FastQCFastQC Report
Mon 6 Jun 2016
ERR522989_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522989_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6161052
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT624321.0133334372116969No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215050.3490475327914778No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT174730.2836041637045102No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163630.26558776001241347No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC134440.21820948760049422No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC121490.19719034995971468No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99760.1619203993084298No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG94910.15404836706458572No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA93100.15111055709317175No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC81600.13244491362838684No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG218950.028.2827011
TACCTGG46750.028.1587892
TACATGG225450.026.9223882
TATAACG5300.026.6123312
ACATGGG229350.025.912373
CATGGGG157150.024.7101254
ATGGGGG96900.023.9186125
TAACGCA5650.023.2980124
GTACCTG59650.022.9383741
ACCTGGG60750.021.3610023
ATAACGC7050.021.341243
GAGTACT180900.019.98593912-13
AGAGTAC279250.019.20429410-11
AGTACTT189300.018.37930112-13
TGGGGGG100000.018.0408696
GAGTACA171150.017.6924921
GTACTTT202050.017.63901114-15
GTATAGG17800.016.9059681
AGTACAT168700.016.6657052
TATCACG5650.016.642522
Produced by FastQC (version 0.11.5)