FastQCFastQC Report
Mon 6 Jun 2016
ERR522982_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522982_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7446836
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194840.26164131988404205No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168210.22588116617580944No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT156400.21002208186134352No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89310.11993012871506772No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT75010.1007273424579244RNA PCR Primer, Index 31 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG95400.041.6853142
ACCTGGG110300.035.200443
GGTATCA184500.034.3411871
GTACCTG135750.029.6951161
GTATCAA268900.029.4574641
CCTGGGG101250.026.415444
TATAACG6850.025.3905562
TAACGCA7300.025.1120624
TCAACGC308800.025.0244874
CAACGCA312050.024.839675
ATCAACG314350.024.5977863
ATAACGC6900.024.5243193
TATCAAC319850.024.2198752
AACGCAG322850.024.1512496
GTACATG248900.023.6604771
TACATGG250800.023.0723362
ACATGGG256350.021.5084083
ACGCAGA362800.021.4791497
CGCAGAG376850.020.6647648
CATGGGG164900.020.637574
Produced by FastQC (version 0.11.5)