##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR522983_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8750802
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.673984510219746	34.0	31.0	34.0	30.0	35.0
2	32.734907954722324	34.0	31.0	34.0	31.0	34.0
3	33.00888935665554	34.0	31.0	34.0	31.0	36.0
4	35.794231545862885	37.0	35.0	37.0	33.0	37.0
5	35.849127771374555	37.0	35.0	37.0	35.0	37.0
6	35.92408352971533	37.0	35.0	37.0	35.0	37.0
7	35.749168590490335	37.0	35.0	37.0	35.0	37.0
8	35.79938170238568	37.0	36.0	37.0	34.0	37.0
9	37.01548475214043	39.0	36.0	39.0	34.0	39.0
10-11	37.13774926001068	38.5	36.0	39.0	34.0	39.0
12-13	37.00479984577414	38.0	36.5	39.0	33.5	39.0
14-15	38.10518961576322	39.0	36.5	41.0	34.5	41.0
16-17	38.12574887421747	39.0	37.0	41.0	34.5	41.0
18-19	38.12629533841584	39.0	37.0	41.0	34.0	41.0
20-21	38.01315833680158	39.0	36.5	41.0	34.0	41.0
22-23	37.930480371970475	39.0	36.5	41.0	34.0	41.0
24-25	37.796248846677145	39.0	36.0	41.0	34.0	41.0
26-27	37.67031621787352	39.0	36.5	41.0	34.0	41.0
28-29	37.63427460705887	39.0	36.5	41.0	34.5	41.0
30-31	37.481429016449006	38.0	36.0	41.0	34.0	41.0
32-33	37.341398765507435	38.0	36.5	41.0	33.0	41.0
34-35	37.16755309970446	38.0	36.0	41.0	33.0	41.0
36-37	37.18827862863313	38.0	35.5	41.0	33.0	41.0
38-39	37.328145351706056	38.0	36.0	41.0	33.0	41.0
40-41	37.22970865984627	38.0	36.5	41.0	33.0	41.0
42-43	37.2579626415956	38.0	36.5	41.0	33.0	41.0
44-45	37.09535114610067	38.0	36.0	41.0	33.0	41.0
46-47	36.832177667829754	38.0	36.0	41.0	32.0	41.0
48-49	36.79552239897555	38.0	36.5	40.5	32.0	41.0
50-51	36.403961145504155	38.0	35.0	40.0	31.0	41.0
52-53	36.45266302448621	38.0	35.5	40.0	31.0	41.0
54-55	36.2552552326061	37.5	35.5	40.0	31.0	41.0
56-57	35.956830528219015	37.0	35.0	40.0	29.5	41.0
58-59	35.8258943580257	36.5	35.0	40.0	30.0	41.0
60-61	35.55482440352324	36.5	35.0	40.0	29.0	41.0
62-63	35.369012805911964	37.5	34.5	39.5	28.5	41.0
64-65	35.225909008111486	37.0	35.0	39.0	28.0	41.0
66-67	34.99707901058669	36.5	34.0	39.0	28.5	41.0
68-69	34.525508519104875	36.0	34.0	38.0	27.0	40.0
70-71	34.28118131343847	36.0	34.0	37.5	26.5	40.0
72-73	34.016183545233915	36.0	34.0	37.0	26.0	39.0
74-75	33.738919701302805	35.5	33.5	37.0	26.0	39.0
76-77	32.27185131145694	34.5	31.0	36.0	24.5	37.5
78-79	33.27592659507094	35.0	33.0	37.0	26.0	37.0
80-81	33.27461625802984	35.0	33.0	36.5	26.0	37.0
82-83	33.09643178990909	35.0	33.0	36.0	26.0	37.5
84-85	32.89998510993621	35.0	33.0	36.0	25.5	37.0
86-87	32.65975724282186	35.0	33.0	36.0	25.0	37.0
88-89	32.50055629186902	35.0	33.0	36.0	25.0	36.5
90-91	32.36306906498399	35.0	32.5	35.5	25.0	37.0
92-93	32.21641045015074	35.0	32.0	35.5	24.0	36.5
94-95	32.095172533900325	35.0	32.0	35.0	24.5	36.0
96-97	31.91720861699305	35.0	32.0	35.0	23.5	36.5
98-99	31.790767291957927	35.0	31.5	35.0	23.0	36.5
100	30.614310436917666	34.0	29.0	35.0	19.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.13671697668864624
1101	2	-0.15956045076077174
1101	3	-0.18834766815691495
1101	4	-0.1492837471479831
1101	5	-0.16965472770576895
1101	6	-0.22665830399274256
1101	7	-0.18034891856746782
1101	8	-0.11178633913740299
1101	9	-0.16970477009726181
1101	10-11	-0.11743840310580111
1101	12-13	-0.16144611121134034
1101	14-15	-0.31521489013564263
1101	16-17	-0.1983818475303849
1101	18-19	-0.21176433405613437
1101	20-21	-0.24092595328064448
1101	22-23	-0.17083911323776135
