##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR522990_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5297471
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.695118482007736	34.0	31.0	34.0	30.0	35.0
2	32.74911934392845	34.0	31.0	34.0	31.0	34.0
3	33.02917656368482	34.0	31.0	34.0	31.0	36.0
4	35.81148023273747	37.0	35.0	37.0	33.0	37.0
5	35.86405569752057	37.0	35.0	37.0	35.0	37.0
6	35.93831245135651	37.0	35.0	37.0	35.0	37.0
7	35.766483667395256	37.0	35.0	37.0	35.0	37.0
8	35.818417505258644	37.0	36.0	37.0	35.0	37.0
9	37.045839231588054	39.0	36.0	39.0	34.0	39.0
10-11	37.16505668459535	39.0	36.5	39.0	34.0	39.0
12-13	37.033829161122355	38.5	36.5	39.0	34.0	39.0
14-15	38.13965012739098	39.0	36.5	41.0	34.5	41.0
16-17	38.16003617575254	39.0	37.0	41.0	34.5	41.0
18-19	38.1639842860867	39.0	37.0	41.0	34.0	41.0
20-21	38.051329304115114	39.0	36.5	41.0	34.0	41.0
22-23	37.96294495996297	39.0	36.5	41.0	34.5	41.0
24-25	37.83575785502177	39.0	36.0	41.0	34.0	41.0
26-27	37.70881728281287	39.0	36.5	41.0	34.0	41.0
28-29	37.66845226712897	39.0	36.5	41.0	34.5	41.0
30-31	37.51508531146277	38.5	36.0	41.0	34.0	41.0
32-33	37.37262016158276	38.0	36.5	41.0	33.0	41.0
34-35	37.195114517852005	38.0	36.0	41.0	33.0	41.0
36-37	37.21209176982752	38.0	36.0	41.0	33.0	41.0
38-39	37.35050989424954	38.0	36.5	41.0	33.0	41.0
40-41	37.24893557699514	38.0	36.5	41.0	33.0	41.0
42-43	37.277202555710076	38.0	36.5	41.0	33.0	41.0
44-45	37.11798950857872	38.0	36.0	41.0	33.0	41.0
46-47	36.86310146860643	38.0	36.0	41.0	32.0	41.0
48-49	36.83186420463652	38.0	36.5	41.0	32.0	41.0
50-51	36.44412465872867	38.0	35.0	40.5	31.0	41.0
52-53	36.50397840781007	38.0	35.5	40.0	31.0	41.0
54-55	36.311035964142135	37.5	35.5	40.0	31.0	41.0
56-57	36.01796385482808	38.0	35.0	40.0	29.5	41.0
58-59	35.88812388024399	36.5	35.0	40.0	30.0	41.0
60-61	35.6234068105328	36.5	35.0	40.0	29.0	41.0
62-63	35.44394362895049	37.5	35.0	39.5	28.5	41.0
64-65	35.303559943980815	37.0	35.0	39.0	28.5	41.0
66-67	35.08409021965387	36.5	34.5	39.0	28.5	41.0
68-69	34.61441299065157	36.0	34.0	38.5	27.0	40.0
70-71	34.37168178929153	36.0	34.0	38.0	26.5	40.0
72-73	34.10424417613612	36.0	34.0	37.0	26.0	39.5
74-75	33.82926324655671	35.5	33.5	37.5	26.0	39.0
76-77	32.336392875015264	34.5	31.0	36.5	24.5	37.5
78-79	33.335675079674814	35.0	33.0	37.0	26.0	38.0
80-81	33.32490201456506	35.0	33.0	36.5	26.0	37.0
82-83	33.14240568754411	35.0	33.0	36.0	26.0	37.5
84-85	32.939167010069525	35.0	33.0	36.0	25.5	37.0
86-87	32.69121567631045	35.0	33.0	36.0	25.0	37.0
88-89	32.52577097637722	35.0	33.0	36.0	25.0	36.5
90-91	32.38301540489792	35.0	32.5	35.5	25.0	37.0
92-93	32.23212727356129	35.0	32.5	35.5	24.0	36.5
94-95	32.11168810551299	35.0	32.0	35.0	24.5	36.0
96-97	31.940088204352605	35.0	32.0	35.0	23.5	36.5
98-99	31.81294951874206	35.0	32.0	35.0	23.0	36.5
100	30.61333492906332	34.0	29.0	35.0	19.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.09813951927670672
1101	2	-0.10145745641956694
1101	3	-0.1092755121551221
1101	4	-0.1270335534381175
1101	5	-0.1304855970128429
1101	6	-0.19659193298699762
1101	7	-0.19027118895419193
1101	8	-0.16539896110533903
1101	9	-0.2152838124656924
1101	10-11	-0.1465414042451485
1101	12-13	-0.20471065552099077
1101	14-15	-0.3642389920408604
1101	16-17	-0.15185699742624337
1101	18-19	-0.13483861109660467
