188420847
1102	24-25	-0.14280900098241744
1102	26-27	-0.2087351805040285
1102	28-29	-0.5077923710424415
1102	30-31	-0.2698063794037395
1102	32-33	-0.2977923944155947
1102	34-35	-0.26191836618756525
1102	36-37	-0.24751073689880343
1102	38-39	-0.3517829235891412
1102	40-41	-0.7919550777784892
1102	42-43	-0.1854600825890671
1102	44-45	-0.27428060420255207
1102	46-47	-0.3111003526194551
1102	48-49	-0.5697057700903514
1102	50-51	-0.43721078987082507
1102	52-53	-0.5331838631423409
1102	54-55	-0.3175614720830211
1102	56-57	-0.25093973793701707
1102	58-59	-0.30915342491276476
1102	60-61	-0.7386973329261011
1102	62-63	-0.32763279330255557
1102	64-65	-0.3575055991800937
1102	66-67	-0.36684412719350945
1102	68-69	-0.5192680224637911
1102	70-71	-0.234402294914986
1102	72-73	-0.4563543795853988
1102	74-75	-0.32048034829069394
1102	76-77	-0.5644307742879207
1102	78-79	-0.4037074796135087
1102	80-81	-0.3435207137559786
1102	82-83	-0.5088602238170878
1102	84-85	-0.4604230057669447
1102	86-87	-0.5028064071195111
1102	88-89	-0.44357029499809997
1102	90-91	-0.4113974072507851
1102	92-93	-0.40670718630481417
1102	94-95	-0.681897777973365
1102	96-97	-0.5518089925447391
1102	98-99	-0.6249100212745837
1102	100	-0.8798130826663524
1103	1	-0.03992589874133756
1103	2	-0.04852204981420982
1103	3	-0.07437306917693931
1103	4	-0.02725564409226422
1103	5	-0.031589814218207835
1103	6	-0.11622991304333397
1103	7	0.026257475219694015
1103	8	-0.0910729676902804
1103	9	-0.12499976507322685
1103	10-11	-0.13609694600700806
1103	12-13	-0.11231590706707095
1103	14-15	-0.11898925644397451
1103	16-17	-0.16997347584912603
1103	18-19	-0.26584977504823115
1103	20-21	-0.2905725548940197
1103	22-23	-0.35937424923996986
1103	24-25	-0.3164526725734831
1103	26-27	-0.40159239130716884
1103	28-29	-0.45518922733820943
1103	30-31	-0.3614130553630446
1103	32-33	-0.4485298439754217
1103	34-35	-0.5498216185829676
1103	36-37	-0.5412293775952222
1103	38-39	-0.42048580642165234
1103	40-41	-0.3012123622060585
1103	42-43	-0.34616263442563167
1103	44-45	-0.3540873853158715
1103	46-47	-0.41689726092886303
1103	48-49	-0.43325183019955205
1103	50-51	-0.5393319715152884
1103	52-53	-0.37885411008804226
1103	54-55	-0.36927063118614
1103	56-57	-0.32624111372206244
1103	58-59	-0.2036371058611124
1103	60-61	-0.24411341118373997
1103	62-63	-0.17122963321475027
1103	64-65	-0.31903376611361267
1103	66-67	-0.2767352171500619
1103	68-69	-0.23810282805492022
1103	70-71	-0.30285117714332443
1103	72-73	-0.2770671161969389
1103	74-75	-0.2420166589806314
1103	76-77	-0.40133254689869347
1103	78-79	-0.3766832024827096
1103	80-81	-0.3699609825165666
1103	82-83	-0.30431514330445
1103	84-85	-0.3884578272059862
1103	86-87	-0.5934501102032108
1103	88-89	-0.38509883504089615
1103	90-91	-0.37915581567004963
1103	92-93	-0.48291233619176666
1103	94-95	-0.5707609463342145
1103	96-97	-0.5875575989030963
1103	98-99	-0.5248753609005341
1103	100	-0.5417131305702583
1104	1	0.03879646872088571
1104	2	0.042723815770678186
1104	3	0.09933438307552933
1104	4	0.08639067169016812
1104	5	0.053375252912786664
1104	6	0.03565269660821713
1104	7	0.1350168319560865
1104	8	0.04723935648614486
1104	9	0.07967410096289029
1104	10-11	-0.0029218341406078707
1104	12-13	0.07910630013864761
