FastQCFastQC Report
Mon 6 Jun 2016
ERR522981_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522981_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8794838
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269390.30630467553808266No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237810.2703972489317029No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124830.14193553081932833No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA270200.029.8699781
GTATCAA378650.027.5751571
TACCTGG86150.026.3527282
TCAACGC439300.023.4746574
CAACGCA445050.023.1506395
GTACCTG101500.023.122641
ATCAACG447850.023.0160033
TATCAAC452500.022.8527162
AACGCAG458200.022.5763766
GTACATG318400.021.3450781
TACATGG319500.020.699362
ACCTGGG105900.020.549863
GAGTACT268850.020.44382512-13
ACGCAGA515450.020.0322767
ACATGGG322950.019.7646123
CGCAGAG529550.019.4805858
GTACTTT283050.019.0943214-15
CATGGGG204950.018.920384
AGAGTAC438650.017.95160310-11
GCAGAGT571400.017.897539
Produced by FastQC (version 0.11.5)