1101	20-21	-0.18503706771208073
1101	22-23	-0.15248645413571893
1101	24-25	-0.25339660794936236
1101	26-27	-0.2612688317616332
1101	28-29	-0.3023536530783275
1101	30-31	-0.3368485082021664
1101	32-33	-0.2506236101556141
1101	34-35	-0.2402807695821565
1101	36-37	-0.3282653511876461
1101	38-39	-0.2438818009817254
1101	40-41	-0.2571894397981822
1101	42-43	-0.3044715447224391
1101	44-45	-0.39215805347892996
1101	46-47	-0.398180407455591
1101	48-49	-0.5265615704662991
1101	50-51	-0.40139607242024056
1101	52-53	-0.580168386912888
1101	54-55	-0.41473552195393637
1101	56-57	-0.4716727643233085
1101	58-59	-0.5489847013464555
1101	60-61	-0.6564728738366341
1101	62-63	-0.4607841438182092
1101	64-65	-0.41795984013720044
1101	66-67	-0.4980639243390428
1101	68-69	-0.4048778186471935
1101	70-71	-0.460721991429871
1101	72-73	-0.5645379626858258
1101	74-75	-0.507817060183136
1101	76-77	-0.5853940399880493
1101	78-79	-0.48094442269113813
1101	80-81	-0.2772680481320364
1101	82-83	-0.37842421162227424
1101	84-85	-0.40197301759926773
1101	86-87	-0.45158660222992353
1101	88-89	-0.46674836474588943
1101	90-91	-0.43937188856914133
1101	92-93	-0.7439951783950676
1101	94-95	-0.6152313810754606
1101	96-97	-0.5196657331704984
1101	98-99	-0.5477068283555617
1101	100	-0.6910176544773599
1102	1	-0.013149249730510348
1102	2	-0.06852047304440134
1102	3	-0.03965284797187252
1102	4	-0.09005004537554129
1102	5	-0.12257673427897231
1102	6	-0.060748077392212
1102	7	-0.12434011713108362
1102	8	-0.03743678258478411
1102	9	-0.09161738582511703
1102	10-11	-0.13516676857950216
1102	12-13	-0.1469590310734148
1102	14-15	-0.20925348947730527
1102	16-17	-0.07367880491949563
1102	18-19	-0.15852948964334246
1102	20-21	-0.18731574616248992
1102	22-23	-0.13153115157470552
1102	24-25	-0.19235048781950326
1102	26-27	-0.2321948698713001
1102	28-29	-0.1827254549427053
1102	30-31	-0.07970305562074032
1102	32-33	-0.14550260644283242
1102	34-35	-0.21929304166732067
1102	36-37	-0.18032030798580223
1102	38-39	-0.1836392525007895
1102	40-41	-0.29272005280633095
1102	42-43	-0.2584808884640353
1102	44-45	-0.349569743274607
1102	46-47	-0.2721365645044145
1102	48-49	-0.19993779208866158
1102	50-51	-0.08411985150031143
1102	52-53	-0.17968572754787004
1102	54-55	-0.20419255484801369
1102	56-57	-0.08993661064231873
1102	58-59	-0.3059556872993312
1102	60-61	-0.3403636049505323
1102	62-63	-0.23301234085272426
1102	64-65	-0.29367161746994697
1102	66-67	-0.3660349268083394
1102	68-69	-0.2743493859972972
1102	70-71	-0.2542887670606646
1102	72-73	-0.36936279078683043
1102	74-75	-0.742368571787452
1102	76-77	-0.26874696205727844
1102	78-79	-0.2962558911856732
1102	80-81	-0.17470293306664786
1102	82-83	-0.1955745469911747
1102	84-85	-0.2430080052774457
1102	86-87	-0.3747451202545662
1102	88-89	-0.24305761908994583
1102	90-91	-0.29168298288117
1102	92-93	-0.3343365425722311
1102	94-95	-0.40557957963632774
1102	96-97	-0.4075892062979882
1102	98-99	-0.49007761531396454
1102	100	-0.2987189417904865
1103	1	-0.09807804662506925
1103	2	-0.06717292326593594
1103	3	-0.11891929174228721
1103	4	-0.014003362387953189
1103	5	-0.08961282801143966
1103	6	-0.06851579572548161
1103	7	-0.06880681774202912
1103	8	-0.050843461503454535
1103	9	-0.08370057219001836
1103	10-11	-0.06934851905749895
