FastQCFastQC Report
Mon 6 Jun 2016
ERR522984_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522984_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12548281
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT418700.3336712016570238No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT344380.2744439656714732No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209970.16732969240966153No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127080.10127283569757482No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG197150.049.468792
GTACCTG227300.043.642471
ACCTGGG233350.041.230693
CCTGGGG226950.030.3940074
TATAACG11050.026.8100452
GGTATCA403300.025.6115761
GTATCAA555800.025.464541
GTACATG561250.023.9769361
CATGGGG336800.022.9239314
TACATGG579350.022.8240952
TCAACGC640200.021.872434
ACATGGG596750.021.6702043
CAACGCA648000.021.5570645
ATCAACG651550.021.4209583
TATCAAC658250.021.3527742
AACGCAG665300.021.1193836
CTGGGGG143850.020.6244055
ATGGGGG194650.020.2902245
GAGTACT357100.019.55443612-13
TAACGCA15300.019.0540924
Produced by FastQC (version 0.11.5)