FastQCFastQC Report
Mon 6 Jun 2016
ERR522975_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522975_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5781620
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145270.2512617570853844No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121190.20961253074397831No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73490.12710970281685757No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79350.050.2735751
GTATCAA138900.039.9641531
TACCTGG61550.038.258472
TCAACGC170800.031.8882474
ATCAACG175200.031.1169193
ACCTGGG73750.031.0341343
CAACGCA175800.030.9273015
GTACCTG79050.030.6475811
TATCAAC179600.030.4886022
AACGCAG183000.030.1156066
GTACATG191000.029.3092331
TACATGG195450.027.871842
ACATGGG201100.026.5250663
ACGCAGA210500.026.1143047
CATGGGG146350.025.1743564
CGCAGAG220200.025.0044548
ATGGGGG93650.023.4334725
GCAGAGT244600.021.9512469
TATAACG4500.020.889852
GAGTACT142850.019.99602112-13
Produced by FastQC (version 0.11.5)