FastQCFastQC Report
Mon 6 Jun 2016
ERR522987_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522987_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4447035
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT335890.755312247373812No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143690.323114164831174No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113240.2546415757915105No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72170.16228790643653582No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC66430.14938042988193256No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT63050.14177986006406518No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC62180.1398235003772176No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG50270.11304161087106353No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA49570.1114675283644046No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA45270.10179816439492831No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG45750.043.3514372
GTACCTG55800.035.7296451
ACCTGGG58450.032.7233543
ATAACGC5100.030.408273
TAACGCA5250.028.6446464
GTACATG137800.026.4794861
TACATGG138250.025.93832
ACATGGG134800.025.2055593
TATAACG6150.024.4543972
CCTGGGG53450.023.6514284
TACCGGG4450.022.1789722
CATGGGG92750.021.9395034
AGAGTAC195700.019.59134310-11
ATGGGGG56650.019.4948885
GAGTACT143600.019.21818212-13
TATCACG3759.094947E-1218.7993182
AGTACTT150850.018.37245212-13
GTACTTT157400.017.4252614-15
GTATAGC15450.017.347761
CATGGGA54800.016.7227174
Produced by FastQC (version 0.11.5)