FastQCFastQC Report
Mon 6 Jun 2016
ERR522988_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522988_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6791120
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111650.16440587119650368No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94930.13978548457397308No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83700.047.218981
GTATCAA150050.039.979461
TCAACGC176750.033.288864
CAACGCA180400.032.563235
ATCAACG182550.032.2078323
TATCAAC190000.031.3168452
AACGCAG190900.030.9182826
ACGCAGA223250.026.4585387
GTACATG230250.025.6248111
CGCAGAG233500.025.2814258
TACATGG237400.024.2913072
ACATGGG241350.023.2121283
GCAGAGT272200.021.6697739
CATGGGG172000.021.6674
GTGGTAT51400.019.3144381
GAGTACT129850.019.18427812-13
TACCTGG55650.019.0177212
ATGGGGG102100.018.8717485
AGAGTAC238200.018.83413710-11
CAGAGTA255150.018.81717310-11
Produced by FastQC (version 0.11.5)