1101	24-25	-0.276257167153112
1101	26-27	-0.28630203065078774
1101	28-29	-0.3126057104574471
1101	30-31	-0.3312233146841166
1101	32-33	-0.2619067715888903
1101	34-35	-0.23818811473463342
1101	36-37	-0.41074068716507384
1101	38-39	-0.27696190501277584
1101	40-41	-0.31494856632316015
1101	42-43	-0.33752613221528094
1101	44-45	-0.38461912738228676
1101	46-47	-0.3908382290540686
1101	48-49	-0.489644744458694
1101	50-51	-0.3605749157212088
1101	52-53	-0.5507879379967378
1101	54-55	-0.4455318990099997
1101	56-57	-0.4943509443752916
1101	58-59	-0.6423282620052007
1101	60-61	-0.6213518467254389
1101	62-63	-0.4666393620620539
1101	64-65	-0.5518262631054611
1101	66-67	-0.577947991112751
1101	68-69	-0.5349494104937165
1101	70-71	-0.5142603468885056
1101	72-73	-0.6940459352418955
1101	74-75	-0.6640884480897071
1101	76-77	-0.5035680825143345
1101	78-79	-0.5840167804161496
1101	80-81	-0.3199667685364531
1101	82-83	-0.5293684295965946
1101	84-85	-0.6046036660284742
1101	86-87	-0.5495331709848372
1101	88-89	-0.5653271403713838
1101	90-91	-0.6349353145683061
1101	92-93	-0.8572962092156899
1101	94-95	-0.7495648567646143
1101	96-97	-0.7305121486503907
1101	98-99	-0.7670190278231033
1101	100	-0.7304151081901438
1102	1	-0.02500682966596912
1102	2	-0.054183325781338
1102	3	-0.0885957902791148
1102	4	-0.03402700502277156
1102	5	-0.09674763505504558
1102	6	-0.1016059496449131
1102	7	-0.08960346904709127
1102	8	-0.09106425942699303
1102	9	-0.11309368607964387
1102	10-11	-0.07313890782895527
1102	12-13	-0.07683683395533336
1102	14-15	-0.19112281224878558
1102	16-17	-0.06400430447332894
1102	18-19	-0.1208745091020873
1102	20-21	-0.14783431507769507
1102	22-23	-0.06198706032063228
1102	24-25	-0.08957356591599819
1102	26-27	-0.0914956549678152
1102	28-29	-0.1023639811193604
1102	30-31	-0.0669109437903117
1102	32-33	-0.092821282335386
1102	34-35	-0.121132662656251
1102	36-37	-0.18363827196741767
1102	38-39	-0.20429997207016726
1102	40-41	-0.18479349090944908
1102	42-43	-0.2641307820750498
1102	44-45	-0.34032408721927254
1102	46-47	-0.2463742136574112
1102	48-49	-0.25143073455487297
1102	50-51	-0.17114065014144586
1102	52-53	-0.22460348717144285
1102	54-55	-0.30328581369282404
1102	56-57	-0.2620897777879918
1102	58-59	-0.3732381499795707
1102	60-61	-0.26256952411410595
1102	62-63	-0.24310022667241782
1102	64-65	-0.2454882288901601
1102	66-67	-0.2908838541989809
1102	68-69	-0.2969705210809863
1102	70-71	-0.2833026424952152
1102	72-73	-0.48600177361176833
1102	74-75	-0.7591679932343425
1102	76-77	-0.33859673876641594
1102	78-79	-0.3133701030001461
1102	80-81	-0.25440764587018094
1102	82-83	-0.24126543315194482
1102	84-85	-0.27266726439402333
1102	86-87	-0.41451709316185514
1102	88-89	-0.3030318814142774
1102	90-91	-0.30834648272875853
1102	92-93	-0.350309536117436
1102	94-95	-0.4049840511337379
1102	96-97	-0.46425701010926446
1102	98-99	-0.5945609295206857
1102	100	-0.4391652802934196
1103	1	-0.1074257383466275
1103	2	-0.078726085545199
1103	3	-0.131119038542586
1103	4	-0.05841534386271263
1103	5	-0.16702219330474577
1103	6	-0.09521962972000608
1103	7	-0.06385983042761723
1103	8	-0.0627873680776787
1103	9	-0.07099012143230254
1103	10-11	-0.10591703155531462
1103	12-13	-0.08889344794345533
1103	14-15	-0.16295309452289786
1103	16-17	-0.17892700350387258
1103	18-19	-0.17604988669572919
1103	20-21	-0.13626111872246582
