FastQCFastQC Report
Mon 6 Jun 2016
ERR522986_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522986_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21395731
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT765010.3575526351495072No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA726470.3395396960262774No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT639170.29873716396976574No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG453800.21209838542090476No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT398910.18644373496750358No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA389430.18201294454487207No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG353930.1654208496078026No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT351790.1644206500820187No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC311050.1453794684556466No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG302210.1412478031248383No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT263000.12292171742110611No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC255590.11945840971734036No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC248910.11633629157143544No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG242760.11346188639219665No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG239800.11207843284251424No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC233380.10907783426516253No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC231930.10840012897900053No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT228270.10668950736013647No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA224340.10485269234315948No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT215790.1008565680695836No Hit
GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG215640.10078646062618754No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT215040.10050603085260325No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT214920.10044994489788642No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACATAT68100.028.7931561
TACCTGG102000.028.7064882
CATATAG79800.025.79343
CATGGGG343100.024.6814944
ATGGGAT354200.024.6375565
CATGGGT152300.024.0984134
GGATGAC176500.023.5429928
ATGGGTA104350.023.4177535
TGGGTAT88750.023.1392046
GGATATG112850.022.8755261
ACCTGGG126550.022.540523
GTATAGG157850.021.745961
GAGTACT645750.021.38716112-13
GATTGGC117650.021.0558519
GGGTATG94100.021.0246057
ACCCTTG219800.020.8241187
CATTCCG29550.020.6794979
GATATGA120600.020.6547872
GTACTTT668050.020.58613414-15
GTATGGT42600.020.5306951
Produced by FastQC (version 0.11.5)