FastQCFastQC Report
Mon 6 Jun 2016
ERR522991_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522991_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7386093
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT723480.9795165048693538No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218870.2963271651196377No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT208630.2824632725312286No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177990.2409799064268484No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC165150.2235958848609136No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC150430.2036665392650756No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA120580.16325275081155896No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG119300.16151976423800782No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA106450.14412220371446716No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104740.1418070419638637No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG254350.026.4582611
TACCTGG58900.026.1747532
TACATGG261400.025.2276082
ACATGGG264000.023.8892233
CATGGGG182000.023.1619174
GTACCTG73100.021.8091
ATGGGGG105650.020.4170825
GAGTACT208350.019.66504712-13
AGAGTAC328100.019.1577610-11
ACCTGGG76250.018.9213433
GTACTTT227450.017.9170914-15
AGTACTT220750.017.91085412-13
TATCACG6700.016.8368362
GAGTACA204000.016.2291561
AGTACAT199300.015.494662
GTATAAT32200.015.335111
TGGGGGG100600.015.2289556
TACTTTT261750.015.03946614-15
ACTTTTT274750.014.8126316-17
TATAACG6050.014.761222
Produced by FastQC (version 0.11.5)