FastQCFastQC Report
Mon 6 Jun 2016
ERR522980_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522980_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9414173
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249480.2650046902685982No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195140.20728321011309223No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127040.13494546998445855No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG140400.051.6863672
ACCTGGG152000.046.9985473
GTACCTG198750.037.1503371
CCTGGGG148150.033.9981884
GGTATCA209300.030.9185731
GTACATG324850.029.8232251
GTATCAA306150.029.755391
TACATGG329700.028.81442
CATGGGG216300.028.0696954
ATGGGGG146100.027.7475725
ACATGGG327900.027.738653
TGGGGGG165450.026.5084426
TATAACG8150.025.3905582
CTGGGGG100600.025.3378185
TCAACGC355950.025.3280094
CAACGCA358050.025.2188635
ATCAACG361850.024.8761733
AACGCAG370250.024.6052656
TATCAAC368200.024.4347762
ATAACGC9500.023.7622663
Produced by FastQC (version 0.11.5)