1103	22-23	-0.06050106294527069
1103	24-25	-0.1571517187323508
1103	26-27	-0.1708162571272851
1103	28-29	-0.1514703532531314
1103	30-31	-0.1609488607656786
1103	32-33	-0.10028143172934989
1103	34-35	-0.06184365996981711
1103	36-37	-0.17072203620809745
1103	38-39	-0.16490200315386971
1103	40-41	-0.2474871699542618
1103	42-43	-0.24768192347014661
1103	44-45	-0.3951768275507348
1103	46-47	-0.327283262085615
1103	48-49	-0.3334579491594525
1103	50-51	-0.21069381746889349
1103	52-53	-0.25764673404102467
1103	54-55	-0.22997157943281366
1103	56-57	-0.13687139358825107
1103	58-59	-0.23063568972291648
1103	60-61	-0.31718086463046546
1103	62-63	-0.32388272410332064
1103	64-65	-0.3865317275858544
1103	66-67	-0.341969848352754
1103	68-69	-0.2312974113178896
1103	70-71	-0.34499872569001866
1103	72-73	-0.41555638746417145
1103	74-75	-0.3589000073433013
1103	76-77	-0.27075240622165353
1103	78-79	-0.2973633754630072
1103	80-81	-0.2653262976610833
1103	82-83	-0.29749789433599716
1103	84-85	-0.2753106412116253
1103	86-87	-0.30423196587045
1103	88-89	-0.2990901009334479
1103	90-91	-0.28472482603346805
1103	92-93	-0.3494930748549194
1103	94-95	-0.0844363955128955
1103	96-97	-0.822645048639778
1103	98-99	-1.2138995964961126
1103	100	-0.4146954049728677
1104	1	-0.0032938781348264
1104	2	-0.05307422958343011
1104	3	-0.04405070905945507
1104	4	-0.11979052411378888
1104	5	-0.1307663843540965
1104	6	-0.07492295504324176
1104	7	-0.11409956565516666
1104	8	-0.06035126365621579
1104	9	-0.06637937045938713
1104	10-11	-0.09959334440933532
1104	12-13	-0.07450042050272287
1104	14-15	-0.15098598715380263
1104	16-17	-0.0950746653796557
1104	18-19	-0.09420068908353585
1104	20-21	-0.1878112402954102
1104	22-23	-0.15144702903349128
1104	24-25	-0.10267914536512279
1104	26-27	-0.05414120872084993
1104	28-29	-0.17502510666044913
1104	30-31	-0.1562689435650384
1104	32-33	-0.0853151425228802
1104	34-35	-0.032523631486988336
1104	36-37	-0.09686550840513775
1104	38-39	-0.16179799993808075
1104	40-41	-0.16674244820698192
1104	42-43	-0.1923988704109192
1104	44-45	-0.22730461576961858
1104	46-47	-0.17028741976206163
1104	48-49	-0.18563820603694126
1104	50-51	-0.06790176823397331
1104	52-53	-0.1082045227419286
1104	54-55	-0.2218198709753736
1104	56-57	-0.12080676294980464
1104	58-59	-0.11736231342393921
1104	60-61	-0.16911161486327586
1104	62-63	-0.042612759899022024
1104	64-65	-0.25486775808442985
1104	66-67	-0.31420812465730563
1104	68-69	-0.180556796662799
1104	70-71	-0.2018138742215143
1104	72-73	-0.17918828861189695
1104	74-75	-0.200248860752275
1104	76-77	-0.1537274370546271
1104	78-79	-0.17062859157650223
1104	80-81	-0.043588126535262006
1104	82-83	-0.06834196292697214
1104	84-85	-0.08607644793799096
1104	86-87	-0.13510060713365846
1104	88-89	-0.0881386253700498
1104	90-91	-0.09986561504145186
1104	92-93	-0.20941671327484812
1104	94-95	-0.24797521607068518
1104	96-97	0.014094542736316384
1104	98-99	-0.9689798003355783
1104	100	-0.2615520857991527
1105	1	-0.09276936324206275
1105	2	-1.0353999207405487
1105	3	-0.15137146889084363
1105	4	-0.08427953059801041
1105	5	-0.12113246953792611
1105	6	-0.09367121913705034
1105	7	-0.10240419761230157
1105	8	-0.1146513637780302
1105	9	-0.1567909457973755
1105	10-11	-0.0847351407035859
1105	12-13	-0.0766015496914747
1105	14-15	-0.15039911299604114
1105	16-17	-0.5928036241816557
1105	18-19	-0.11152063842768456
