FastQCFastQC Report
Mon 6 Jun 2016
ERR522992_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522992_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7536563
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT591870.7853314567927051No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179480.23814569054886162No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT161830.2147265271981406No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151820.201444610759573No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC129770.17218724238091024No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC122070.1619703835820121No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG96220.12767092904285415No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA95800.12711364583564153No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86330.11454823637777592No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA83010.11014304531123803No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC75440.10009867893362001No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG251550.027.2031081
TACATGG259500.026.0082762
ACATGGG262900.025.0617623
CATGGGG188800.023.0495854
TACCTGG59300.021.637272
ATGGGGG110400.020.1343485
GAGTACT180950.019.30444312-13
GTACCTG73250.018.3627741
AGAGTAC295100.018.15399410-11
GTACTTT195850.017.73647114-15
GAGTACA195800.017.1980881
AGTACTT192050.017.12381212-13
AGTACAT189700.016.4263362
TATACTG30950.015.94315055
TGGGGGG95450.015.5581046
GTATAGG18500.015.5073641
GTATATA32350.015.4103121
GGACCGT9800.015.345096
ACCTGGG81500.015.2806713
ACTTTTT240700.014.39959116-17
Produced by FastQC (version 0.11.5)