FastQCFastQC Report
Mon 6 Jun 2016
ERR522979_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522979_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4445402
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136510.30708133932544235No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114960.25860428370707533No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61190.1376478437720593No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG48800.034.406352
GGTATCA129450.029.5008411
ACCTGGG55900.029.1946323
GTATCAA184650.028.322781
TATAACG4950.027.5538962
GTACCTG65750.026.3228241
GTACATG153100.025.9574451
TAACGCA5000.025.3965174
TACATGG158450.024.6956982
TCAACGC210100.024.6233464
CAACGCA211400.024.3823835
ATCAACG213650.024.170453
AACGCAG218550.023.7665926
TATCAAC219150.023.6499582
ATAACGC5800.023.5155623
ACATGGG159850.023.3905143
CATGGGG100150.022.7287124
ACGCAGA245050.021.0406177
GAGTACT127400.020.62100412-13
CGCAGAG251150.020.5103998
Produced by FastQC (version 0.11.5)