FastQCFastQC Report
Mon 6 Jun 2016
ERR522986_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522986_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21395731
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT870230.40673066977706906No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA768700.3592772782570504No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT690180.32257836855398864No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG431340.20160096422973348No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT415020.19397327438824127No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA367670.17184269142288244No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG367070.17156226164929816No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC341460.15959258414680946No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT340270.15903639842920067No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG299840.14014010551918044No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC254170.11879472591985756No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT253520.1184909269984746No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC244560.11430317571294946No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT236710.11063421950855523No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA236450.11051269994000205No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC233140.10896566235572881No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG233060.10892827171925092No Hit
GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG232400.10861979896830821No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG231920.1083954551494408No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC230020.10750742753309059No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT225000.10516116509410219No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT224300.10483399702492054No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG213970.10000593108971131No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACATAT68350.032.4821431
TACCTGG103850.030.1574632
CATATAG79800.029.346453
ATGGGAT362800.026.0750795
CATGGGG352350.025.2617074
GGATGAC176400.025.0683568
CATGGGT153350.024.8056774
ATGGGTA104850.023.3633045
TGGGTAT90450.023.1725776
ACCTGGG133200.023.1241743
ACTCCCG40300.022.8559386
GGATATG119350.022.188461
GAGTACT621200.022.00457412-13
CATTCCG30100.021.5444379
GTATAGG159050.021.322731
ATGGGCG27150.021.3010125
GATTGGC118750.021.2897839
ACCCTTG214500.021.1639087
GTACATG2038200.021.0792621
GTACTTT648650.020.94792414-15
Produced by FastQC (version 0.11.5)