FastQCFastQC Report
Mon 6 Jun 2016
ERR522981_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522981_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8794838
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281980.32061989089509096No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227660.2588563882586581No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124840.14194690112541017No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA269350.027.8302461
GTATCAA381950.027.554931
TACCTGG89750.025.5184972
GTACATG318400.024.0008181
TCAACGC434800.023.9360454
CAACGCA440150.023.643495
ATCAACG443250.023.4692633
TATCAAC448300.023.341132
TACATGG323000.023.0919822
AACGCAG451950.023.047956
GTACCTG103700.022.9012051
ACATGGG332400.021.859013
CATGGGG208150.021.0797654
ATAACGC9750.020.7408643
ACGCAGA506250.020.5280177
GAGTACT255650.020.47089612-13
CGCAGAG519950.019.9681438
ACCTGGG109350.019.9124743
GTACTTT278650.018.5799214-15
AGTACTT269500.018.34633812-13
Produced by FastQC (version 0.11.5)