FastQCFastQC Report
Mon 6 Jun 2016
ERR522985_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522985_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8564415
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253890.29644756822269824No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205700.2401798605041909No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115820.13523398854445984No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131400.049.5538331
GTATCAA236500.040.303691
TCAACGC281150.033.2120174
CAACGCA286750.032.5782785
ATCAACG287500.032.3993953
AACGCAG298050.031.4472376
TATCAAC299900.031.251482
ACGCAGA341400.027.371597
GTACATG282700.026.7939431
CGCAGAG350600.026.5439628
TACATGG287600.025.5441842
ACATGGG291250.024.6886063
GCAGAGT394900.023.2558639
TACCTGG63650.021.7105522
CATGGGG181600.021.5817014
GAGTACT232350.020.99889212-13
CAGAGTA380850.020.24013710-11
AGAGTAC352200.019.89265410-11
GTACTTT259650.018.70988514-15
ATGGGGG100350.017.8411465
Produced by FastQC (version 0.11.5)