1104	14-15	0.11187233921864248
1104	16-17	-0.015402569456725246
1104	18-19	-0.09101280532524214
1104	20-21	-0.034624851330818274
1104	22-23	-0.06629783924574895
1104	24-25	0.03822182534493379
1104	26-27	-0.0535536981809841
1104	28-29	-0.16464771536654155
1104	30-31	-0.10289608123918725
1104	32-33	-0.06488194477030618
1104	34-35	-0.08429080679449896
1104	36-37	-0.15554384385261244
1104	38-39	-0.2403368717659049
1104	40-41	-0.02848886218774993
1104	42-43	-0.11393551325718221
1104	44-45	-0.11056192073835547
1104	46-47	-0.21368604442350403
1104	48-49	-0.2639116323943753
1104	50-51	-0.2730742720760517
1104	52-53	-0.24091737488630827
1104	54-55	-0.17577719746529397
1104	56-57	-0.7248021597771697
1104	58-59	-0.11765738445058105
1104	60-61	-0.022097991025688657
1104	62-63	-0.11838405353802273
1104	64-65	-0.08519459242236849
1104	66-67	-0.16694469356311004
1104	68-69	-0.2152798716226556
1104	70-71	-0.23739193531658032
1104	72-73	-0.1317118320162436
1104	74-75	-0.1859894497575354
1104	76-77	-0.23365830675108867
1104	78-79	-0.18966942428937728
1104	80-81	-0.2268376366775442
1104	82-83	-0.19163181613196656
1104	84-85	-0.19905059040679518
1104	86-87	-0.2720440382976079
1104	88-89	-0.2449584475223574
1104	90-91	-0.4696901353344707
1104	92-93	-0.5636041878280373
1104	94-95	-0.29426744143369277
1104	96-97	-0.3126987734867832
1104	98-99	-0.31160117732497383
1104	100	-0.4035627355230247
1105	1	-1.6831504982661727E-4
1105	2	-0.055233242397548565
1105	3	0.04719323254147412
1105	4	0.07676967750905561
1105	5	0.0843639615617846
1105	6	0.009395019956677686
1105	7	0.09236496827791285
1105	8	0.02208716228450669
1105	9	-0.027853020091257008
1105	10-11	-0.08892719168890295
1105	12-13	-0.00139814282192674
1105	14-15	-0.08332237954633115
1105	16-17	-0.07149574862387453
1105	18-19	-0.09557634420790606
1105	20-21	-0.05218619062863894
1105	22-23	-0.10609727147058834
1105	24-25	-0.07880833345695493
1105	26-27	-0.1621925216624831
1105	28-29	-0.14990834915714402
1105	30-31	-0.07686313265190137
1105	32-33	-0.08298818756364312
1105	34-35	-0.23983322508826177
1105	36-37	-0.14014225923665435
1105	38-39	-0.023868993347214484
1105	40-41	-0.15669559527150767
1105	42-43	-0.23551021630206748
1105	44-45	-0.23095340556132982
1105	46-47	-0.15913346720513744
1105	48-49	-0.1637760759351039
1105	50-51	-0.29039657457224166
1105	52-53	-0.15543064630994508
1105	54-55	-0.18247045104904203
1105	56-57	-0.1250448504668995
1105	58-59	-0.12255621637786618
1105	60-61	-0.1468725517084053
1105	62-63	-0.1309306685035665
1105	64-65	-0.20093392460319137
1105	66-67	-0.19100694131233809
1105	68-69	-0.18757971804778606
1105	70-71	-0.18047230802432068
1105	72-73	-0.1345480301651989
1105	74-75	-0.18433775287515886
1105	76-77	-0.19528467186878373
1105	78-79	-0.18102187052561902
1105	80-81	-0.12256526525953859
1105	82-83	-0.16510559265124414
1105	84-85	-0.18580521815369977
1105	86-87	-0.14038362786834924
1105	88-89	-0.16151187155021418
1105	90-91	-0.24311892161325588
1105	92-93	-0.2476957726727136
1105	94-95	-0.29660612506332384
1105	96-97	-0.15684797819433172
1105	98-99	-0.30298422138751846
1105	100	-0.18874725129359504
1106	1	0.07450324242864781
1106	2	0.06296578490773896
1106	3	0.06002494740585007
1106	4	0.07702694402493648
1106	5	0.08449650407937526