1103	12-13	-0.09824579513799137
1103	14-15	-0.16856369235157587
1103	16-17	-0.13216898363371854
1103	18-19	-0.15896215980248485
1103	20-21	-0.16463906685271468
1103	22-23	-0.06148911661479417
1103	24-25	-0.08062968750089539
1103	26-27	-0.16348474070004926
1103	28-29	-0.1506596826152773
1103	30-31	-0.06920470824667291
1103	32-33	0.010284228405296858
1103	34-35	-0.004680637121829534
1103	36-37	-0.12220536468883836
1103	38-39	-0.1611891539822139
1103	40-41	-0.20703692916427485
1103	42-43	-0.24691948424335664
1103	44-45	-0.2673158482975637
1103	46-47	-0.30458831372097706
1103	48-49	-0.2533803603764184
1103	50-51	-0.1208589352729561
1103	52-53	-0.1214892959566356
1103	54-55	-0.11641269589730996
1103	56-57	-0.13992859585177087
1103	58-59	-0.10693951431499471
1103	60-61	-0.08652911065075841
1103	62-63	-0.0671130069892385
1103	64-65	-0.2472299365930155
1103	66-67	-0.17124925956738934
1103	68-69	-0.08908646506242235
1103	70-71	-0.29807752472405724
1103	72-73	-0.4504495743513175
1103	74-75	-0.25357198203032993
1103	76-77	-0.1358372589093264
1103	78-79	-0.17095146226566982
1103	80-81	-0.21372210999080465
1103	82-83	-0.2884642250977265
1103	84-85	-0.13641110737779627
1103	86-87	-0.21052507321610392
1103	88-89	-0.22520444821662977
1103	90-91	-0.2890619902993947
1103	92-93	-0.24751851357172328
1103	94-95	0.04680859842826379
1103	96-97	-0.7605904928876157
1103	98-99	-1.0796030644314278
1103	100	-0.35212216140437747
1104	1	-0.028844853167150575
1104	2	-0.06942488996255491
1104	3	-0.047600023671996894
1104	4	-0.16446702849188455
1104	5	-0.16945252600467597
1104	6	-0.10442369126609918
1104	7	-0.09876926259784824
1104	8	-0.04515850367646124
1104	9	-0.019823103501231287
1104	10-11	-0.1381204332725261
1104	12-13	-0.09065777867612468
1104	14-15	-0.15786394996482755
1104	16-17	-0.03361138507732875
1104	18-19	-0.12006719329053084
1104	20-21	-0.19401503583792135
1104	22-23	-0.1638478700065491
1104	24-25	-0.17695177926623984
1104	26-27	-0.06563904179568425
1104	28-29	-0.11236282923200491
1104	30-31	-0.18026687259215635
1104	32-33	-0.16237532329537885
1104	34-35	-0.1467664559241939
1104	36-37	-0.18249960629397322
1104	38-39	-0.21230764475355812
1104	40-41	-0.17551070591371598
1104	42-43	-0.14625992778993435
1104	44-45	-0.25719728735320757
1104	46-47	-0.20285730827725246
1104	48-49	-0.2787885835075983
1104	50-51	-0.21778857578162558
1104	52-53	-0.22466592493163517
1104	54-55	-0.2863261342674335
1104	56-57	-0.27063048536505363
1104	58-59	-0.22134340642915618
1104	60-61	-0.21849311846373354
1104	62-63	-0.08683765268889232
1104	64-65	-0.3479003440032855
1104	66-67	-0.3562243851903659
1104	68-69	-0.20769920045829338
1104	70-71	-0.3031441527443164
1104	72-73	-0.24536809746934551
1104	74-75	-0.22112868692917687
1104	76-77	-0.29110527789294594
1104	78-79	-0.2625484603143846
1104	80-81	-0.11855825739718995
1104	82-83	-0.12716962661668418
1104	84-85	-0.1370642790653065
1104	86-87	-0.21755121629888663
1104	88-89	-0.25065995426121646
1104	90-91	-0.22671017313280828
1104	92-93	-0.33974745317497934
1104	94-95	-0.4037304512473803
1104	96-97	-0.1481262660984939
1104	98-99	-1.0326109426804102
1104	100	-0.3160736053120239
1105	1	-0.05069001977429366
1105	2	-0.9929884919628442
1105	3	-0.061903052847902984
1105	4	-0.053845864797594345
1105	5	-0.06311085095696711
1105	6	-0.04390904258256256
