FastQCFastQC Report
Mon 6 Jun 2016
ERR522978_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522978_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8747725
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195790.22381819273010983No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173640.19849732358984767No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99430.1136638383122469No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117450.054.0429421
GTATCAA210050.041.1272851
TCAACGC260600.032.531434
ATCAACG265000.031.9393623
CAACGCA266000.031.906355
TATCAAC274100.031.1285722
AACGCAG278100.030.6358816
ACGCAGA320400.026.5909547
GTACATG286950.026.5309221
TACATGG294450.025.4788652
CGCAGAG336800.025.2458178
ACATGGG300050.024.2927443
CATGGGG220650.022.4267044
GCAGAGT382300.022.1672539
GTGGTAT62650.021.7799571
GAGTACT214250.019.97495512-13
AGAGTAC341800.019.61666310-11
CAGAGTA369900.019.49880610-11
ATGGGGG127750.019.3861145
GTACTTT232000.018.21178214-15
Produced by FastQC (version 0.11.5)