1106	6	0.0814940718379944
1106	7	0.23666590063899662
1106	8	0.04529559468344502
1106	9	0.07629314955585897
1106	10-11	0.039079990782333596
1106	12-13	0.11160209215914563
1106	14-15	0.0684664072503054
1106	16-17	0.03320134296850341
1106	18-19	0.02182085937190692
1106	20-21	0.02666209609267156
1106	22-23	-0.001243534691724335
1106	24-25	0.012800964602767806
1106	26-27	0.010453051809236058
1106	28-29	0.001058642118138664
1106	30-31	-0.06374324950490262
1106	32-33	-0.005837973313163047
1106	34-35	-0.04381718439423565
1106	36-37	-0.0022944153520256805
1106	38-39	0.01174375925700133
1106	40-41	-0.04634217727903689
1106	42-43	0.06696300473746675
1106	44-45	-0.003012728372603135
1106	46-47	0.012976883411667473
1106	48-49	-0.04953810375194223
1106	50-51	-0.11463448789489661
1106	52-53	-0.1233331336663781
1106	54-55	-0.04827292489946444
1106	56-57	-0.005618599297271487
1106	58-59	0.07858770923011349
1106	60-61	0.09923139679985127
1106	62-63	0.07914155404900924
1106	64-65	0.07749805876439098
1106	66-67	0.10067993206037329
1106	68-69	0.06915324150714497
1106	70-71	0.022153297906491787
1106	72-73	0.0460830970231072
1106	74-75	0.02697098357375438
1106	76-77	0.0658391397561715
1106	78-79	0.11066913372010134
1106	80-81	0.10597007148692228
1106	82-83	0.11747577287069788
1106	84-85	0.0669568111878398
1106	86-87	-0.028528309550193143
1106	88-89	0.06041931618995022
1106	90-91	0.12438510846999762
1106	92-93	0.03305888712046112
1106	94-95	0.06067605090045092
1106	96-97	0.05624664280268021
1106	98-99	0.07478138479362784
1106	100	0.015481408164358612
1107	1	-0.11434351633214845
1107	2	-0.11541655952855479
1107	3	-0.04796655661593263
1107	4	-0.06541980337309639
1107	5	-0.005932118891344373
1107	6	0.009678230470363758
1107	7	0.14031413006248528
1107	8	-0.06255849598368002
1107	9	-0.08750747685059679
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2316	80-81	-1.0502785641444135
2316	82-83	0.02712011004776116
2316	84-85	-1.5504415187801044
2316	86-87	0.06225812567089761
2316	88-89	0.2889340066874375
2316	90-91	-0.6195173079141334
2316	92-93	0.26378865614989166
2316	94-95	0.29143214704367537
2316	96-97	0.3615278842363665
2316	98-99	0.2994414977534312
2316	100	0.28571682793666753
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	17248.0
1	1450.0
2	1010.0
3	811.0
4	696.0
5	782.0
6	813.0
7	1424.0
8	2409.0
9	2827.0
10	4404.0
11	8375.0
12	13961.0
13	17835.0
14	20305.0
15	22137.0
16	24761.0
17	28541.0
18	33549.0
19	39835.0
20	46422.0
21	55115.0
22	63605.0
23	70537.0
24	74199.0
25	76649.0
26	81756.0
27	90693.0
28	104807.0
29	123197.0
30	149143.0
31	187412.0
32	243608.0
33	338534.0
34	526223.0
35	951090.0
36	1183656.0
37	1273714.0
38	1330166.0
39	322478.0
40	386.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.132480331344425	17.53487867483505	12.468785557076261	23.86385543674427
2	16.007028519230996	20.51880723067091	38.54949260035539	24.92467164974271
3	20.59963680006904	24.39385884211777	29.265548265158326	25.74095609265487
4	13.12627345487475	15.522339561332249	37.35504920696222	33.99633777683078
5	13.724076363594229	37.53288795938761	34.097610331404084	14.645425345614079
6	32.0724356681062	35.601519710559714	17.343538381928294	14.982506239405788
