FastQCFastQC Report
Mon 6 Jun 2016
ERR522977_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522977_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6983973
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186120.26649587562838517No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158630.22713432597749159No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90140.12906693654170773No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98650.053.6516951
GTATCAA178450.042.1296841
TCAACGC221800.033.3706634
CAACGCA227250.032.570355
ATCAACG229300.032.1368523
AACGCAG237400.031.3157066
TATCAAC238200.031.116892
ACGCAGA274550.026.9921447
GTACATG234700.026.0991231
CGCAGAG284750.025.949258
TACATGG242350.024.5875532
ACATGGG244100.023.6231653
GCAGAGT320000.022.914519
CATGGGG172500.021.8489744
GTGGTAT54250.021.246841
TACCTGG54900.020.9882132
GAGTACT187550.020.38823312-13
CAGAGTA308850.020.08925610-11
AGAGTAC293150.020.08285110-11
TGGTATC52350.019.9442652
Produced by FastQC (version 0.11.5)