FastQCFastQC Report
Mon 6 Jun 2016
ERR522991_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522991_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7386093
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT707830.9583280362161699No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239150.32378417114433844No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT207590.2810552209402183No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184390.24964483929460407No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC155760.21088280366900336No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC145170.1965450475643889No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG115750.1567134342879246No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA113350.15346408446251625No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111740.15128431228797146No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA102940.13937002959480743No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC91460.12382730626327072No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG260800.026.3726271
TACATGG268850.025.4068932
TACCTGG55300.024.6529482
ACATGGG275050.023.8785133
CATGGGG189450.022.0103994
GTACCTG67150.021.2835581
GAGTACT205700.019.65635912-13
ATGGGGG114200.019.5951525
TATCACG6500.019.5274872
AGAGTAC324600.018.98351910-11
ACCTGGG70700.018.75223
AGTACTT217550.018.12129812-13
GTACTTT225500.017.88821214-15
TATAACG7150.017.7522622
GAGTACA205600.017.7212851
GTATAGG19300.017.0512961
ATAACGC7550.016.8127733
AGTACAT200700.016.607142
CATGGGA96350.015.7109724
TAACGCA7350.015.3505114
Produced by FastQC (version 0.11.5)