FastQCFastQC Report
Mon 6 Jun 2016
ERR522977_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522977_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6983973
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204900.29338601394936664No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164630.2357254244825975No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93070.13326225631170108No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100350.053.683911
GTATCAA186000.042.3193551
TCAACGC224550.033.9657064
CAACGCA228600.033.3852275
ATCAACG229750.033.2207453
TATCAAC239450.032.213742
AACGCAG240150.031.9290146
ACGCAGA274800.027.834647
GTACATG240150.026.6839771
CGCAGAG287000.026.601178
TACATGG249800.025.27152
ACATGGG254200.024.0721193
GCAGAGT316000.023.5187599
CATGGGG174250.022.4920224
GAGTACT184650.020.53378712-13
CAGAGTA309400.020.40587610-11
AGAGTAC290050.019.96946710-11
TACCTGG52750.019.6932432
GTGGTAT54300.019.5823441
ATGGGGG101550.019.2512075
Produced by FastQC (version 0.11.5)