7	28.174191248452168	31.9601460783314	21.58135091839416	18.284311754822266
8	26.397733826580815	33.30870024194292	19.986692274078457	20.3068736573978
9	26.48321138421348	16.17839538352337	20.171392605522243	37.16700062674091
10-11	25.724911352644163	24.929133020974522	27.889472599554306	21.45648302682701
12-13	26.747774595247858	22.80672519445672	27.561384968402447	22.884115241892978
14-15	23.45389769558012	24.448108865510942	26.114851165757447	25.983142273151493
16-17	22.972509196980052	27.36771751880815	26.372420969846416	23.287352314365386
18-19	22.779924090390082	27.37003236184357	27.519093700671014	22.33094984709533
20-21	23.87897371159894	25.701368108560597	27.225091480771233	23.194566699069227
22-23	23.33935214691167	25.61106441297335	27.446096037531948	23.603487402583028
24-25	23.801686641051646	25.60987637625733	27.3273172147782	23.261119767912824
26-27	23.443313094129433	25.29113277989109	28.16694298852823	23.098611137451243
28-29	22.883278714058026	26.895214526120238	27.419925657436465	22.801581102385267
30-31	23.551399704746686	25.701419613016245	27.262343742710755	23.48483693952631
32-33	23.481108345197864	25.92402583634788	27.465673830549093	23.129191987905166
34-35	23.990926282634874	25.77254773244912	27.382922266198577	22.853603718717423
36-37	23.716796548724574	25.50925546932248	27.886998949096746	22.8869490328562
38-39	23.327681529530665	26.258270705117216	27.57852177885788	22.835525986494243
40-41	23.449062987136372	26.28291755264152	27.213605591092193	23.054413869129913
42-43	23.031346756438005	26.424760596452273	26.690906525460505	23.852986121649216
44-45	23.361070614142847	25.778236087816296	27.02349900726603	23.83719429077483
46-47	23.854121779477637	26.21313787780524	26.880756444039523	23.0519838986776
48-49	23.59108493229907	26.19319681050901	26.938315774731215	23.2774024824607
50-51	22.701316874471313	26.502282604159515	27.62917801024435	23.167222511124823
52-53	23.39643200232543	26.88598770933809	26.41080861338343	23.30677167495305
54-55	22.87704546451701	26.035887219433235	26.99761830876287	24.089449007286884
56-57	23.536493288590606	25.966516192606797	26.769388516032812	23.72760200276979
58-59	24.20008112444094	25.973521584287234	26.57748792103941	23.248909370232415
60-61	23.52196679009624	26.16161846097485	27.55786892822874	22.758545820700174
62-63	23.05047418370166	26.31400314901918	27.261351947831468	23.37417071944769
64-65	22.85981945142808	26.911700453644883	26.786570812053164	23.441909282873876
66-67	23.260749880867696	26.08078578131056	26.48012778624652	24.17833655157522
68-69	22.800882993978014	26.240605207693978	26.870357524687748	24.08815427364026
70-71	23.519902830426055	26.0222710458227	26.696597367027874	23.76122875672337
72-73	23.220000703149097	25.795896858681417	27.31464563682669	23.669456801342804
74-75	22.659218969125316	27.101472548765827	26.672116753564463	23.567191728544394
76-77	23.008968966583467	26.012652476925226	26.54418032325824	24.43419823323307
78-79	23.018197256187946	26.073997084439217	26.945469746213597	23.96233591315924
80-81	23.45249322385682	26.025343587812344	26.664439690128443	23.857723498202393
82-83	23.219807747766477	25.828196581414552	27.217235382172277	23.734760288646694
84-85	22.970876553098474	26.345090973735562	26.920082800847183	23.763949672318777