1105	7	-0.08648879815694954
1105	8	-0.07531390429698348
1105	9	-0.11740493233970284
1105	10-11	-0.01817789849133078
1105	12-13	0.020446996003435913
1105	14-15	-0.04898566010974292
1105	16-17	-0.6233882760129887
1105	18-19	-0.07835351191614137
1105	20-21	-0.13301735734500397
1105	22-23	-0.07457086298099824
1105	24-25	-0.09622750189306828
1105	26-27	-0.046025118390602415
1105	28-29	-0.16967207495577696
1105	30-31	-0.10228138658818153
1105	32-33	-0.060077470071931316
1105	34-35	0.060635748882766904
1105	36-37	-0.11151507399962668
1105	38-39	-0.09032529157820335
1105	40-41	-0.11572067459124469
1105	42-43	-0.11919696406605595
1105	44-45	-0.20921202800782623
1105	46-47	-0.1088934809550679
1105	48-49	-0.09897610571941584
1105	50-51	-0.04255841560083695
1105	52-53	-2.2974545405674007E-4
1105	54-55	-0.15912431309467223
1105	56-57	-0.06618828199940907
1105	58-59	-0.10941060140407188
1105	60-61	-0.10149344043517772
1105	62-63	0.04931249729806808
1105	64-65	0.11629337325290123
1105	66-67	-0.005239553590435264
1105	68-69	0.01820415754691851
1105	70-71	-0.02675604319614422
1105	72-73	-0.21894021369637784
1105	74-75	-0.059826261595027574
1105	76-77	-3.6314098476708523E-4
1105	78-79	-0.023465050241618712
1105	80-81	0.042307922786513075
1105	82-83	0.02603509346140953
1105	84-85	0.05989800901253517
1105	86-87	-0.11429035463007153
1105	88-89	0.03630261423399617
1105	90-91	-0.03437804070259176
1105	92-93	0.004449171413575925
1105	94-95	0.02083702312784652
1105	96-97	0.025452192210416058
1105	98-99	-0.03583247327103933
1105	100	0.02222372078489343
1106	1	-0.06903682974773773
1106	2	-0.1121894507720782
1106	3	-0.04505653055946368
1106	4	-0.07504210718938253
1106	5	-0.0769389199690309
1106	6	-0.021974854105152986
1106	7	-0.05201997624252641
1106	8	0.034359222762937236
1106	9	-0.048558826304272884
1106	10-11	-0.06384824824944957
1106	12-13	0.006629559066020363
1106	14-15	-4.6388727596990975E-6
1106	16-17	0.038124880851697185
1106	18-19	-0.013802509162218257
1106	20-21	-0.03013761623164868
1106	22-23	0.07207208129388931
1106	24-25	-0.006296353315143222
1106	26-27	0.01329546800764092
1106	28-29	-0.09596742029903993
1106	30-31	-0.03985667132570114
1106	32-33	0.05528616545093712
1106	34-35	0.07553084853771708
1106	36-37	-0.04003572713234149
1106	38-39	-0.08545856863921841
1106	40-41	-0.10223319608023473
1106	42-43	-0.041540309002677134
1106	44-45	-0.06574443293286691
1106	46-47	-0.03686627274780818
1106	48-49	0.0032275155506482633
1106	50-51	0.10173731613823378
1106	52-53	-0.01017867289983343
1106	54-55	-0.012447870502377612
1106	56-57	0.010853952023772706
1106	58-59	-0.1431913788024417
1106	60-61	-0.1323488316216057
1106	62-63	-0.019936881144296592
1106	64-65	-0.041407547652255516
1106	66-67	0.03054779122587803
1106	68-69	-0.010645066744920939
1106	70-71	0.024719528556410353
1106	72-73	0.020886056019108423
1106	74-75	-0.004355269062450873
1106	76-77	-0.08599416153357708
1106	78-79	-0.045486971540519505
1106	80-81	0.088904901544133
1106	82-83	0.0956914946658074
1106	84-85	0.2328797881853646
1106	86-87	0.13524776911314973
1106	88-89	0.14968617149750685
1106	90-91	0.11407299345965782
1106	92-93	0.03700247215208208
1106	94-95	-0.008854884108323802
1106	96-97	-0.015167212068615044
1106	98-99	0.011529232020524915
1106	100	0.02646602780527374