1105	20-21	-0.22755058049251176
1105	22-23	-0.17465606830201352
1105	24-25	-0.19468818522752684
1105	26-27	-0.15352497329066495
1105	28-29	-0.20008339970272715
1105	30-31	-0.12411943064745401
1105	32-33	-0.08905962275213852
1105	34-35	-0.11924827198538424
1105	36-37	-0.2028955268695043
1105	38-39	-0.1525661207307465
1105	40-41	-0.15425851213308306
1105	42-43	-0.22527770254148294
1105	44-45	-0.2663838874138733
1105	46-47	-0.21471589395791568
1105	48-49	-0.16888661186659704
1105	50-51	-0.09220875656942695
1105	52-53	-0.10742510152473272
1105	54-55	-0.2654085881233357
1105	56-57	-0.09271089256922238
1105	58-59	-0.18426709419046006
1105	60-61	-0.15039363467702316
1105	62-63	-0.05537937228928769
1105	64-65	-0.08489760029367233
1105	66-67	-0.15876147672331342
1105	68-69	-0.15041211951987776
1105	70-71	-0.19450414491736012
1105	72-73	-0.23530171806471856
1105	74-75	-0.1614753644433975
1105	76-77	-0.1944633292475686
1105	78-79	-0.15339486089497
1105	80-81	-0.0997101659237245
1105	82-83	-0.07441772739115748
1105	84-85	0.0024887966297839625
1105	86-87	-0.11846766304630307
1105	88-89	-0.08298652340041457
1105	90-91	-0.09138696269747726
1105	92-93	-0.12275442355744559
1105	94-95	-0.1750637094000851
1105	96-97	-0.12053091459112153
1105	98-99	-0.13262131740013672
1105	100	-0.05176772312846367
1106	1	-0.06541267060863731
1106	2	-0.11298570111536321
1106	3	-0.07930405054869993
1106	4	-0.03572138101367983
1106	5	-0.03937846128933842
1106	6	-0.045169140342750325
1106	7	-0.04034637126417806
1106	8	0.012767015828941908
1106	9	-0.01773076923642236
1106	10-11	-0.0774004284441574
1106	12-13	-0.016544609030312074
1106	14-15	-0.06703469208829205
1106	16-17	0.025568518393129125
1106	18-19	-0.036161750254088076
1106	20-21	-0.051462403966105796
1106	22-23	0.034609517244561516
1106	24-25	0.060011934764752084
1106	26-27	0.013812062960582239
1106	28-29	-0.0410820908380245
1106	30-31	0.0024881531096312415
1106	32-33	0.06790090965477447
1106	34-35	0.042249064970746986
1106	36-37	-0.019389104182266692
1106	38-39	-0.028720078659986825
1106	40-41	-0.018580707456813172
1106	42-43	-0.021970805764077284
1106	44-45	-0.05472566852079552
1106	46-47	-0.024347914018228778
1106	48-49	-0.04574382834765345
1106	50-51	0.08433140046935961
1106	52-53	0.0281283225054878
1106	54-55	-0.044023667443411796
1106	56-57	-0.07763815115683315
1106	58-59	-0.08643853600914042
1106	60-61	0.046151529323331886
1106	62-63	0.05040723859394092
1106	64-65	-0.04583632208672128
1106	66-67	-0.02107935265825489
1106	68-69	-0.018501872635674488
1106	70-71	-0.01695856356614911
1106	72-73	-0.015686161824270073
1106	74-75	-0.05432670549645735
1106	76-77	-0.12014362361779263
1106	78-79	-0.04783231206226901
1106	80-81	0.02795460780208714
1106	82-83	0.00942094106505209
1106	84-85	0.15273823685834742
1106	86-87	0.10333816574578947
1106	88-89	0.06409620482967426
1106	90-91	0.07092732866086493
1106	92-93	0.0592583518423524
1106	94-95	0.009953093367762733
1106	96-97	-0.09350840958909856
1106	98-99	-0.07260000789863597
1106	100	-0.02482139688246221
1107	1	-0.05830317176911848
1107	2	-0.10364499066972144
1107	3	-0.07015154677922908
1107	4	-0.05948005158319347
1107	5	-0.060343022175253225
1107	6	-0.08318282820849277
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2316	80-81	-0.07478054833193681
2316	82-83	-0.08455664523217621
2316	84-85	-0.02916475025273968
