FastQCFastQC Report
Mon 6 Jun 2016
ERR522978_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522978_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8747725
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209230.23918218736871588No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170540.19495354506457394No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101600.1161444832799385No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115250.051.274481
GTATCAA211050.042.601791
TCAACGC258050.033.9456524
CAACGCA262850.033.271555
ATCAACG265150.033.110043
AACGCAG273350.032.193176
TATCAAC274200.032.141642
GTACATG285400.028.4042871
ACGCAGA312600.028.0608237
TACATGG292850.027.2537732
CGCAGAG327500.026.6399388
ACATGGG300300.025.6506333
CATGGGG217950.023.7395534
GCAGAGT364200.023.3866569
GAGTACT209900.020.53672812-13
GTGGTAT62350.020.2232461
CAGAGTA355400.019.93352910-11
AGAGTAC330800.019.9247310-11
ATGGGGG126700.018.7675915
TGGTATC64850.018.5551382
Produced by FastQC (version 0.11.5)