1107	1	-0.07284799886002702
1107	2	-0.08792171842995344
1107	3	-0.10456289068730484
1107	4	-0.027275900983006807
1107	5	-0.04902675910594212
1107	6	-0.10904314387474301
1107	7	-0.10391643116417981
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2316	76-77	-0.03576502728270725
2316	78-79	-0.09477482548422245
2316	80-81	-0.11694568580182363
2316	82-83	-0.03133610013767196
2316	84-85	0.041580908893671165
2316	86-87	-0.20060902976521788
2316	88-89	-0.11338385878766388
2316	90-91	-0.05620881825649349
2316	92-93	-0.1663308159508574
2316	94-95	-0.0471820955565434
2316	96-97	0.0768637227879978
2316	98-99	0.16535106858475146
2316	100	-0.11090274832564972
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	21.0
13	101.0
14	421.0
15	1123.0
16	2592.0
17	5313.0
18	8743.0
19	12837.0
20	17884.0
21	24340.0
22	31042.0
23	35519.0
24	38482.0
25	41229.0
26	46607.0
27	55285.0
28	66445.0
29	81995.0
30	102273.0
31	128498.0
32	164163.0
33	220261.0
34	335076.0
35	641209.0
36	1147628.0
37	873431.0
38	992136.0
39	222704.0
40	111.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.40181181942532	18.74258387444457	12.99099377404222	23.86461053208789
2	16.207402520781226	20.78976695138191	39.63895137284284	23.36387915499402
3	18.738740745537026	26.277228683469282	30.051673406252117	24.93235716474157
4	12.935816531287578	16.981011690486213	37.85471177750889	32.22846000071733
5	13.341604082316858	37.271642002920686	34.63075359460443	14.756000320158025
6	30.476125215175948	36.83395033307792	18.65115568762012	14.038768764126008
7	27.20120600943356	32.3060003537537	22.618113435637497	17.874680201175238
8	25.869516588626045	34.80609084928518	20.870984274524275	18.453408287564503
9	26.804110867242127	15.750251393542314	20.39675158202848	37.048886157187084
10-11	24.70199448076053	25.779358029277756	29.01865966558747	20.499987824374244
12-13	25.33431543792533	23.74380057487852	28.71033458065902	22.211549406537124
14-15	22.787893765359392	25.251522558445682	26.368745068994897	25.591838607200028
16-17	22.43710569241361	27.8914788503609	26.970785206909255	22.70063025031624
18-19	22.272208456187055	27.072897314816153	28.682565422645595	21.972328806351197
20-21	23.099275586859026	26.410211081328498	28.54574375778734	21.944769574025138
22-23	22.860645677798022	26.27502341494972	28.383279720185335	22.481051187066925
24-25	22.5539721568787	26.385490097510484	28.496239827969426	22.564297917641394
26-27	22.464365586284472	26.496408453582497	28.540247039342248	22.498978920790776
28-29	22.448506280737156	26.640232983410794	28.541345673079597	22.369915062772446
30-31	22.972810207230538	26.402896685281778	28.462704299133595	22.16158880835409
32-33	22.645074290262418	26.570386254994105	28.380384871190127	22.40415458355335
34-35	22.865058316338597	26.644298368071308	28.306110609905673	22.184532705684425
36-37	22.8457639425919	26.57177055373748	28.216316849454337	22.366148654216282
38-39	22.676660909349103	26.74165306150765	28.16311806175825	22.418567967384995
40-41	22.77740483977048	26.511276739214644	28.29958265342414	22.41173576759074
42-43	22.62910672273714	26.602419195447712	28.091966577446474	22.67650750436867
44-45	22.527240131967993	26.80046518972735	28.08238928783704	22.589905390467614
46-47	22.54376600157631	26.552499236059106	27.899366917908896	23.004367844455686