2316	86-87	-0.1646206853418235
2316	88-89	-0.1066224439298935
2316	90-91	-0.17181924499195844
2316	92-93	-0.2574176111151161
2316	94-95	-0.10071811156250732
2316	96-97	0.008189236830205004
2316	98-99	0.10697076543632278
2316	100	-0.10003566637956851
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	7.0
12	33.0
13	146.0
14	647.0
15	1714.0
16	3820.0
17	7511.0
18	12643.0
19	18935.0
20	27000.0
21	37727.0
22	49434.0
23	58468.0
24	63482.0
25	69797.0
26	79555.0
27	93788.0
28	113653.0
29	140099.0
30	173636.0
31	218653.0
32	280464.0
33	376144.0
34	570816.0
35	1090125.0
36	1906402.0
37	1458620.0
38	1569604.0
39	327705.0
40	173.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.87299516649772	18.728609660511736	13.555296756738713	23.843098416251834
2	16.044654116893064	20.448583710286666	39.634462864802636	23.872299308017638
3	18.96122115379341	25.517910657897385	29.9088833791362	25.611984809173006
4	13.272042182195666	16.741187463867593	37.19144110350584	32.7953292504309
5	13.812166060712027	37.23026447734484	34.32349921234468	14.634070249598457
6	31.382936963908215	36.3099389455744	17.841109477086277	14.466014613431105
7	27.645492109808007	32.36904827340948	21.983096175995776	18.002363440786734
8	26.022923281810918	35.05981313153129	20.176284237906813	18.740979348750976
9	26.722874105553103	15.962988065432654	20.14942864074469	37.164709188269555
10-11	25.118654852281523	25.80268366290126	28.218176827469847	20.860484657347367
12-13	25.63849781165559	23.528032057892805	28.25124373006859	22.582226400383004
14-15	23.111446560604257	24.925531031589287	25.962510738120383	26.000511669686073
16-17	22.725010314870826	27.875257743209687	26.523928243353357	22.875803698566134
18-19	22.624120157455597	26.994619739521642	28.286692467401064	22.094567635621697
20-21	23.3733547249539	26.139268637641784	28.239647478221507	22.247729159182807
22-23	23.271833499854353	25.840523563571928	28.140947749349277	22.746695187224443
24-25	22.962130482341365	26.049009385729104	28.145651129528364	22.84320900240117
26-27	22.857282609186324	26.14123930887684	28.23793627830888	22.763541803627955
28-29	22.780216984651165	26.36777106395895	28.222581608098274	22.629430343291606
30-31	23.234084863584545	26.09657797066832	28.092206504627182	22.577130661119952
32-33	23.01702734515781	26.217965263710735	28.041728164190832	22.723279226940623
34-35	23.203775089740034	26.27669809241608	28.0036448559094	22.51588196193449
36-37	23.21075651125918	26.169670204691	27.976141363114937	22.64343192093488
38-39	23.013494384168087	26.37594824267196	27.912661051691845	22.697896321468107
40-41	23.092341527717544	26.24904505511396	27.96071769572156	22.697895721446933
42-43	22.945253245003098	26.37874813020958	27.72235171424191	22.95364691054541
44-45	22.856649488671415	26.400870960243612	27.78916526919748	22.953314281887497
46-47	22.954103519702812	26.32222161645484	27.591155704734348	23.132519159108003
48-49	22.98669716410721	26.455125723934653	27.483991443968353	23.074185667989784
50-51	22.888901918012753	26.66775928442223	27.43207740579629	23.011261391768727
52-53	22.980930299666717	26.710603119093847	27.227503567801076	23.08096301343836
54-55	22.798147576765288	26.533100209126125	27.41570388654622	23.253048327562368
56-57	22.892051740819593	26.64572442741344	27.212607077945094	23.24961675382188
58-59	22.89591786487155	26.52948718512785	27.366679749645606	23.207915200354996