86-87	22.82326388306297	26.542576608492713	26.715196897389003	23.918962611055317
88-89	22.525901168395276	26.3299497022291	26.44382403662513	24.700325092750496
90-91	23.071070537350355	25.655249731325817	26.640766793209803	24.63291293811402
92-93	23.414040506497308	26.06459516236263	26.712718291378078	23.808646039761978
94-95	23.130995039974366	25.797473232680424	26.84546790924539	24.226063818099814
96-97	22.22747017263789	25.77915915848327	27.65776041463085	24.335610254247992
98-99	22.73994170237637	26.127424079456713	26.60752254808209	24.52511167008483
100	22.690293742783364	25.00331986871834	26.75748921758586	25.548897170912436
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10729.0
1	8332.5
2	4469.0
3	2453.0
4	1645.0
5	1493.0
6	1986.0
7	3144.0
8	4890.5
9	6091.0
10	5877.5
11	5456.5
12	5710.5
13	6094.5
14	6515.0
15	6927.5
16	7317.0
17	7773.0
18	8313.0
19	8966.0
20	9599.5
21	10592.0
22	12223.0
23	14802.5
24	18086.5
25	21978.5
26	27591.5
27	34603.5
28	42691.0
29	51779.0
30	61884.5
31	71809.5
32	82080.5
33	92992.5
34	104019.0
35	115778.5
36	130411.5
37	145707.5
38	158595.0
39	170460.0
40	182921.5
41	196253.0
42	209318.0
43	222277.0
44	235612.5
45	250101.0
46	268648.5
47	317605.0
48	406056.0
49	410299.5
50	338026.0
51	307300.5
52	297705.5
53	290330.0
54	282711.0
55	268385.0
56	247366.0
57	223162.0
58	200027.0
59	176112.0
60	151282.5
61	127885.5
62	104951.0
63	82531.0
64	62169.5
65	46549.0
66	34855.5
67	26950.0
68	20759.0
69	15557.5
70	11941.5
71	8915.0
72	6687.5
73	5069.0
74	3842.5
75	2879.5
76	2121.0
77	1394.5
78	868.0
79	627.5
80	475.0
81	327.5
82	242.0
83	194.0
84	149.0
85	117.0
86	99.0
87	79.5
88	54.5
89	42.5
90	40.5
91	36.0
92	32.5
93	31.0
94	20.5
95	19.5
96	17.5
97	11.0
98	9.0
99	5.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2017497896587609
2	0.17911347652769571
3	0.36821824484184634
4	0.36439687427810263
5	0.3730613012854799
6	0.30553715267821685
7	0.32413979688088584
8	0.3135912218872184
9	0.32756310801090627
10-11	0.30068746191068796
12-13	0.30946467242428677
14-15	0.2954131744138542
16-17	0.2901587898887066
18-19	0.31245675250110694
20-21	0.3155549817602533
22-23	0.30753408417072875
24-25	0.3121316706302329
26-27	0.3009395662187127
28-29	0.3279412644729435
30-31	0.30986936618190547
32-33	0.3087879713869571
34-35	0.31095076097685376
36-37	0.31815563672724556
38-39	0.3251747514085665
40-41	0.35127418161302437
42-43	0.32034496361272374
44-45	0.30476757110635183
46-47	0.31201888712401127
48-49	0.33364014869908204
50-51	0.3240137447268735
52-53	0.3523157704646004
54-55	0.3524617255902989
56-57	0.35776255038271426
58-59	0.3433859705014076
60-61	0.3262362432318286
62-63	0.34880621312393995
64-65	0.34435458179013434
66-67	0.33113900859052064
68-69	0.34170748655587435
70-71	0.36112615259767616
72-73	0.36471532182508126
74-75	0.35282661340454524
76-77	0.3368312584927639
78-79	0.3472736843041052
80-81	0.35996514591598316
82-83	0.36517309017386307
84-85	0.3579615800995759
86-87	0.3584790573634162
88-89	0.3461922895091569
90-91	0.3582866619704499
92-93	0.34026783827057505
94-95	0.3599253399726109
96-97	0.36902099803318833
98-99	0.36045608588424194
100	0.38128786291576144
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7536563.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.995564005578935
