FastQCFastQC Report
Mon 6 Jun 2016
ERR522975_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522975_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5781620
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134740.2330488686561898No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117040.20243461175241542No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72420.1252590104503582No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77350.051.9993821
GTATCAA138300.040.375781
TACCTGG62350.039.4474142
TCAACGC171400.031.9964264
GTACCTG78450.031.72161
CAACGCA175050.031.356115
ATCAACG175700.031.240923
ACCTGGG79650.030.3270423
TATCAAC182300.030.2854982
AACGCAG183800.029.9395526
GTACATG196600.028.8094751
TACATGG203000.027.4289342
ACATGGG204800.026.228233
ACGCAGA215400.025.4166267
CATGGGG148250.024.4716934
CGCAGAG225650.024.264828
ATGGGGG92600.023.34475
ATAACGC5650.022.4578423
TGGGGGG84050.022.3643446
GCAGAGT250750.021.704539
Produced by FastQC (version 0.11.5)