FastQCFastQC Report
Mon 6 Jun 2016
ERR522985_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522985_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8564415
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233130.27220773397832776No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199720.23319748050508998No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115440.1347902921565571No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA142100.049.655731
GTATCAA246250.039.1033331
TCAACGC296700.031.757064
CAACGCA302300.031.1843155
ATCAACG303050.031.1230133
TATCAAC313950.030.1840572
AACGCAG315700.029.9489526
ACGCAGA362000.026.1184657
GTACATG290600.025.9969331
CGCAGAG371550.025.3252478
TACATGG299200.024.4574432
ACATGGG302500.023.5206363
GCAGAGT415450.022.4115859
TACCTGG69800.021.9646972
CATGGGG189300.021.126254
GAGTACT241900.020.6447712-13
CAGAGTA405800.019.75992210-11
AGAGTAC370300.019.74397510-11
GTACTTT265450.018.67895114-15
TGGTATC79500.017.7466832
Produced by FastQC (version 0.11.5)