60-61	22.988560259582748	26.566896800064498	27.453131416594324	22.99141152375843
62-63	22.805907534637942	26.676488940085846	27.3786015002537	23.139002025022513
64-65	22.700960896847725	26.479808915428666	27.39133127805349	23.42789890967012
66-67	22.69809690884679	26.47480679221073	27.26589965228483	23.561196646657645
68-69	22.526468652808944	26.548302762851705	27.35827062401816	23.56695796032119
70-71	22.665586684774038	26.4053401508714	27.285200469353306	23.64387269500126
72-73	22.617028926130793	26.59986025714605	27.050144691222922	23.732966125500237
74-75	22.562200501579945	26.761271134707908	26.96987326103705	23.706655102675093
76-77	22.51930509817788	26.531223891711452	27.038068609632372	23.911402400478295
78-79	22.478491571877743	26.485019496807404	27.129943784329118	23.90654514698573
80-81	22.54634953239789	26.688200028283248	26.84015250099634	23.92529793832252
82-83	22.577215566263675	26.57801870380252	26.948498462491006	23.8962672674428
84-85	22.57092209928134	26.631092864502243	26.81748878493309	23.980496251283324
86-87	22.38246782442935	26.68327257723797	26.956736430041968	23.97752316829072
88-89	22.284372092736234	26.502503625134004	26.98718995519335	24.225934326936404
90-91	22.558538213246678	26.421885158990715	26.88604720379722	24.13352942396539
92-93	22.486873022068384	26.422712315872772	26.880128866278323	24.21028579578052
94-95	22.386642931483657	26.245615719159186	26.922251276965987	24.445490072391166
96-97	22.25608794487949	26.209046677177216	27.099491324681402	24.435374053261892
98-99	22.239142478781844	26.206699164530033	26.92493615239499	24.629222204293136
100	22.220281985919218	26.16316848918091	26.97168324159241	24.644866283307465
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2248.0
1	1646.5
2	1994.5
3	2740.0
4	2011.0
5	1702.5
6	2420.5
7	4387.0
8	7145.5
9	8729.5
10	7869.0
11	6541.0
12	6663.5
13	7163.0
14	7780.0
15	8413.0
16	8961.0
17	9715.0
18	10521.0
19	11364.0
20	12381.0
21	14010.0
22	16303.0
23	19547.0
24	24040.5
25	29648.5
26	37263.0
27	46515.0
28	56589.0
29	68265.0
30	81376.5
31	94059.0
32	108340.5
33	124223.5
34	139746.0
35	154756.5
36	175402.0
37	196984.5
38	213065.0
39	228408.5
40	240430.5
41	251644.5
42	264581.5
43	278940.5
44	293724.0
45	309420.5
46	324785.5
47	340140.0
48	359544.0
49	368593.5
50	362942.0
51	356036.5
52	347699.0
53	338019.5
54	327743.0
55	309670.5
56	281129.5
57	251359.5
58	224566.5
59	195924.0
60	166137.0
61	138071.0
62	113080.0
63	88372.0
64	65896.5
65	49524.5
66	36647.5
67	27504.5
68	20690.0
69	14993.5
70	11395.5
71	8823.0
72	6651.5
73	5036.0
74	3814.0
75	2661.5
76	1779.0
77	1087.0
78	680.0
79	443.5
80	271.0
81	165.0
82	112.5
83	78.5
84	56.0
85	36.0
86	21.0
87	20.5
88	16.5
89	10.0
90	6.5
91	4.5
92	3.5
93	1.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030785749694713694
2	0.07762717062961771
3	0.002948301195707548
4	1.142752401437034E-5
5	0.00167984603011244
6	9.71339541221479E-4
7	1.142752401437034E-5
8	5.485211526897764E-4
9	3.428257204311102E-5
10-11	1.142752401437034E-5
12-13	9.713395412214789E-5
14-15	0.011616078160607451
16-17	9.884808272430344E-4
18-19	1.9998167025148095E-4
20-21	2.68546814337703E-4
22-23	0.0017712662222274026
24-25	7.999266810059238E-4
26-27	0.006976503410773093
28-29	0.007884991569915534
30-31	0.01002193856060279
32-33	0.009010602685331012
34-35	0.0044281655555685065
36-37	0.003931068260943397
