FastQCFastQC Report
Mon 6 Jun 2016
ERR522982_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522982_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7446836
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201300.2703161450044019No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167990.2255857386949303No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88380.1186812761822605No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG93550.041.525622
ACCTGGG107450.035.8033683
GGTATCA181200.032.5993081
GTATCAA262550.030.5056041
GTACCTG134600.029.4200921
TCAACGC306150.025.82274
CAACGCA309950.025.5354275
ATCAACG311300.025.4111213
GTACATG254400.025.2158851
TATCAAC315250.025.2122442
AACGCAG320450.024.7751166
CCTGGGG108650.024.3695134
TACATGG260100.023.867862
TATAACG6600.023.5152682
ATAACGC7350.023.035213
ACATGGG259750.022.8497123
CATGGGG171750.022.2345054
ACGCAGA359450.021.9412737
CGCAGAG369250.021.3583728
CCGTATC36150.020.92767194
Produced by FastQC (version 0.11.5)