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.18116961929042	41.53816431425683
2	14.405076390226176	16.132407540283275
3	4.987696654948644	8.378666618477663
4	2.235863925846359	5.007938462699792
5	1.1887066086767648	3.3281148495007224
6	0.7206109699081755	2.4210610613169337
7	0.4692787073607547	1.839426812613214
8	0.32869623209870485	1.4724424722300524
9	0.23909774255809374	1.2049571652301054
>10	1.1714336389000375	11.44122562631411
>50	0.05304868719983419	2.0075976035714342
>100	0.01795769804184833	1.647153818829414
>500	6.505879600348526E-4	0.24258856660099629
>1k	4.027382955004009E-4	0.42247741809797795
>5k	1.2391947549538453E-4	0.6182943308226942
>10k+	1.858792132430768E-4	2.2974833391548635
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	57587	0.7641016203274622	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19217	0.2549836045953573	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	16157	0.2143815423555804	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15339	0.20352778846272498	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	12585	0.16698593244692572	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	11555	0.15331922522242566	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	9363	0.12423434926504297	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	9264	0.12292075313375607	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9036	0.11989550143745896	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	7979	0.1058705407226079	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.6537295581553554E-5
2	0.0	0.0	0.0	0.0	2.6537295581553554E-5
3	0.0	0.0	0.0	0.0	2.6537295581553554E-5
4	0.0	0.0	0.0	1.3268647790776777E-5	3.980594337233033E-5
5	0.0	0.0	0.0	1.3268647790776777E-5	3.980594337233033E-5
6	0.0	0.0	0.0	1.3268647790776777E-5	7.961188674466066E-5
7	0.0	0.0	0.0	1.3268647790776777E-5	7.961188674466066E-5
8	0.0	0.0	0.0	1.3268647790776777E-5	1.0614918232621422E-4
9	0.0	0.0	0.0	2.6537295581553554E-5	1.3268647790776777E-4
10-11	0.0	0.0	0.0	2.6537295581553554E-5	1.3268647790776777E-4
12-13	1.3268647790776777E-5	0.0	0.0	3.980594337233033E-5	1.9239539296626325E-4
14-15	1.3268647790776777E-5	6.6343238953883884E-6	0.0	5.9708915058495493E-5	2.5210430802475876E-4
16-17	1.3268647790776777E-5	1.3268647790776777E-5	0.0	7.961188674466066E-5	3.051788991878659E-4
18-19	1.3268647790776777E-5	1.3268647790776777E-5	0.0	8.624621064004905E-5	3.317161947694194E-4
20-21	1.3268647790776777E-5	1.3268647790776777E-5	0.0	1.3932080180315617E-4	3.781564620371382E-4
22-23	1.3268647790776777E-5	1.3268647790776777E-5	0.0	2.454699841293704E-4	4.113280815140801E-4
24-25	1.3268647790776777E-5	1.3268647790776777E-5	0.0	4.179624054094685E-4	4.4449970099102207E-4
26-27	1.3268647790776777E-5	1.3268647790776777E-5	0.0	8.690964302958789E-4	4.77671320467964E-4
28-29	1.3268647790776777E-5	1.3268647790776777E-5	0.0	0.003191109793681815	4.909399682587408E-4
30-31	1.3268647790776777E-5	1.3268647790776777E-5	0.0	0.010827216597273849	5.042086160495175E-4
32-33	1.3268647790776777E-5	1.3268647790776777E-5	0.0	0.028627107608600898	5.705518550034014E-4
34-35	1.3268647790776777E-5	1.3268647790776777E-5	0.0	0.05604013394434572	5.97089150584955E-4