38-39	0.003119714055923103
40-41	0.0033939746322679907
42-43	0.0017769799842345879
44-45	0.005256661046610356
46-47	0.017929785178547063
48-49	0.01888398343374699
50-51	0.013404485668856407
52-53	0.007593589707549092
54-55	0.0011656074494657746
56-57	0.0025769066652405115
58-59	0.0028168846695422888
60-61	0.0033996883942751764
62-63	0.003222561772052436
64-65	0.003782510448756583
66-67	0.0028454534795782148
68-69	0.0073136153691970174
70-71	4.3424591254607293E-4
72-73	0.005073820662380431
74-75	0.007445031895362277
76-77	0.006713670358442575
78-79	0.0045138719856762845
80-81	4.513871985676284E-4
82-83	0.0013884441677459963
84-85	8.456367770634052E-4
86-87	0.00117132121147296
88-89	0.001039904685307701
90-91	9.884808272430344E-4
92-93	2.9140186236644366E-4
94-95	9.084881591424421E-4
96-97	0.002628330523305178
98-99	0.0031768516759949545
100	0.004982400470265469
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	8750802.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.80441372352266
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.81317953431467	37.92052842931562
2	15.462116669284178	16.32936011292516
3	5.542345683838682	8.779809434645937
4	2.41708084391425	5.1053014754099735
5	1.3495062163782836	3.562994228605229
6	0.7955066790288684	2.520375827955955
7	0.5458639123192882	2.017681670399387
8	0.36304800618829614	1.5336429696213443
9	0.2607672245176116	1.2392694368066415
>10	1.3358211160914273	12.419223646342262
>50	0.07467823696766543	2.6821245377599925
>100	0.037605193770153936	3.5227355718722273
>500	0.001685603024208306	0.5861026433878322
>1k	6.360642900200182E-4	0.6042637514325326
>5k	6.360642895259125E-5	0.17503866039603808
>10k+	9.540964342888688E-5	1.0015476031237949
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25450	0.29083048616572515	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21676	0.24770301053549149	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12513	0.14299260799181607	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.142752401437034E-5
2	0.0	0.0	0.0	0.0	1.142752401437034E-5
3	0.0	0.0	0.0	0.0	1.142752401437034E-5
4	0.0	0.0	0.0	0.0	1.142752401437034E-5
5	0.0	0.0	0.0	0.0	1.142752401437034E-5
6	1.142752401437034E-5	0.0	0.0	0.0	1.142752401437034E-5
7	1.142752401437034E-5	0.0	0.0	0.0	1.142752401437034E-5
8	1.142752401437034E-5	0.0	0.0	0.0	1.142752401437034E-5
9	1.142752401437034E-5	0.0	0.0	0.0	2.285504802874068E-5
10-11	1.142752401437034E-5	0.0	0.0	0.0	2.285504802874068E-5
12-13	2.285504802874068E-5	0.0	0.0	1.142752401437034E-5	2.856881003592585E-5
14-15	2.285504802874068E-5	1.142752401437034E-5	0.0	2.285504802874068E-5	8.570643010777756E-5
16-17	2.285504802874068E-5	1.142752401437034E-5	0.0	2.285504802874068E-5	1.4284405017962927E-4
18-19	2.285504802874068E-5	1.142752401437034E-5	0.0	3.428257204311102E-5	1.4855781218681442E-4
20-21	2.285504802874068E-5	1.142752401437034E-5	0.0	8.570643010777754E-5	1.4855781218681442E-4
22-23	2.285504802874068E-5	1.142752401437034E-5	0.0	2.4569176630896233E-4	2.1140919426585128E-4
24-25	2.856881003592585E-5	1.714128602155551E-5	0.0	6.05658772761628E-4	2.68546814337703E-4
26-27	3.428257204311102E-5	2.285504802874068E-5	0.0	0.0013713028817244409	2.7997433835207336E-4
28-29	3.428257204311102E-5	2.285504802874068E-5	0.0	0.004685284845891839	2.856881003592585E-4
30-31	5.71376200718517E-5	2.285504802874068E-5	0.0	0.015855689569938845	2.856881003592585E-4
