FastQCFastQC Report
Mon 6 Jun 2016
ERR522980_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522980_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9414173
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241650.25668744349609895No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210750.22386459224830474No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131300.1394705620982321No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT106080.11268116700213604No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG139500.054.5187952
ACCTGGG157050.047.2576683
GTACCTG207350.037.3338781
CCTGGGG148950.035.0274284
GGTATCA221350.031.4030761
TATAACG8600.028.9680562
GTATCAA317900.028.5524981
TGGGGGG150550.027.4408136
CTGGGGG93650.027.25385
GTACATG328550.026.4245451
ATAACGC10650.026.0393713
TAACGCA9800.025.8996244
TACATGG334850.025.5343232
CATGGGG215400.025.356324
ATGGGGG144000.025.0035275
ACATGGG331700.024.6423533
TCAACGC370850.023.9926434
ATCAACG377150.023.6543053
CAACGCA376750.023.6423685
TATCAAC387550.023.1779442
Produced by FastQC (version 0.11.5)