FastQCFastQC Report
Mon 6 Jun 2016
ERR522990_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522990_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5297471
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155740.29398933944140515No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120210.2269195999374041No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73410.13857555803514543No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87950.042.587211
GTATCAA149750.038.4292221
TACCTGG55950.037.389232
TCAACGC179500.031.4463714
ATCAACG181250.031.0935383
CAACGCA183450.030.8190575
TATAACG5200.030.7370452
GTACCTG71100.030.2446521
TATCAAC188250.030.2409692
ACCTGGG68000.030.2065753
AACGCAG191750.029.6957876
GTACATG169000.028.3441241
TACATGG172750.027.4574152
ACATGGG176750.025.9814953
ACGCAGA217850.025.9652467
CGCAGAG225250.025.152758
CATGGGG114950.024.1640934
ATAACGC6150.023.6927553
TAACGCA6150.022.926524
GCAGAGT250100.022.4650889
Produced by FastQC (version 0.11.5)