FastQCFastQC Report
Mon 6 Jun 2016
ERR522983_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522983_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8750802
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254500.29083048616572515No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216760.24770301053549149No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125130.14299260799181607No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA183100.038.923431
GTATCAA292100.034.271011
TCAACGC347950.028.3893744
ATCAACG354150.027.9201853
CAACGCA354650.027.91935
TACCTGG78150.027.5021922
TATCAAC364700.027.1969912
AACGCAG367800.026.9846746
GTACATG303050.026.6062321
TACATGG317100.024.9465082
ACATGGG324950.023.7040313
ACGCAGA419000.023.6532127
CGCAGAG436600.022.7051648
GTACCTG98450.022.3145451
CATGGGG240550.022.0756284
ACCTGGG94650.021.797353
GCAGAGT477300.020.5918059
GAGTACT249400.020.4774112-13
AGAGTAC386250.020.08572610-11
ATGGGGG134550.019.5588935
Produced by FastQC (version 0.11.5)