##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR522984_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12548281 Sequences flagged as poor quality 0 Sequence length 100 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.23181980065636 33.0 31.0 34.0 30.0 34.0 2 32.36239712833973 34.0 31.0 34.0 30.0 34.0 3 32.55636784034403 34.0 31.0 34.0 30.0 35.0 4 35.650491649015514 37.0 35.0 37.0 33.0 37.0 5 35.5776293980028 36.0 35.0 37.0 33.0 37.0 6 35.69959287650635 37.0 35.0 37.0 33.0 37.0 7 35.53651667507287 37.0 35.0 37.0 33.0 37.0 8 35.524933494874716 36.0 35.0 37.0 33.0 37.0 9 36.820362884764855 38.0 37.0 39.0 33.0 39.0 10-11 36.623499465783404 37.5 35.0 39.0 33.0 39.0 12-13 36.627295802508726 37.5 35.0 39.0 33.0 39.0 14-15 37.813719106226586 38.0 37.0 41.0 33.0 41.0 16-17 37.626343480832155 38.0 36.5 41.0 33.0 41.0 18-19 37.51872670846309 38.0 36.0 41.0 32.0 41.0 20-21 37.53069651532349 38.0 36.5 41.0 33.0 41.0 22-23 37.38554806829717 38.0 36.0 41.0 33.0 41.0 24-25 37.30988324217476 38.0 36.5 41.0 32.0 41.0 26-27 37.00033494627671 38.0 36.0 40.0 32.5 41.0 28-29 36.96053451464786 38.0 36.0 40.0 32.0 41.0 30-31 36.725301258395476 38.0 35.5 40.0 31.5 41.0 32-33 36.69189975104956 37.5 36.0 40.0 30.5 41.0 34-35 36.46684378521648 38.0 35.0 40.0 30.5 41.0 36-37 36.2475077263571 37.0 35.5 40.0 30.5 41.0 38-39 36.204046633957276 37.0 35.0 40.0 30.0 41.0 40-41 36.39861463892943 37.5 35.5 40.0 30.5 41.0 42-43 36.44290680133797 37.5 35.5 40.0 30.5 41.0 44-45 36.264891581564044 37.0 35.0 40.0 30.5 41.0 46-47 36.16650097332057 37.0 35.5 40.0 30.0 41.0 48-49 35.955811915592264 38.0 35.0 40.0 30.0 41.0 50-51 35.790436514770434 38.0 34.5 40.0 29.0 41.0 52-53 35.78977606574159 37.5 35.0 40.0 28.5 41.0 54-55 35.584807353294046 37.5 34.0 40.0 28.0 41.0 56-57 35.330866833473046 37.0 34.5 40.0 28.0 41.0 58-59 35.13637091008721 37.0 34.0 39.5 27.5 41.0 60-61 34.94800407322724 36.5 34.0 39.0 27.5 41.0 62-63 34.70054603495092 36.5 34.0 39.0 26.0 41.0 64-65 34.50788888932277 36.5 33.5 38.5 26.0 40.5 66-67 34.00004195793831 35.5 33.5 38.0 26.0 40.0 68-69 33.86112093760094 35.5 33.0 37.0 26.0 40.0 70-71 33.445886133726205 35.0 33.0 37.0 25.5 39.0 72-73 33.21863070328119 35.0 33.0 37.0 25.0 39.0 74-75 33.031549142069736 35.0 32.5 37.0 24.5 39.0 76-77 31.449807547344534 34.0 30.0 36.0 23.0 37.0 78-79 32.56958618475311 35.0 32.0 36.5 24.0 37.0 80-81 32.687512895192576 35.0 32.0 36.0 25.0 37.5 82-83 32.59748944895321 35.0 32.0 37.0 24.5 37.0 84-85 32.27731997713472 35.0 32.0 36.0 24.0 36.5 86-87 32.16104576395763 35.0 32.0 36.0 24.0 36.0 88-89 32.12382393253706 35.0 32.0 36.0 23.5 37.0 90-91 31.897991127230895 34.5 32.0 35.0 23.5 36.5 92-93 31.69311334357272 35.0 31.5 35.0 22.0 36.5 94-95 31.83325170196619 35.0 31.0 35.0 22.0 36.5 96-97 31.562543666339636 35.0 31.0 35.0 21.0 37.0 98-99 31.414289574803114 34.0 31.0 35.0 20.5 36.0 100 30.171766794192767 34.0 29.0 35.0 19.0 36.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.07629289832880914 1101 2 -0.11812232915328735 1101 3 -0.11943604628960713 1101 4 -0.07606377335756065 1101 5 -0.13991134925525728 1101 6 -0.06999211961461071 1101 7 -0.069794495745235 1101 8 -0.18243424551596377 1101 9 -0.11099439454724802 1101 10-11 -0.10145813002399962 1101 12-13 -0.05336811025762955 1101 14-15 -0.2560335091328767 1101 16-17 -0.2566019074222865 1101 18-19 -0.378770324667542 1101 20-21 -0.2593208091495427 1101 22-23 -0.7965835662918508 1101 24-25 -0.3026035554754287 1101 26-27 -0.18749495317815246 1101 28-29 -0.24906286556405632 1101 