36-37	1.3268647790776777E-5	1.9902971686165164E-5	0.0	0.09689430049214741	6.103577983757318E-4
38-39	1.3268647790776777E-5	2.6537295581553554E-5	0.0	0.15735952847471718	6.435294178526737E-4
40-41	1.3268647790776777E-5	2.6537295581553554E-5	0.0	0.22161958972544912	6.634323895388388E-4
42-43	1.3268647790776777E-5	2.6537295581553554E-5	0.0	0.2894754545274816	6.899696851203924E-4
44-45	1.3268647790776777E-5	2.6537295581553554E-5	0.0	0.37381097988565876	7.364099523881111E-4
46-47	1.3268647790776777E-5	2.6537295581553554E-5	0.0	0.47686856727662197	7.961188674466067E-4
48-49	1.3268647790776777E-5	2.6537295581553554E-5	0.0	0.5834489806560363	8.757307541912673E-4
50-51	1.3268647790776777E-5	2.6537295581553554E-5	0.0	0.699290910193413	9.420739931451512E-4
52-53	1.3268647790776777E-5	2.6537295581553554E-5	0.0	0.8179126214429575	0.0010216858798898119
54-55	1.3268647790776777E-5	2.6537295581553554E-5	0.0	0.9542944177604566	0.0011012977666344725
56-57	1.3268647790776777E-5	2.6537295581553554E-5	0.0	1.1228858565900663	0.001127835062216026
58-59	1.3268647790776777E-5	2.6537295581553554E-5	0.0	1.305316495065456	0.0011543723577975796
60-61	1.3268647790776777E-5	2.6537295581553554E-5	0.0	1.497499589667067	0.0011676410055883565
62-63	1.3268647790776777E-5	2.6537295581553554E-5	0.0	1.6989375661027446	0.0012339842445422402
64-65	1.3268647790776777E-5	2.6537295581553554E-5	0.0	1.9059483746105488	0.001280424511809959
66-67	1.3268647790776777E-5	2.6537295581553554E-5	0.0	2.1515510452178264	0.0013202304551822894
68-69	1.3268647790776777E-5	2.6537295581553554E-5	0.0	2.43191757303694	0.001346767750763843
70-71	1.3268647790776777E-5	2.6537295581553554E-5	0.0	2.7310791404516888	0.0014396482852992803
72-73	1.3268647790776777E-5	2.6537295581553554E-5	0.0	3.030857699988708	0.0016320436782655437
74-75	1.3268647790776777E-5	2.6537295581553554E-5	0.0	3.3448867872530226	0.0016851182694286506
76-77	1.3268647790776777E-5	2.6537295581553554E-5	0.0	3.686826475145235	0.0017050212411148158
78-79	1.3268647790776777E-5	3.317161947694194E-5	0.0	4.088660839165015	0.0017116555650102043
80-81	1.3268647790776777E-5	3.980594337233033E-5	0.0	4.51587812640855	0.0017514615083825346
82-83	1.3268647790776777E-5	3.980594337233033E-5	0.0	4.9372240370046665	0.001910685281871856
84-85	1.3268647790776777E-5	3.980594337233033E-5	0.0	5.371692640265861	0.001910685281871856
86-87	1.3268647790776777E-5	3.980594337233033E-5	0.0	5.822441874366339	0.001950491225244186
88	1.3268647790776777E-5	3.980594337233033E-5	0.0	6.1922523569430785	0.0019902971686165164
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	25560	0.0	28.087135	1
TACATGG	26195	0.0	27.026304	2
ACATGGG	26885	0.0	25.775383	3
CATGGGG	19375	0.0	23.99577	4
ATGGGGG	11355	0.0	21.237982	5
TATACCG	530	0.0	20.400225	5
GAGTACT	18320	0.0	19.31121	12-13
AGAGTAC	29105	0.0	19.192545	10-11
TACCTGG	5505	0.0	19.128054	2
GAGTACA	19910	0.0	18.678177	1
GTACTTT	19685	0.0	17.912285	14-15
AGTACAT	19520	0.0	17.84508	2
AGTACTT	19540	0.0	17.05956	12-13
GTACCTG	6540	0.0	16.89349	1
GTATAGG	1895	0.0	16.126251	1
CATGGGA	9315	0.0	15.392071	4
TATACTG	3075	0.0	14.828923	5
ACTTTTT	24555	0.0	14.7420635	16-17
TACTTTT	22670	0.0	14.67295	14-15
GTATAAG	2375	0.0	14.648642	1
>>END_MODULE