48-49	22.623899417616947	26.674308800090024	27.86000376851642	22.841788013776608
50-51	22.556930460814886	26.92733060475169	27.76400103534815	22.751737899085274
52-53	22.606073946736345	26.975025688666765	27.57369480523526	22.845205559361634
54-55	22.484447825970822	26.782340164123585	27.767330849667022	22.965881160238574
56-57	22.56838003504776	26.9436017263749	27.456981735938186	23.031036502639154
58-59	22.48284133001543	26.838891424761417	27.69365351020761	22.98461373501554
60-61	22.648135454742295	26.904862989558637	27.67402884905264	22.772972706646428
62-63	22.495568505524503	26.933537965495695	27.63629087215964	22.93460265682016
64-65	22.39643810392897	26.668414100716763	27.641788982330777	23.293358813023488
66-67	22.330387395511455	26.77654359697894	27.462594039926746	23.430474967582853
68-69	22.19134163151147	26.86888587193782	27.52594270098213	23.41382979556858
70-71	22.279332509037367	26.56703697061794	27.56908512586244	23.58454539448225
72-73	22.180820879264846	26.876161699071847	27.28492497354262	23.658092448120684
74-75	22.189223917697195	26.858169893243883	27.251217110731048	23.70138907832787
76-77	22.142456520167315	26.71615716567012	27.37861260600354	23.76277370815903
78-79	22.02393835345878	26.725450832194138	27.349540981926125	23.901069832420955
80-81	22.03971209940776	26.867720500047803	27.12878933245945	23.963778068084977
82-83	22.133924414244802	26.803193671779635	27.203030309036663	23.8598516049389
84-85	22.116078619550912	26.842705305236848	27.055006144010623	23.986209931201614
86-87	21.89808317939041	26.837631087446752	27.218769596811125	24.04551613635171
88-89	21.78360783359505	26.653200348962685	27.28770799415451	24.275483823287757
90-91	22.07977877549541	26.581042030994688	27.17348092204815	24.165698271461753
92-93	21.939497602577234	26.587310389064793	27.155044790809633	24.318147217548336
94-95	21.75732798103982	26.4187785840931	27.227078099847706	24.596815335019375
96-97	21.716459905624312	26.346888503831828	27.33713096330063	24.599520627243226
98-99	21.68941918959286	26.35182380116014	27.21149954467267	24.747257464574325
100	21.6657894697099	26.321251172322764	27.27842155895896	24.734537799008375
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	984.0
1	728.0
2	1108.5
3	1584.5
4	1098.0
5	877.5
6	1207.0
7	2016.0
8	3199.5
9	3958.0
10	3711.5
11	3332.0
12	3501.5
13	3837.0
14	4186.0
15	4569.5
16	5070.0
17	5534.5
18	6050.0
19	6620.5
20	7326.0
21	8401.0
22	9746.0
23	11690.5
24	14286.5
25	18016.5
26	23231.0
27	29340.0
28	36214.5
29	44126.0
30	53873.5
31	64068.5
32	74546.0
33	85454.0
34	96396.0
35	107240.5
36	121061.0
37	133988.5
38	142629.5
39	150962.5
40	157081.0
41	162276.0
42	167992.5
43	175986.0
44	184179.0
45	191041.0
46	198080.5
47	204139.5
48	210847.0
49	216004.0
50	215452.0
51	210973.5
52	203600.5
53	196487.5
54	189320.0
55	175942.0
56	157954.5
57	140309.5
58	125064.0
59	108418.5
60	89961.5
61	73456.5
62	60088.0
63	47219.0
64	35033.5
65	26123.5
66	19112.0
67	14527.0
68	11254.0
69	8178.0
70	6310.5
71	5006.0
72	3911.0
73	3200.0
74	2522.5
75	1843.0
76	1242.5
77	743.0
78	449.5
79	288.0
80	190.5
81	133.0
82	86.5
83	52.5
84	34.0
85	19.0
86	21.0
87	15.0
88	8.5
89	5.0
90	3.5
91	3.5
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.029806675675997093
2	0.07762194450899305
