FastQCFastQC Report
Mon 6 Jun 2016
ERR522979_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522979_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4445402
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122880.2764204452150784No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110760.24915631927101303No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58310.1311692395873309No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT51510.11587253526227773No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT48910.11002379537328683No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG48400.036.223632
ACCTGGG56200.029.94063
TATAACG3150.029.8433722
GGTATCA130150.028.2940621
GTACCTG66150.027.3010351
ATAACGC4600.025.5444163
GTACATG147500.025.5398431
GTATCAA177300.025.438271
TAACGCA3900.025.3089074
TACATGG149700.024.6790942
ACATGGG149450.023.2099533
CATGGGG95000.021.7694664
CGAGACT15250.021.72622512-13
TCAACGC204400.021.5695294
ATCAACG206450.021.3095743
CAACGCA207450.021.2526445
ATGGGGG57750.021.1614285
TATCAAC213450.020.9197222
CCTGGGG56450.020.8158784
AACGCAG213200.020.7219226
Produced by FastQC (version 0.11.5)