FastQCFastQC Report
Mon 6 Jun 2016
ERR522988_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522988_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6791120
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126310.185992884826067No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100430.1478842959629634No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85350.045.8760641
GTATCAA156500.042.460661
TCAACGC184550.035.294174
CAACGCA190000.034.3802155
ATCAACG189900.034.2788853
AACGCAG198750.033.0725946
TATCAAC199050.032.848212
ACGCAGA231000.028.3131477
CGCAGAG241300.026.9289448
GTACATG233200.025.5286791
TACATGG239950.024.1148222
GCAGAGT272750.023.598349
ACATGGG241500.023.295873
CATGGGG172550.022.2243484
ATGGGGG105300.021.4666425
GTGGTAT52050.019.8915371
GAGTACT133900.019.73747612-13
AGAGTAC245050.019.5835610-11
CAGAGTA264950.019.13222710-11
GTACTTT146100.017.89582814-15
Produced by FastQC (version 0.11.5)