30-31 -0.2594016732293838 1101 32-33 -0.20712285039726197 1101 34-35 -0.2820148549525996 1101 36-37 -0.29884262265714057 1101 38-39 -0.29637123798636367 1101 40-41 -0.2589611038795354 1101 42-43 -0.26125210536002896 1101 44-45 -0.3098444356688219 1101 46-47 -0.2042271848727566 1101 48-49 -0.3110867682128813 1101 50-51 -0.26104509123827313 1101 52-53 -0.31947914144021183 1101 54-55 -0.31120236259907585 1101 56-57 -0.44022271104667965 1101 58-59 -0.36494686976448065 1101 60-61 -0.5478075075661053 1101 62-63 -0.36706543696912775 1101 64-65 -0.357034594337037 1101 66-67 -0.39281652511314036 1101 68-69 -0.43023006615585757 1101 70-71 -0.5136863724331775 1101 72-73 -0.48836316832404947 1101 74-75 -0.4125307879869595 1101 76-77 -0.5114002724421773 1101 78-79 -0.42211798783318244 1101 80-81 -0.360348549007945 1101 82-83 -0.3388307841746325 1101 84-85 -0.3808819568279489 1101 86-87 -0.448359115064477 1101 88-89 -0.4841498848081329 1101 90-91 -0.501832190359508 1101 92-93 -0.429680327018783 1101 94-95 -0.48901190828733476 1101 96-97 -0.5551337680444242 1101 98-99 -0.5402629859605348 1101 100 -0.4999158256730283 1102 1 -0.023648931472536106 1102 2 -0.09874229877189578 1102 3 -0.1499416580812607 1102 4 -0.09389998643781183 1102 5 -0.12173302480721304 1102 6 -0.19146506659897256 1102 7 -0.08599118800054839 1102 8 -0.1918763428796666 1102 9 -0.1406343451160197 1102 10-11 -0.2587352324815555 1102 12-13 -0.16163032294661406 1102 14-15 -0.29293977847535757 1102 16-17 -0.32830946300301633 1102 18-19 -0.2636829220413972 1102 20-21 -0.3380485562763056 1102 22-23 -0.29597478925514054 1102 24-25 -0.21940893122868488 1102 26-27 -0.29623025823747895 1102 28-29 -0.3153560764667276 1102 30-31 -0.3749501551753269 1102 32-33 -0.2798660405075921 1102 34-35 -0.3667501161823239 1102 36-37 -0.37441430489044336 1102 38-39 -0.4352031632494189 1102 40-41 -0.37642698360898663 1102 42-43 -0.35853751673285217 1102 44-45 -0.26485431870793974 1102 46-47 -0.2843016898388626 1102 48-49 -0.4161195415251413 1102 50-51 -0.18417694829820164 1102 52-53 -0.3780217299091788 1102 54-55 -0.2799380578486961 1102 56-57 -0.38940544945563005 1102 58-59 -0.3304980910601074 1102 60-61 -0.36305706633037005 1102 62-63 -0.40415266374238 1102 64-65 -0.40510529040444965 1102 66-67 -0.5351081421151704 1102 68-69 -0.43363495753863646 1102 70-71 -0.44976664099099395 1102 72-73 -0.46643214862318416 1102 74-75 -0.4814541487333841 1102 76-77 -0.4427211280600112 1102 78-79 -0.4618829088704288 1102 80-81 -0.44316208294541326 1102 82-83 -0.40187997701523415 1102 84-85 -0.487192964149628 1102 86-87 -0.47296062654408644 1102 88-89 -0.4286877819069339 1102 90-91 -0.4675950097142305 1102 92-93 -0.48439848936146745 1102 94-95 -0.5620257194202658 1102 96-97 -0.5373248816475247 1102 98-99 -0.6248810832002327 1102 100 -0.729073303269157 1103 1 -0.08634852338667542 1103 2 -0.13032782422649802 1103 3 -0.2135377790657529 1103 4 -0.07692488013954346 1103 5 -0.1945902734619338 1103 6 -0.20058821626862056 1103 7 -0.15702992268025184 1103 8 -0.13236404551179248 1103 9 -0.23681450792950898 1103 10-11 -0.27903091777912437 1103 12-13 -0.22675844654691701 1103 14-15 -0.23745643052620835 1103 16-17 -0.309099667849388 1103 18-19 -0.35248887944447915 1103 20-21 -0.3447432767389955 1103 22-23 -0.44184810200336244 1103 24-25 -0.3060821255929227 1103 26-27 -0.38732638673710795 1103 28-29 -0.30546931325462623 1103 30-31 -0.4188745799049016 1103 32-33 -0.32981171286545674 1103 34-35 -0.4577492993547523 1103 36-37 -0.5019507266828285 1103 38-39 -0.3856481689458988 1103 40-41 -0.39569369571265156 1103 42-43 -0.435540342972665 1103 44-45 -0.3089487701012388 1103 46-47 -0.42235572316072734 1103 48-49 -0.4300910506666966 1103 50-51 -0.4238442980684738 1103 52-53 -0.3438226294852953 1103 54-55 -1.157854417874809 1103 56-57 -0.4344667033255831 1103 58-59 -0.4205926356411567 1103 60-61 -0.3975301961463771 1103 62-63 -0.31892538263043946 1103 64-65 -0.3119264633732257 1103 66-67 -0.3848017156306085 1103 68-69 -0.36325936578955265 1103 70-71 -0.47627027053113835 1103 72-73 -0.49682449503896464 1103 74-75 -0.5242437392510837 1103 76-77 -0.4066487965979917 1103 78-79 -0.33727309502458525 1103 80-81 -1.2180071548844502 1103 82-83 -0.27017368323440394 1103 84-85 -0.19939010735734541 1103 86-87 -0.2574592121944299 1103 88-89 -0.3469708234569211 1103 90-91 -0.2990939744009893 1103 92-93 -0.247736957130666 1103 94-95 -0.3180817758182606 1103 96-97 -0.3843455379024867 1103 98-99 -0.3404441217113714 1103 100 -0.40279784026127885 1104 1 -0.09859079961167083 1104 2 -0.20978258666148264 1104 3 -0.15823838987257233 1104 4 -0.03151903125201727 1104 5 -0.06731705155068113 1104 6 -0.23023839531299473 1104 7 -0.23695674582678805 1104 8 -0.09176218087403498 1104 9 -0.18235403883841883 1104 10-11 -0.18325505059342362 1104 12-13 -0.14198257725801255 1104 14-15 -0.3203486730985219 1104 16-17 -0.33062114990997316 1104 18-19 -0.2977512513752174 1104 20-21 -0.32660764381558494 1104 22-23 -0.25348553277268593 1104 24-25 -0.18558992298700616 1104 26-27 -0.2615274582964844 1104 28-29 -0.3685922841890985 1104 30-31 -0.3766753227302928 1104 32-33 -0.3133989342386698 1104 34-35 -0.3681463651679451 1104 36-37 -0.40755909063990003 1104 38-39 -0.28512347206514477 1104 40-41 -0.24025543886371992 1104 42-43 -0.2113123107579824 1104 44-45 -0.36439696118889486 1104 46-47 -0.28113655224874634 1104 48-49 -0.30939836053740777 1104 50-51 -0.2514220643517575 1104 52-53 -0.29029540386687813 1104 54-55 -0.2930499015409893 1104 56-57 -0.31711355080813775 1104 58-59 -0.3548565187944064 1104 60-61 -0.4304133457627586 1104 62-63 -0.369780756912931 1104 64-65 -0.5618683383800729 1104 66-67 -0.4597952072975531 1104 68-69 -0.35617936331924227 1104 70-71 -0.27787691308157747 1104 72-73 -0.33008533146471564 1104 74-75 -0.43583696306778563 1104 76-77 -0.327682606415145 1104 78-79 -0.26146037692099355 1104 80-81 -0.28901810120276394 1104 82-83 -0.309505531794656 1104 84-85 -0.9701140710820013 1104 86-87 -0.4210311440722663 1104 88-89 -0.36633179296651264 1104 90-91 -0.42449313645601094 1104 92-93 -0.394937129746598 1104 94-95 -0.3692438290912925 1104 96-97 -0.2953106130032275 1104 98-99 -0.30279987824489396 1104 100 -1.54034692180581 1105 1 -0.05178915660399497 1105 2 -0.1735402168146578 1105 3 -0.17267805559850302 1105 4 -0.04931407552115985 1105 5 -0.12585485862660306 1105 6 -0.12616012850408964 1105 7 -0.13330785693796088 1105 8 -0.17802232478153712 1105 9 -0.1199864583750454 1105 10-11 -0.15028068734057598 1105 12-13 -0.15128257986620497 1105 14-15 -0.2600689298432357 1105 16-17 -0.2302606942930865 1105 18-19 -0.3157318959807114 1105 20-21 -0.30075475575581123 1105 22-23 -0.2409990114961431 1105 24-25 -0.19394177569067494 1105 26-27 -0.25893266356032996 1105 28-29 -0.2072572335120384 1105 30-31 -0.30242782570110904 1105 32-33 -0.2270352547204979 1105 34-35 -0.31990935521246655 1105 36-37 -0.32824821395830384 1105 38-39 -0.2734702511824523 1105 40-41 -0.23990680300713052 1105 42-43 -0.2691098049024987 1105 44-45 -0.42260440105838626 1105 46-47 -0.4322233111854743 