FastQCFastQC Report
Mon 6 Jun 2016
ERR522987_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522987_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4447035
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT333350.7496005765639353No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158110.355540264468348No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT153400.34494893788782865No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116480.26192732910804617No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT85790.19291505463752814No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73220.16464903019652422No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC67340.15142673714058918No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC58540.1316382713425912No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG47570.10697014977395052No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA47430.10665533327261871No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG45200.039.52482
GTACCTG52450.034.960921
ACCTGGG56500.031.4566153
GTACATG133850.028.4180261
ATAACGC4550.027.901083
TACATGG138150.027.1226372
ACATGGG135150.026.3359183
TATAACG5000.026.3276772
TAACGCA5150.024.64884
TATCACG5000.024.4471282
CATGGGG91000.024.2826274
CCTGGGG49900.022.7067894
ATGGGGG55900.022.287865
AGAGTAC189850.019.16394210-11
GAGTACT138100.018.87928812-13
ATCACGC7300.018.0344893
GTATAAC13400.017.5440141
GTACTTT153200.016.94400814-15
AGTACTT146150.016.714412-13
CATGGGA52900.016.708654
Produced by FastQC (version 0.11.5)