3	0.003058062988924338
4	1.8876932030397146E-5
5	0.0016422930866445517
6	0.001170369785884623
7	0.0
8	3.0203091248635433E-4
9	0.0
10-11	3.775386406079429E-5
12-13	7.550772812158858E-5
14-15	0.011892467179150202
16-17	9.816004655806515E-4
18-19	2.3596165037996432E-4
20-21	1.9820778631917004E-4
22-23	0.002029270193267693
24-25	7.645157472310845E-4
26-27	0.007163795705535717
28-29	0.00833416549142034
30-31	0.009816004655806516
32-33	0.009023173510529836
34-35	0.0045682175513561095
36-37	0.0038603326002162163
38-39	0.002916485998696359
40-41	0.0036810017459274432
42-43	0.0016989238827357432
44-45	0.005238348638435208
46-47	0.017413969798041367
48-49	0.018065223953090067
50-51	0.013251606285338797
52-53	0.007324249627794093
54-55	0.0011420543878390274
56-57	0.0024162472998908347
58-59	0.002812662872529175
60-61	0.0032845861732891035
62-63	0.0030391860568939407
64-65	0.0033978477654714864
66-67	0.0022652318436476573
68-69	0.00688064172507976
70-71	3.9641557263834007E-4
72-73	0.005153402444298421
74-75	0.007503580482082865
76-77	0.006795695530942973
78-79	0.004521025221280116
80-81	3.3034631053195006E-4
82-83	0.001085423591747836
84-85	6.701310870790987E-4
86-87	0.0010004773976110487
88-89	0.0010665466597174387
90-91	8.872158054286658E-4
92-93	2.1708471834956715E-4
94-95	0.0010099158636262472
96-97	0.0024917550280124236
98-99	0.003454478561562678
100	0.005568694948967158
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5297471.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.492459924075405
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.64365723574625	49.93279255323341
2	13.262447465274798	16.841308283682316
3	3.761436827124949	7.164686310095167
4	1.5707678379879724	3.9892765601391162
5	0.7882788450884843	2.502488149038852
6	0.46862226750017455	1.7852388323270512
7	0.31951627516442427	1.4200812007176942
8	0.21375172172110307	1.0857298100063406
9	0.15005343951820307	0.8574535795571264
>10	0.7529071501599955	8.48239618310939
>50	0.044791174288329086	1.9490116485277753
>100	0.022337221946960466	2.471898637606529
>500	0.0010555547896140575	0.43778267627797085
>1k	2.638885822301727E-4	0.3079078383145692
>5k	3.769836889002106E-5	0.16520366138425352
>10k+	7.539673778004212E-5	0.6067440759822432
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13920	0.26276689386312824	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11429	0.215744456175409	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6902	0.1302885848738011	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.775386406079429E-5
2	0.0	0.0	0.0	0.0	3.775386406079429E-5
3	0.0	0.0	0.0	0.0	3.775386406079429E-5
4	0.0	0.0	0.0	0.0	3.775386406079429E-5
5	0.0	0.0	0.0	0.0	3.775386406079429E-5
6	0.0	0.0	0.0	0.0	7.550772812158858E-5
7	0.0	0.0	0.0	0.0	7.550772812158858E-5
8	0.0	0.0	0.0	0.0	7.550772812158858E-5
9	0.0	0.0	0.0	5.663079609119144E-5	9.438466015198573E-5
10-11	1.8876932030397146E-5	0.0	0.0	6.606926210639001E-5	9.438466015198573E-5
12-13	1.8876932030397146E-5	0.0	0.0	7.550772812158858E-5	1.038231261671843E-4
14-15	1.8876932030397146E-5	0.0	0.0	7.550772812158858E-5	1.4157699022797858E-4
16-17	1.8876932030397146E-5	0.0	0.0	9.438466015198573E-5	2.831539804559572E-4
18-19	1.8876932030397146E-5	0.0	0.0	1.038231261671843E-4	3.209078445167515E-4