1105 48-49 -0.37732756651139 1105 50-51 -0.31406722818624644 1105 52-53 -0.45400696029209087 1105 54-55 -1.3591879013397303 1105 56-57 -0.45736000235738317 1105 58-59 -0.4583427600113197 1105 60-61 -0.3042265978727414 1105 62-63 -0.2967113740050564 1105 64-65 -0.2792568652381533 1105 66-67 -0.37989367741031543 1105 68-69 -0.15287741104728525 1105 70-71 -0.1971554420202537 1105 72-73 -0.2825312295749569 1105 74-75 -0.2788557550413415 1105 76-77 -0.3622932420433749 1105 78-79 -0.28893692731347187 1105 80-81 -0.18527894641957943 1105 82-83 -0.159036144503105 1105 84-85 -0.188905077339669 1105 86-87 -0.23940691594511065 1105 88-89 -0.31902949745211373 1105 90-91 -0.269812866740061 1105 92-93 -0.24429033002758516 1105 94-95 -0.277572276309332 1105 96-97 -0.3296248131802457 1105 98-99 -0.2699598033277155 1105 100 -0.2139812691010512 1106 1 -0.031887078532115254 1106 2 -0.13549648254066682 1106 3 -0.11000326852161635 1106 4 -0.06814399462968623 1106 5 -0.11529606197056097 1106 6 -0.10100097460318835 1106 7 -0.06334999383769002 1106 8 -0.13179887996160744 1106 9 -0.10914918217824976 1106 10-11 -0.18111357542634465 1106 12-13 -0.11850237202931169 1106 14-15 -0.1788670627317046 1106 16-17 -0.11802249031212142 1106 18-19 -0.24555004400264124 1106 20-21 -0.2016015678186136 1106 22-23 -0.1628379695192379 1106 24-25 -0.11144970208754046 1106 26-27 -0.10499110861579197 1106 28-29 -0.14488817130738596 1106 30-31 -0.19520683678045003 1106 32-33 -0.11920374969603387 1106 34-35 -0.13691061180789887 1106 36-37 -0.20457257260023454 1106 38-39 -0.24010329263992247 1106 40-41 -0.18016581189666425 1106 42-43 -0.21653594862289793 1106 44-45 -0.15134415169995208 1106 46-47 -0.26028625723760257 1106 48-49 -0.21216242033509758 1106 50-51 -0.14042025383972145 1106 52-53 -0.22513272295979192 1106 54-55 -0.22618659042339573 1106 56-57 -0.18533179113222076 1106 58-59 -0.2256410204266075 1106 60-61 -0.1794228215509719 1106 62-63 -0.26186467992047824 1106 64-65 -0.1762702761372239 1106 66-67 -0.1072814739632264 1106 68-69 -0.0922057924072277 1106 70-71 -0.0880047452060424 1106 72-73 -0.08338937264011292 1106 74-75 -0.12206948193533407 1106 76-77 -0.15494574481565238 1106 78-79 -0.03881476892303937 1106 80-81 -0.029199906100316753 1106 82-83 -0.05723447269221538 1106 84-85 0.04067354941923185 1106 86-87 0.04455207686034157 1106 88-89 -0.03186213345490785 1106 90-91 -0.04237843059323154 1106 92-93 0.0048100435559881305 1106 94-95 -0.040103028097998816 1106 96-97 0.03687242600193841 1106 98-99 -0.08274605436585958 1106 100 0.017804187069401678 1107 1 -0.028297592924403148 1107 2 -0.10402617762660782 1107 3 -0.09431009388227096 1107 4 -0.011409381546201303 1107 5 -0.1029888856269423 1107 6 -0.11013060850597611 1107 7 -0.16456752572354816 1107 8 -0.0656579233546637 1107 9 -0.15597129735385806 1107 10-11 -0.12400742043679003 1107 12-13 -0.15228975374574816 1107 14-15 -0.23874163568783047 1107 16-17 -0.13968603810338465 1107 18-19 -0.25558673955852385 1107 20-21 -0.17356255137990217 1107 22-23 -0.13178207611868942 1107 24-25 -0.06120070961090107 1107 26-27 -0.11878220718451615 1107 28-29 -0.1641368479949037 1107 30-31 -0.1798672090039375 1107 32-33 -0.04152535870132823 1107 34-35 -0.1584791202911262 1107 36-37 -0.23782492823897883 1107 38-39 -0.16498991192388246 1107 40-41 -0.2025090308786659 1107 42-43 -0.24708329891647907 1107 44-45 -0.2905633929556686 1107 46-47 -0.2942764733485106 1107 48-49 -0.24253851514635727 1107 50-51 -0.22903638246255298 1107 52-53 -0.1948350100979397 1107 54-55 -0.24940287157412655 1107 56-57 -0.2617948000913586 1107 58-59 -0.3024185922965046 1107 60-61 -0.3069689649026728 1107 62-63 -0.38385285510683786 1107 64-65 -0.38113844492817606 1107 66-67 -0.45511242629753923 1107 68-69 -0.3802984563695446 1107 70-71 -0.30297448725745113 1107 72-73 -0.33139353920923753 1107 74-75 -0.3441775232194644 1107 76-77 -0.30648268871904705 1107 78-79 -0.2825169257961804 1107 80-81 -0.06698129562659716 1107 82-83 -0.09800840605991823 1107 84-85 -0.08351190017099697 1107 86-87 -0.10017576562793096 1107 88-89 -0.0855042266956687 1107 90-91 -0.15890925060823946 1107 92-93 -0.014032104402442513 1107 94-95 0.0534987466910124 1107 96-97 0.004552548024253866 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2316 88-89 0.0844399220730736 2316 90-91 0.20021890571224787 2316 92-93 0.37925288276961666 2316 94-95 0.3074443639352751 2316 96-97 0.33084586398487303 2316 98-99 0.34859231788326284 2316 100 0.13508159593027713 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 3.0 10 4.0 11 12.0 12 89.0 13 417.0 14 1551.0 15 4134.0 16 8581.0 17 15758.0 18 25513.0 19 37377.0 20 50146.0 21 66598.0 22 86168.0 23 101695.0 24 115088.0 25 130152.0 26 151981.0 27 181552.0 28 220150.0 29 269223.0 30 331195.0 31 409041.0 32 513057.0 33 668299.0 34 945079.0 35 1684301.0 36 2589395.0 37 1912587.0 38 1721929.0 39 307042.0 40 162.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.41272777182869 17.661110296549957 12.642159192463472 24.284002739157877 2 16.861024689031947 19.95790530095446 38.43168872621454 24.749381283799057 3 19.597058752509607 24.94748882336951 29.132364823516465 26.323087600604417 4 13.640685857531507 15.853167554199937 35.416075947410086 35.09007064085847 5 14.340104465804357 36.17220321510117 33.36953501217423 16.11815730692024 6 32.578162106679166 34.48231151998521 17.885168325858206 15.054358047477423 7 29.07526768608925 31.33505149709761 21.01699993943393 18.57268087737921 8 27.410144215849925 33.767751644568804 19.735295866188448 19.08680827339282 9 27.488514163812557 15.43245644562789 19.4665866982099 37.61244269234965 10-11 26.05622634685978 25.01297986552899 27.42821108325515 21.50258270435608 12-13 26.250593110782177 22.76118510905827 27.69078642544942 23.29743535471013 14-15 23.73058516584129 24.165405719473156 25.38761124861867 26.71639786606688 16-17 23.49235236241337 26.881083909557958 26.074301696724707 23.552262031303968 18-19 23.31392708569546 25.99493627751408 27.86481826969333 22.82631836709713 20-21 23.98793512163299 25.277510850596556 27.810209275337467 22.924344752432987 22-23 23.873194331646 25.002480436824648 27.65778307681433 23.466542154715025 24-25 23.589252950087158 25.22877757298187 27.595980067299063 23.585989409631907 26-27 23.46843548240221 25.21200462528736 27.75545038466681 23.56410950764362 28-29 23.51398597915085 25.4191433998738 27.759757403618718 23.307113217356637 30-31 23.882821128801567 25.254550766931967 27.674721762307282 23.187906341959188 32-33 23.61727517304956 25.47311928821685 27.548932979592532 23.360672559141058 34-35 23.8000401525478 25.47432886945074 27.53419521837192 23.191435759629538 36-37 23.8780701108777 25.404168197663413 27.443166963448583 23.2745947280103 38-39 23.836338096875455 25.516246093280078 27.32736066538673 23.320055144457736 40-41 23.723069529686576 25.454987770559924 27.453578735039958 23.36836396471354 42-43 23.638812168731306 25.508238556099847 27.234021696119637 23.618927579049213 44-45 23.582126570154305 25.62094369789821 27.186044154977935 23.610885576969554 