FastQCFastQC Report
Mon 6 Jun 2016
ERR522983_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522983_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8750802
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279140.31898790533713367No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220120.2515426586043199No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129460.14794072589003843No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA188000.035.8901371
GTATCAA297950.034.742671
TCAACGC344300.029.5224
CAACGCA350650.028.8657745
ATCAACG353050.028.766673
TATCAAC363100.028.0777032
AACGCAG363850.027.9150056
GTACATG296100.027.2050291
TACCTGG75150.027.0824012
TACATGG305150.025.6927722
ACGCAGA409350.024.7665797
ACATGGG316150.024.171213
CGCAGAG423550.023.8905168
CATGGGG232550.023.1172374
GTACCTG93850.022.5110571
GCAGAGT462500.021.409979
GAGTACT234550.020.75974812-13
ACCTGGG94650.020.755193
ATGGGGG133300.020.69285
AGAGTAC368000.020.11003510-11
Produced by FastQC (version 0.11.5)