FastQCFastQC Report
Mon 6 Jun 2016
ERR522990_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522990_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5297471
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139200.26276689386312824No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114290.215744456175409No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69020.1302885848738011No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88050.043.437651
GTATCAA147450.038.413761
TACCTGG59400.038.399092
GTACCTG74200.031.8276791
ACCTGGG70950.031.1985783
TCAACGC177950.031.110094
CAACGCA180600.030.5495175
ATCAACG181250.030.5186083
AACGCAG188450.029.426036
TATCAAC190100.029.1178952
GTACATG170050.026.8902471
ACGCAGA215100.025.779787
TACATGG175200.025.178762
CGCAGAG223300.024.8195488
ACATGGG178750.023.9781173
CATGGGG119350.022.5624474
TATAACG4600.022.4921252
ATAACGC5650.022.4585443
GCAGAGT253250.021.8284329
CCTGGGG67650.021.813024
Produced by FastQC (version 0.11.5)