46-47 23.592931289050096 25.522045156802946 27.133551556954483 23.751471997192482 48-49 23.680371905923405 25.621457602097436 26.986844419384603 23.711326072594556 50-51 23.475753446243765 25.93983557057117 26.91750177007889 23.66690921310618 52-53 23.59390119655316 25.93565174324682 26.645151998650814 23.825295061549216 54-55 23.465665418187616 25.830665468794095 26.827298265249823 23.876370847768467 56-57 23.610099257690205 25.859984865068107 26.569699286173304 23.96021659106838 58-59 23.605106516197093 25.732840195079483 26.807105944187327 23.8549473445361 60-61 23.73044735556304 25.84637706315031 26.830583050678342 23.592592530608307 62-63 23.49889594289696 25.985861470558795 26.754326335499478 23.760916251044765 64-65 23.46610503693206 25.788107315577673 26.74869195836055 23.99709568912972 66-67 23.439362388801822 25.82429192641448 26.654565811043756 24.08177987373994 68-69 23.2715112851377 25.821670773207533 26.702190277324195 24.20462766433057 70-71 23.272131057919733 25.715443188966496 26.709972029175216 24.302453723938555 72-73 23.240104567318948 25.97957944747317 26.455511769999717 24.324804215208164 74-75 23.178637628134492 26.022732869614952 26.394406784172663 24.404222718077893 76-77 23.11707608626827 25.795967262052415 26.560011036391522 24.526945615287794 78-79 23.054926008696647 25.78774203595109 26.595599110156616 24.561732845195657 80-81 23.04530303966083 25.977745945159793 26.361842564017763 24.615108451161614 82-83 23.026848605211452 25.894277776978637 26.40693475571491 24.671938862095004 84-85 22.988286684495726 26.006506649278837 26.23538335912513 24.769823307100307 86-87 22.911396919288464 25.96501699939932 26.366089261539717 24.7574968197725 88-89 22.707676734576257 25.76821058423117 26.48090347563948 25.04320920555309 90-91 22.988247983042072 25.727658460807497 26.397199158542612 24.886894397607822 92-93 22.857973708561833 25.758980579690544 26.3503747680101 25.03267094373752 94-95 22.582554046552755 25.575295003589932 26.536813273883485 25.305337675973828 96-97 22.524760513652573 25.52393484774352 26.63198718430711 25.319317454296797 98-99 22.570415724866947 25.534591460610333 26.40900583006667 25.485986984456048 100 22.475257726947184 25.506817239456524 26.469117912035777 25.548807121560507 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3695.0 1 2839.5 2 5121.5 3 7161.5 4 4560.0 5 3405.0 6 4841.5 7 8652.5 8 13165.5 9 15166.5 10 13333.5 11 10877.5 12 10824.0 13 11570.0 14 12487.0 15 13491.5 16 14450.5 17 15461.5 18 16573.5 19 17462.0 20 18616.0 21 20386.0 22 22678.5 23 26155.5 24 30317.0 25 35519.5 26 43167.0 27 52275.5 28 62634.5 29 74895.5 30 89509.5 31 104368.5 32 120210.0 33 138190.5 34 157000.0 35 176518.0 36 201836.0 37 227581.5 38 247511.5 39 268339.0 40 287461.0 41 304637.0 42 325122.5 43 352292.0 44 379807.0 45 405969.5 46 435061.0 47 467611.5 48 508033.5 49 536498.5 50 554622.0 51 555039.0 52 534660.5 53 527621.5 54 520164.5 55 495708.5 56 459306.5 57 420964.0 58 386248.0 59 343864.0 60 292537.0 61 243648.0 62 201640.5 63 160406.0 64 121408.0 65 92644.5 66 70424.5 67 54535.0 68 42926.5 69 32760.0 70 25874.0 71 20499.0 72 16646.0 73 13875.0 74 11069.5 75 8422.0 76 5959.0 77 3774.5 78 2438.5 79 1672.0 80 1099.5 81 735.0 82 497.5 83 351.5 84 258.0 85 164.5 86 103.5 87 73.0 88 46.0 89 33.0 90 25.5 91 15.5 92 9.5 93 6.5 94 4.5 95 2.0 96 1.