FastQCFastQC Report
Mon 6 Jun 2016
ERR522976_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522976_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9359265
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232860.2488015885862832No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185330.1980176862178814No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101240.10817088735066269No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130900.049.600091
GTATCAA229300.041.9802061
TCAACGC280650.033.9069444
CAACGCA285550.033.308475
ATCAACG287350.033.11993
TATCAAC294200.032.401192
AACGCAG299200.031.8604166
GTACATG316950.028.441191
ACGCAGA342700.027.761737
TACATGG326350.026.9911672
CGCAGAG360300.026.4301438
ACATGGG339450.025.3644163
TACCTGG77800.024.6499612
GCAGAGT401400.023.2072059
CATGGGG245750.023.0229844
GAGTACT219800.020.6589712-13
GTGGTAT73450.020.561571
CAGAGTA387050.019.9349810-11
AGAGTAC362400.019.56691710-11
ATGGGGG151400.019.5232055
Produced by FastQC (version 0.11.5)