32-33	9.142019211496272E-5	2.856881003592585E-5	0.0	0.044435927129879066	3.085431483879992E-4
34-35	1.142752401437034E-4	4.571009605748136E-5	0.0	0.08823762667696058	3.199706724023695E-4
36-37	1.371302881724441E-4	4.571009605748136E-5	0.0	0.15592856517608328	3.256844344095547E-4
38-39	1.371302881724441E-4	5.142385806466653E-5	0.0	0.2486172124566411	3.428257204311102E-4
40-41	1.8284038422992544E-4	6.285138207903686E-5	0.0	0.3525562571293466	3.656807684598509E-4
42-43	1.8284038422992544E-4	6.856514408622204E-5	0.0	0.45624389627373585	3.771082924742212E-4
44-45	1.885541462371106E-4	6.856514408622204E-5	0.0	0.5926828192433105	3.942495784957767E-4
46-47	1.9426790824429577E-4	6.856514408622204E-5	0.0	0.7507026213140235	4.2281838853170257E-4
48-49	1.9426790824429577E-4	6.856514408622204E-5	0.0	0.9158874809417468	4.3424591254607293E-4
50-51	1.9426790824429577E-4	6.856514408622204E-5	0.0	1.088043130218236	4.3424591254607293E-4
52-53	2.2283671828022162E-4	6.856514408622204E-5	0.0	1.2647812166244876	4.6852848458918395E-4
54-55	2.285504802874068E-4	7.999266810059238E-5	0.0	1.463717268428654	5.085248186394801E-4
56-57	2.285504802874068E-4	7.999266810059238E-5	0.0	1.6955417343461776	5.256661046610356E-4
58-59	2.285504802874068E-4	7.999266810059238E-5	0.0	1.9511354502135918	5.428073906825912E-4
60-61	2.285504802874068E-4	9.142019211496272E-5	0.0	2.2160425981527183	5.485211526897764E-4
62-63	2.285504802874068E-4	9.142019211496272E-5	0.0	2.488937585377889	5.770899627257021E-4
64-65	2.285504802874068E-4	9.142019211496272E-5	0.0	2.765837919770097	5.828037247328873E-4
66-67	2.285504802874068E-4	9.142019211496272E-5	0.0	3.0824260450642127	5.885174867400725E-4
68-69	2.285504802874068E-4	9.142019211496272E-5	0.0	3.4365707280315565	5.942312487472577E-4
70-71	2.285504802874068E-4	9.142019211496272E-5	0.0	3.8123877102921537	5.942312487472577E-4
72-73	2.285504802874068E-4	9.142019211496272E-5	0.0	4.188421815509024	7.08506488890961E-4
74-75	2.285504802874068E-4	9.142019211496272E-5	0.0	4.572340912295811	7.08506488890961E-4
76-77	2.285504802874068E-4	9.142019211496272E-5	0.0	4.984228874107767	7.08506488890961E-4
78-79	2.285504802874068E-4	9.142019211496272E-5	0.0	5.438621511491176	7.08506488890961E-4
80-81	2.285504802874068E-4	9.142019211496272E-5	0.0	5.9155492262309215	7.37075298926887E-4
82-83	2.285504802874068E-4	9.142019211496272E-5	0.0	6.400041961868181	7.884991569915535E-4
84-85	2.285504802874068E-4	9.142019211496272E-5	0.0	6.887551563845234	7.884991569915535E-4
86-87	2.285504802874068E-4	9.142019211496272E-5	0.0	7.387722862430209	7.942129189987386E-4
88	2.285504802874068E-4	9.142019211496272E-5	0.0	7.781492484917383	8.113542050202942E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	18310	0.0	38.92343	1
GTATCAA	29210	0.0	34.27101	1
TCAACGC	34795	0.0	28.389374	4
ATCAACG	35415	0.0	27.920185	3
CAACGCA	35465	0.0	27.9193	5
TACCTGG	7815	0.0	27.502192	2
TATCAAC	36470	0.0	27.196991	2
AACGCAG	36780	0.0	26.984674	6
GTACATG	30305	0.0	26.606232	1
TACATGG	31710	0.0	24.946508	2
ACATGGG	32495	0.0	23.704031	3
ACGCAGA	41900	0.0	23.653212	7
CGCAGAG	43660	0.0	22.705164	8
GTACCTG	9845	0.0	22.314545	1
CATGGGG	24055	0.0	22.075628	4
ACCTGGG	9465	0.0	21.79735	3
GCAGAGT	47730	0.0	20.591805	9
GAGTACT	24940	0.0	20.47741	12-13
AGAGTAC	38625	0.0	20.085726	10-11
ATGGGGG	13455	0.0	19.558893	5
>>END_MODULE