20-21	1.8876932030397146E-5	0.0	0.0	1.5101545624317717E-4	3.397847765471486E-4
22-23	1.8876932030397146E-5	0.0	0.0	4.7192330075992864E-4	3.775386406079429E-4
24-25	1.8876932030397146E-5	0.0	0.0	0.0015101545624317718	4.152925046687372E-4
26-27	1.8876932030397146E-5	0.0	0.0	0.00284097827057477	4.530463687295315E-4
28-29	1.8876932030397146E-5	0.0	0.0	0.007163795705535717	4.530463687295315E-4
30-31	1.8876932030397146E-5	0.0	0.0	0.020443717388920108	4.7192330075992864E-4
32-33	1.8876932030397146E-5	0.0	0.0	0.054620402829954146	5.09677164820723E-4
34-35	1.8876932030397146E-5	0.0	0.0	0.10958059043645543	5.191156308359216E-4
36-37	1.8876932030397146E-5	0.0	0.0	0.1911950060698775	5.285540968511201E-4
38-39	1.8876932030397146E-5	0.0	0.0	0.3078827614157775	5.285540968511201E-4
40-41	1.8876932030397146E-5	0.0	0.0	0.44038938580315024	5.285540968511201E-4
42-43	1.8876932030397146E-5	0.0	0.0	0.5738398567920429	5.285540968511201E-4
44-45	1.8876932030397146E-5	0.0	0.0	0.7394094276306562	5.474310288815173E-4
46-47	1.8876932030397146E-5	0.0	0.0	0.9341249815242028	6.040618249727087E-4
48-49	1.8876932030397146E-5	0.0	0.0	1.135343638502221	6.41815689033503E-4
50-51	1.8876932030397146E-5	0.0	0.0	1.3454533304665566	6.41815689033503E-4
52-53	1.8876932030397146E-5	0.0	0.0	1.5590552548565153	6.795695530942972E-4
54-55	1.8876932030397146E-5	0.0	0.0	1.8035492785142193	7.267618831702902E-4
56-57	1.8876932030397146E-5	0.0	0.0	2.078973155303729	7.456388152006872E-4
58-59	1.8876932030397146E-5	0.0	0.0	2.381277783304524	7.928311452766801E-4
60-61	1.8876932030397146E-5	0.0	0.0	2.6955503862125907	8.117080773070773E-4
62-63	1.8876932030397146E-5	0.0	0.0	3.017817369835531	8.117080773070773E-4
64-65	1.8876932030397146E-5	0.0	0.0	3.343897493728611	8.117080773070773E-4
66-67	1.8876932030397146E-5	0.0	0.0	3.7106007753511063	8.40023475352673E-4
68-69	1.8876932030397146E-5	0.0	0.0	4.125968787747965	8.683388733982687E-4
70-71	1.8876932030397146E-5	0.0	0.0	4.571879676170006	9.06092737459063E-4
72-73	1.8876932030397146E-5	0.0	0.0	5.007908490674135	9.816004655806515E-4
74-75	1.8876932030397146E-5	0.0	0.0	5.450581985252963	0.0010004773976110487
76-77	1.8876932030397146E-5	0.0	0.0	5.919749253936454	0.0010004773976110487
78-79	1.8876932030397146E-5	0.0	0.0	6.4385817307919195	0.0010004773976110487
80-81	1.8876932030397146E-5	0.0	0.0	6.985899498081254	0.0010099158636262475
82-83	1.8876932030397146E-5	0.0	0.0	7.53921069129024	0.0010571081937022402
84-85	1.8876932030397146E-5	0.0	0.0	8.09479655480889	0.0010759851257326374
86-87	1.8876932030397146E-5	0.0	0.0	8.66313378591407	0.001085423591747836
88	1.8876932030397146E-5	0.0	0.0	9.105269287930033	0.0010948620577630344
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8805	0.0	43.43765	1
GTATCAA	14745	0.0	38.41376	1
TACCTGG	5940	0.0	38.39909	2
GTACCTG	7420	0.0	31.827679	1
ACCTGGG	7095	0.0	31.198578	3
TCAACGC	17795	0.0	31.11009	4
CAACGCA	18060	0.0	30.549517	5
ATCAACG	18125	0.0	30.518608	3
AACGCAG	18845	0.0	29.42603	6
TATCAAC	19010	0.0	29.117895	2
GTACATG	17005	0.0	26.890247	1
ACGCAGA	21510	0.0	25.77978	7
TACATGG	17520	0.0	25.17876	2
CGCAGAG	22330	0.0	24.819548	8
ACATGGG	17875	0.0	23.978117	3
CATGGGG	11935	0.0	22.562447	4
TATAACG	460	0.0	22.492125	2
ATAACGC	565	0.0	22.458544	3
GCAGAGT	25325	0.0	21.828432	9
CCTGGGG	6765	0.0	21.81302	4
>>END_MODULE