FastQCFastQC Report
Mon 6 Jun 2016
ERR522989_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522989_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6161052
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT649591.0543491598512722No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197770.32100037461134884No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT178320.28943109066438655No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159950.2596147541036823No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC141190.22916540876460706No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC129200.2097044465782792No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA102210.16589699291614485No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95930.15570392848494055No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA93330.15148386996246746No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC83230.13509056570209113No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG50700.030.22122
GTACATG225750.028.2319951
TACATGG232400.027.059642
ACATGGG238650.025.3627623
GTACCTG62550.025.1159251
ATGGGGG96500.024.59275
CATGGGG160250.024.545374
ACCTGGG64300.023.2411863
TGGGGGG94200.020.5536256
TATCACG6200.020.4679412
GAGTACT187050.020.28293212-13
TAACGCA5600.020.1402724
GTACTTT207100.018.13443214-15
AGAGTAC291800.017.8995410-11
GTATAGG17450.017.7901041
GAGTACA174450.017.7682421
TATAACG5300.017.736022
AGTACTT197600.017.37993412-13
AGTACAT169250.016.7452132
CCTGGGG63900.015.958834
Produced by FastQC (version 0.11.5)