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04574331735159581 2 0.010622968994717284 3 0.0 4 1.514151619652126E-4 5 4.7815314304803976E-4 6 7.969219050800662E-6 7 7.969219050800662E-6 8 5.578453335560464E-5 9 0.0 10-11 0.0 12-13 2.789226667780232E-4 14-15 0.011921951699997792 16-17 0.0017771358483285478 18-19 1.992304762700166E-5 20-21 9.802139432484817E-4 22-23 7.012912764704583E-4 24-25 0.004450808839872171 26-27 0.01620540693980315 28-29 0.013388288005345113 30-31 0.010160754289770845 32-33 0.006885405259891774 34-35 0.009021155965506351 36-37 0.0050006849543774165 38-39 0.004068286325433739 40-41 0.0055067303641032584 42-43 0.006981035888501381 44-45 0.0072320662886016015 46-47 0.015751161453907512 48-49 0.018428819054976536 50-51 0.017771358483285482 52-53 0.01189007482379459 54-55 0.003825225144384318 56-57 0.003884994287265323 58-59 0.010300215623159859 60-61 0.0023748272771385976 62-63 1.3547672386361127E-4 64-65 2.988457144050249E-4 66-67 0.001055921524231088 68-69 5.578453335560464E-5 70-71 2.1915352389701825E-4 72-73 0.0030561955059820543 74-75 0.00576174537372888 76-77 0.0055904071641366656 78-79 0.0036259946681143017 80-81 4.781531430480398E-5 82-83 9.921677718246827E-4 84-85 3.2275337155742687E-4 86-87 3.4666102870982885E-4 88-89 2.3110735247321924E-4 90-91 4.1439939064163447E-4 92-93 4.1041478111623414E-4 94-95 1.1953828576200995E-5 96-97 0.0018129973340571509 98-99 4.3033782874323586E-4 100 1.5938438101601327E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.2548281E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.8970323703329 #Duplication Level Percentage of deduplicated Percentage of total 1 66.16154886619991 31.68941847715739 2 17.550968887403382 16.81278649861331 3 6.889786738207682 9.900010152738709 4 3.29041583761408 6.304046155442307 5 1.8128019103378086 4.3413915890225665 6 1.1010865017156883 3.164326548912776 7 0.6937239264358507 2.325912215240067 8 0.4806589938967373 1.8417711511810935 9 0.338448382762851 1.4589605830391186 >10 1.5555180439842897 12.838520429860415 >50 0.0827457689257951 2.71112755267095 >100 0.03864965523408862 3.3202584989085064 >500 0.0024397548322025384 0.8062897612103782 >1k 9.499809033691128E-4 0.75512420071888 >5k 1.5405092772370765E-4 0.4591691747779872 >10k+ 1.0270061848247177E-4 1.2708870105055523 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 37880 0.30187401764432914 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 33185 0.26445853420082 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20632 0.16442092745611928 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 7.969219050800664E-6 0.0 2 0.0 0.0 0.0 7.969219050800664E-6 0.0 3 0.0 0.0 0.0 2.390765715240199E-5 0.0 4 0.0 0.0 0.0 3.984609525400332E-5 0.0 5 0.0 0.0 0.0 4.781531430480398E-5 0.0 6 0.0 0.0 0.0 4.781531430480398E-5 2.390765715240199E-5 7 0.0 0.0 0.0 4.781531430480398E-5 3.984609525400332E-5 8 0.0 0.0 0.0 4.781531430480398E-5 3.984609525400332E-5 9 0.0 0.0 0.0 7.172297145720597E-5 3.984609525400332E-5 10-11 0.0 0.0 0.0 7.969219050800664E-5 3.984609525400332E-5 12-13 0.0 0.0 0.0 9.96152381350083E-5 3.984609525400332E-5 14-15 7.969219050800664E-6 0.0 0.0 1.275075048128106E-4 9.96152381350083E-5 16-17 7.969219050800664E-6 0.0 0.0 1.4743055243981226E-4 1.514151619652126E-4 18-19 1.1953828576200997E-5 0.0 0.0 1.7930742864301493E-4 1.7532281911761458E-4 20-21 1.5938438101601328E-5 0.0 0.0 2.390765715240199E-4 1.7930742864301493E-4 22-23 1.5938438101601328E-5 0.0 0.0 4.383070477940365E-4 1.9126125721921592E-4 24-25 1.992304762700166E-5 3.984609525400332E-6 0.0 8.726294860626726E-4 2.151689143716179E-4 26-27 2.390765715240199E-5 7.969219050800664E-6 0.0 0.0019165971817175593 2.151689143716179E-4 