FastQCFastQC Report
Mon 6 Jun 2016
ERR522976_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522976_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9359265
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201980.2158075447163853No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174870.18684159493293542No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA122400.050.237981
GTATCAA221700.041.9120981
TCAACGC273150.033.445394
ATCAACG278550.032.8321573
CAACGCA279050.032.755095
TATCAAC287700.031.9137672
AACGCAG292450.031.3977036
ACGCAGA340600.026.9313287
GTACATG316750.026.5130461
TACATGG322850.025.5107672
CGCAGAG360050.025.430678
TACCTGG82350.024.78932
ACATGGG337400.023.6372263
GCAGAGT409400.022.2500329
CATGGGG246250.021.7746224
GTGGTAT71650.020.3555831
GTACCTG105950.019.9824891
GAGTACT219150.019.81810612-13
ATGGGGG143250.019.6176855
AGAGTAC364900.019.40554210-11
Produced by FastQC (version 0.11.5)