FastQCFastQC Report
Mon 6 Jun 2016
ERR522984_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522984_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12548281
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT378800.30187401764432914No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT331850.26445853420082No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206320.16442092745611928No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG199500.050.1072652
GTACCTG226950.044.5239331
ACCTGGG233750.042.057453
CCTGGGG225900.030.0180844
TATAACG12000.028.9818842
GGTATCA402550.025.9310721
GTATCAA557100.025.903061
GTACATG558600.022.112941
TCAACGC647100.022.0041264
CTGGGGG137200.021.8866655
CAACGCA656150.021.7077945
ATCAACG660350.021.5341433
TATCAAC666500.021.4926972
CATGGGG333900.021.4346494
TAACGCA14950.021.3747274
AACGCAG674750.021.2486086
TACATGG574000.021.2226312
ACATGGG588450.020.0605553
GAGTACT374100.019.50659612-13
ACGCAGA748350.019.1274937
Produced by FastQC (version 0.11.5)