28-29 2.390765715240199E-5 1.1953828576200997E-5 0.0 0.005092330973461624 2.271227429478189E-4 30-31 2.390765715240199E-5 1.5938438101601328E-5 0.0 0.015101670101267256 2.390765715240199E-4 32-33 5.578453335560464E-5 1.5938438101601328E-5 0.0 0.03881009677739923 2.5501500962562125E-4 34-35 7.172297145720597E-5 1.5938438101601328E-5 0.0 0.07737713237374905 3.028303239304252E-4 36-37 7.172297145720597E-5 1.5938438101601328E-5 0.0 0.13773201285498787 3.107995429812259E-4 38-39 7.172297145720597E-5 1.5938438101601328E-5 0.0 0.2235047174987554 3.386918096590282E-4 40-41 7.172297145720597E-5 1.5938438101601328E-5 0.0 0.3185336700700279 3.426764191844285E-4 42-43 7.172297145720597E-5 3.5861485728602984E-5 0.0 0.41126748755466985 3.4666102870982885E-4 44-45 7.172297145720597E-5 3.984609525400332E-5 0.0 0.5257532884384721 3.5064563823522917E-4 46-47 7.172297145720597E-5 3.984609525400332E-5 0.0 0.6652146218274838 3.8252251443843184E-4 48-49 7.969219050800664E-5 3.984609525400332E-5 0.0 0.809130748665893 4.2236860969243516E-4 50-51 8.766140955880729E-5 3.984609525400332E-5 0.0 0.955314118324255 4.2236860969243516E-4 52-53 8.766140955880729E-5 3.984609525400332E-5 0.0 1.1058128200986255 4.5823009542103816E-4 54-55 8.766140955880729E-5 3.984609525400332E-5 0.0 1.2768003840526045 4.8612236209884046E-4 56-57 8.766140955880729E-5 3.984609525400332E-5 0.0 1.4771067048944793 5.020608002004419E-4 58-59 8.766140955880729E-5 3.984609525400332E-5 0.0 1.6983919948875865 5.179992383020431E-4 60-61 8.766140955880729E-5 5.578453335560464E-5 0.0 1.9243193549777855 5.419068954544451E-4 62-63 8.766140955880729E-5 5.578453335560464E-5 0.0 2.155402799793852 5.658145526068471E-4 64-65 8.766140955880729E-5 5.578453335560464E-5 0.0 2.3968263063283333 5.777683811830481E-4 66-67 8.766140955880729E-5 5.578453335560464E-5 0.0 2.669548920684833 5.937068192846495E-4 68-69 8.766140955880729E-5 5.578453335560464E-5 0.0 2.982029968885778 6.056606478608504E-4 70-71 8.766140955880729E-5 5.578453335560464E-5 0.0 3.3130713282560373 6.295683050132524E-4 72-73 8.766140955880729E-5 5.578453335560464E-5 0.0 3.6326290429740933 7.411373717244618E-4 74-75 9.164601908420763E-5 5.578453335560464E-5 0.0 3.954202970112002 7.491065907752624E-4 76-77 9.563062860960796E-5 5.578453335560464E-5 0.0 4.3121563822168145 7.530912003006628E-4 78-79 9.563062860960796E-5 5.578453335560464E-5 0.0 4.7158650655018 7.57075809826063E-4 80-81 9.563062860960796E-5 5.976914288100498E-5 0.0 5.135376710164524 7.889526860292656E-4 82-83 9.563062860960796E-5 6.375375240640531E-5 0.0 5.5685954115946235 8.52706438435671E-4 84-85 9.563062860960796E-5 6.375375240640531E-5 0.0 5.996136044451029 8.52706438435671E-4 86-87 9.563062860960796E-5 6.375375240640531E-5 0.0 6.4421772193338676 8.52706438435671E-4 88 9.563062860960796E-5 6.375375240640531E-5 0.0 6.797409143132833 8.686448765372724E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 19950 0.0 50.107265 2 GTACCTG 22695 0.0 44.523933 1 ACCTGGG 23375 0.0 42.05745 3 CCTGGGG 22590 0.0 30.018084 4 TATAACG 1200 0.0 28.981884 2 GGTATCA 40255 0.0 25.931072 1 GTATCAA 55710 0.0 25.90306 1 GTACATG 55860 0.0 22.11294 1 TCAACGC 64710 0.0 22.004126 4 CTGGGGG 13720 0.0 21.886665 5 CAACGCA 65615 0.0 21.707794 5 ATCAACG 66035 0.0 21.534143 3 TATCAAC 66650 0.0 21.492697 2 CATGGGG 33390 0.0 21.434649 4 TAACGCA 1495 0.0 21.374727 4 AACGCAG 67475 0.0 21.248608 6 TACATGG 57400 0.0 21.222631 2 ACATGGG 58845 0.0 20.060555 3 GAGTACT 37410 0.0 19.506596 12-13 ACGCAGA 74835 0.0 19.127493 7 >>END_MODULE